Dysfunctional mitochondria regulate the size of root apical meristem and leaf development in Arabidopsis

Hsieh, Wei‐Yu; Liao, Jo-Chien; Hsieh, Ming‐Hsiun

doi:10.1080/15592324.2015.1071002

Cited by 17 publications

(15 citation statements)

References 39 publications

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“…Thus, the direct outcome of AOX activity might be the oxidation of the ubiquinol pool (Vanlerberghe et al 2016). In agreement with this hypothesis, rcd1 phenotypically resembles mitochondrial mutants with oxidized ubiquinol pools that are often characterized by curly leaves (Hsieh et al, 2015)(Fig. 4C).…”

Section: Discussionsupporting

confidence: 79%

RCD1 Coordinates Chloroplastic and Mitochondrial Electron Transfer through Interaction with ANAC Transcription Factors in Arabidopsis

Shapiguzov

Vainonen

Hunter

et al. 2018

Preprint

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43Signaling from chloroplasts and mitochondria, both dependent on reactive oxygen 44 species (ROS), merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 45 (RCD1). ROS produced in the chloroplasts affect the abundance, thiol redox state and 46 oligomerization of RCD1. RCD1 directly interacts in vivo with ANAC013 and ANAC017 47 transcription factors, which are the mediators of the ROS-related mitochondrial complex 48 III retrograde signa and suppresses activity of ANAC013 and ANAC017. Inactivation of 49 RCD1 leads to increased expression of ANAC013 and ANAC017-regulated genes 50 belonging to the mitochondrial dysfunction stimulon (MDS), including genes for 51 mitochondrial alternative oxidases (AOXs). Accumulating AOXs and other MDS gene 52 products alter electron transfer pathways in the chloroplasts, leading to diminished 53 production of chloroplastic ROS and increased protection of photosynthetic apparatus 54 from ROS damage. RCD1-dependent regulation affects chloroplastic and mitochondrial 55 retrograde signaling including chloroplast signaling by 3'-phosphoadenosine 5'-56 phosphate (PAP). Sensitivity of RCD1 to organellar ROS provides feedback control of 57 nuclear gene expression. 58 157 Nishiyama et al., 2011).To reveal the significance of RCD1 in responses to PSI-produced 158 chloroplastic ROS, rosettes of Arabidopsis were pre-treated overnight in darkness with 159 MV and exposed to light. After the dark period, the plants displayed unchanged PSII 160 photochemical yield (Fv/Fm). Subsequent exposure to three hours of light resulted in 161 decrease of Fv/Fm in wild type (Col-0) (Fig. 1A), but not in the rcd1 mutant. Moreover, 162 tolerance of rcd1 was evident under various concentrations of MV (Fig. 1B). The 163 superoxide anion is an unstable compound that is enzymatically reduced to the more 164 long-lived H2O2. Chloroplastic production of H2O2 in the presence of MV in the light was 165 assessed by staining plants with 3,3′-diaminobenzidine (DAB). After pre-treatment with 166 MV, higher H2O2 accumulation was evident in both Col-0 and rcd1 (Fig. S1A). Subsequent 167 incubation of these plants under light led to a time-dependent increase in the H2O2 168accumulation in Col-0, but not in rcd1. 169 In several MV-tolerant mutants the resistance is based on restricted access of MV to 170 chloroplasts (Hawkes 2014). However, in rcd1 pretreatment with MV led to initial increase 171 in H2O2 production similar to that in the wild type ( Fig. S1A), suggesting that resistance 172 of rcd1 was not due to restricted delivery of MV to PSI. In order to test this directly, 173 oxidation of PSI was assessed by in vivo spectroscopy using DUAL-PAM. Leaves were 174 adapted to far-red light, which is more efficiently used by PSI than PSII. Under these 175 conditions PSI is producing electrons at a faster rate than it is supplied by electrons 176 coming from PSII, and hence the PSI reaction center P700 becomes oxidized. Then a 177 flash of orange light was provided that is efficiently absorbed by PSII. Electrons generated...

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Section: Discussionsupporting

confidence: 79%

RCD1 Coordinates Chloroplastic and Mitochondrial Electron Transfer through Interaction with ANAC Transcription Factors in Arabidopsis

Shapiguzov

Vainonen

Hunter

et al. 2018

Preprint

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“…Mitochondria play an important role in energy-, redox-, and metabolic homeostasis, and have been shown to be integrated with plant growth regulation. Most mitochondrial mutants revealed reduced growth rates and have a short root phenotype [51]. Even though it is not clear how the TOM machinery might control the root development, elevated TOM40 levels appeared to be associated with increased root growth.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Profiling of the Microsomal Root Fraction: Discrimination of Pisum sativum L. Cultivars and Identification of Putative Root Growth Markers

2017

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Legumes are a large and economically important family, containing a variety of crop plants. Alongside different cereals, some fruits, and tropical roots, a number of leguminosae evolved for millennia as crops with human society. One of these legumes is Pisum sativum L., the common garden pea. In the past, breeding has been largely selective on improved above-ground organs. However, parameters, such as root-growth, which determines acquisition of nutrients and water, have largely been underestimated. Although the genome of P. sativum is still not fully sequenced, multiple proteomic studies have been published on a variety of physiological aspects in the last years. The presented work focused on the connection between root length and the influence of the microsomal root proteome of four different pea cultivars after five days of germination (cultivar Vroege, Girl from the Rhineland, Kelvedon Wonder, and Blauwschokker). In total, 60 proteins were identified to have significantly differential abundances in the four cultivars. Root growth of five-days old seedlings and their microsomal proteome revealed a similar separation pattern, suggesting that cultivar-specific root growth performance is explained by differential membrane and ribosomal protein levels. Hence, we reveal and discuss several putative root growth protein markers possibly playing a key role for improved primary root growth breeding strategies.

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“…3B). Slower root growth has been observed in several other Arabidopsis mutants with altered mitochondrial biogenesis (Hsieh et al, 2015;Radin et al, 2015).…”

Section: Oxa2bδ235 Complementation Plants Have a Severe Growth Retardmentioning

confidence: 99%

OXA2b is Crucial for Proper Membrane Insertion of COX2 during Biogenesis of Complex IV in Plant Mitochondria

2018

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The evolutionarily conserved YidC/Oxa1/Alb3 proteins are involved in the insertion of membrane proteins in all domains of life. In plant mitochondria, individual knockouts of OXA1a, OXA2a, and OXA2b are embryo-lethal. In contrast to other members of the protein family, OXA2a and OXA2b contain a tetratricopeptide repeat (TPR) domain at the C-terminus. Here, the role of Arabidopsis (Arabidopsis thaliana) OXA2b was determined by using viable mutant plants that were generated by complementing homozygous lethal OXA2b T-DNA insertional mutants with a C-terminally truncated OXA2b lacking the TPR domain. The truncated-OXA2b-complemented plants displayed severe growth retardation due to a strong reduction in the steady-state abundance and enzyme activity of the mitochondrial respiratory chain complex IV. The TPR domain of OXA2b directly interacts with cytochrome c oxidase subunit 2, aiding in efficient membrane insertion and translocation of its C-terminus. Thus, OXA2b is crucial for the biogenesis of complex IV in plant mitochondria.

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Dysfunctional mitochondria regulate the size of root apical meristem and leaf development in Arabidopsis

Cited by 17 publications

References 39 publications

RCD1 Coordinates Chloroplastic and Mitochondrial Electron Transfer through Interaction with ANAC Transcription Factors in Arabidopsis

RCD1 Coordinates Chloroplastic and Mitochondrial Electron Transfer through Interaction with ANAC Transcription Factors in Arabidopsis

Proteomic Profiling of the Microsomal Root Fraction: Discrimination of Pisum sativum L. Cultivars and Identification of Putative Root Growth Markers

OXA2b is Crucial for Proper Membrane Insertion of COX2 during Biogenesis of Complex IV in Plant Mitochondria

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