Dysbiosis signatures of the microbial profile in tissue from bladder cancer

Abstract

BackgroundTo examine the microbial profiles in parenchyma tissues in bladder cancer.MethodsTissue samples of cancerous bladder mucosa were collected from patients diagnosed with bladder cancer (22 carcinoma tissues and 12 adjacent normal tissues). The V3‐V4 region of the bacterial 16S rRNA gene was PCR amplified, followed by sequencing on an Illumina MiSeq platform. Bioinformatics analysis for microbial classification and functional assessment was performed to assess bladder microbiome diversity and variations.ResultsThe predominant phylum in both tissues was Proteobacteria. The cancerous tissues exhibited lower species richness and diversity. Beta diversity significantly differed between the cancerous and normal tissues. Lower relative abundances of the microbial genera Lactobacillus, Prevotella_9, as well as Ruminococcaceae were observed, whereas those of Cupriavidus spp., an unknown genus of family Brucellaceae, and Acinetobacter, Anoxybacillus, Escherichia‐Shigella, Geobacillus, Pelomonas, Ralstonia, and Sphingomonas were higher in the cancerous tissues. These findings indicate that these genera may be potentially utilized as biomarkers for bladder cancer. PICRUSt analysis revealed that several pathways involved in the metabolism of harmful chemical compounds were enriched in the cancer tissues, thereby providing evidence that environmental factors are strongly associated with bladder cancer etiology.ConclusionThis is the first study that has described and analyzed the dysbiotic motifs of urinary microbiota in the parenchymatous tissues of bladder cancer via 16S rRNA gene sequencing. Our results suggest that changes in the bladder microbiome may serve as biomarkers for bladder cancer, possibly assisting in disease screening and monitoring.

Alterations in Urobiome in Patients With Bladder Cancer and Implications for Clinical Outcome: A Single-Institution Study

“…Our previous study (Wu et al, 2018) has shown increased bacterial richness, enrichment of some bacterial genera (e.g., Acinetobacter, Anaerococcus, and Sphingobacterium) and decrease of some bacterial genera (e.g., Serratia, Proteus, and Roseomonas) in bladder cancer group when compared with non-cancer group. It is worthy of note that higher abundance of the Acinetobacter in bladder cancer samples was also presented in several other studies (Liu et al, 2019;Mai et al, 2019). Acinetobacter components could play a role in carcinogenesis, by the activation of NF-kB, in animal models of bladder cancer (Roperto et al, 2012).…”

“…It was reported that a higher abundance of Actinomyces was presented in bladder cancer patients, while Bifidobacterium, Streptococcus, Lactobacillus, and Veillonella were enriched in the control group (Bi et al, 2019). Liu et al revealed higher relative abundances of the several microbial genera, such as Acinetobacter and Anoxybacillus, in the cancerous compared to the normal tissues (Liu et al, 2019). Our previous study (Wu et al, 2018) has shown increased bacterial richness, enrichment of some bacterial genera (e.g., Acinetobacter, Anaerococcus, and Sphingobacterium) and decrease of some bacterial genera (e.g., Serratia, Proteus, and Roseomonas) in bladder cancer group when compared with non-cancer group.…”

Gastric Mucosa-Associated Microbial Signatures of Early Gastric Cancer

“…Novosphingobium, Ralstonia, Ochrobactrum, Anoxybacillus, and Pseudoxanthomonas were enriched in EC. Previous studies have found Novosphingobium, Anoxybacillus, and Ralstonia are associated with gastric cancer, extrahepatic cholangiocarcinoma and bladder cancer (Avilés-Jiménez et al, 2016;Tseng et al, 2016;Liu et al, 2019a). They play a role in the initiation of inflammation (Rutebemberwa et al, 2014;Tejera et al, 2016).…”

The effects of exopolysaccharides and exopolysaccharide-producing Lactobacillus on the intestinal microbiome of zebrafish (Danio rerio)

“…Vibrio and Cetobacterium are also known as core microbiota of adult zebrafish [ 29 ], which is consistent with our study. L. fermentum HNUB20 inhibited the growth of pathogenic bacteria ( Ochrobactrum ) [ 30 , 31 ], and bacteria associated with disease biomarkers ( Sediminibacterium , Sphingomonas , Sphingobium ) [ 32 – 34 ]. HNUB20 derived EPS can decrease the level of Pelomonas , which is a biomarker of bladder cancer [ 32 ].…”

“…L. fermentum HNUB20 inhibited the growth of pathogenic bacteria ( Ochrobactrum ) [ 30 , 31 ], and bacteria associated with disease biomarkers ( Sediminibacterium , Sphingomonas , Sphingobium ) [ 32 – 34 ]. HNUB20 derived EPS can decrease the level of Pelomonas , which is a biomarker of bladder cancer [ 32 ]. It also inhibited the growth of Brachybacterium , which is an end-stage renal disease biomarker ( Brachybacterium ) [ 35 ].…”