Dysbiosis of the rat vagina is efficiently rescued by vaginal microbiota transplantation or probiotic combination

Chen, Tingtao; Xia, Chaofei; Hu, Hong; Wang, Huan; Tan, Buzhen; Tian, Puyuan; Zhao, Xiaoxiao; Wang, Le; Han, Yiwen; Deng, Ke-Yu; Wei, Hong; Xin, Hong‐Bo

doi:10.1016/j.ijantimicag.2021.106277

Cited by 36 publications

(25 citation statements)

References 47 publications

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“…There has also been a growing interest in vaginal microbial transplantation (VMT) in recent years ( Korpela et al., 2020 ; Wang et al., 2021 ). One study ( Chen et al., 2021 ) investigated the effect of VMT on vaginal dysbiosis by establishing a vaginal dysbiosis model. The results showed that VMT significantly reduced bacterial-induced inflammation, as well as the enrichment of pro-inflammatory cytokines, and restored normal vaginal microbiota.…”

Section: Vaginal Microbial Transplantationmentioning

confidence: 99%

The role of probiotics in vaginal health

Mei¹,

2022

Front. Cell. Infect. Microbiol.

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Probiotics have been widely used in the treatment of intestinal diseases, but the effect of probiotics on female reproductive tract health is still controversial. Lactobacillus is the most abundant microorganism in the vagina, which is related to the vaginal mucosal barrier. Lactobacillus adheres to the vaginal epithelium and can competitively antagonize the colonization of pathogens. The factors produced by Lactobacillus, such as bacteriocin and hydrogen peroxide (H2O2), can inhibit the growth of pathogenic microorganisms and maintain the low pH environment of the vagina. Probiotics play an important role in maintaining the stability of vaginal microenvironment, improving immune defense and blocking the progression of cervical cancer. We review the research progress of probiotics represented by Lactobacillus in gynecological diseases such as human papilloma virus (HPV) infection, bacterial vaginosis (BV) and Genitourinary Syndrome of Menopause (GSM), so as to provide basis for further exerting the role of probiotics in women’s health.

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Section: Vaginal Microbial Transplantationmentioning

confidence: 99%

The role of probiotics in vaginal health

Mei¹,

2022

Front. Cell. Infect. Microbiol.

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“…The removal of vaginal microorganisms by antibiotics from mice with endometriosis could inhibit the progress of this disease, and thus, it is reasonable to hypothesis that the restoration of normal vaginal microecology has an advantageous impact on endometriotic lesions. VMT, a promising technique that grafting the whole vaginal microbiota from a healthy donor to the receptor with vaginal microbiota dysbiosis, has been studied in various gynecological diseases and has been applied to treat intractable BV of rats and humans ( 30 , 41 , 42 ). LA, one of the gonadotropin-releasing hormone analogs, is the first-line medicine for the clinical treatment of endometriosis.…”

Section: Discussionmentioning

confidence: 99%

“…The absorbent gelatin sponge was cut into small squares and then placed in PBS suspension containing normal vaginal microbiota to make it fully contact with the bacterial solution. The sponge was inserted into the uterine cavity through the vagina with tweezers and repeated every 3 days ( 18 , 30 ).…”

Section: Methodsmentioning

confidence: 99%

Antibiotic Therapy and Vaginal Microbiota Transplantation Reduce Endometriosis Disease Progression in Female Mice via NF-κB Signaling Pathway

Wei

Zhong

et al. 2022

Front. Med.

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Endometriosis (EMS) is a disease characterized by estrogen-dependent, chronic inflammatory, and annoying symptoms, which inflicts about 10% reproductive-age women. The diagnosis of endometriosis mainly depends on pathological examination after surgical resection while the pathogenesis of EMS is not clear enough. Surgical resection and drug therapy (including painkillers and hormone therapy, especially gonadotropin-releasing hormone analogs, GnRH-a) are widely used, but they are expensive and have many side effects. There are few studies on vaginal microorganisms in women with endometriosis. We collected vaginal secretions from women with EMS confirmed by pathology and demonstrated that they were different from that of healthy women by 16s rRNA high-throughput sequencing. Additionally, we established the EMS model in female mice by intraperitoneally injecting fragments from donor mice (3-week growth). Then, the mice were treated with mixed antibiotics (vagina) and NF-κB signaling pathway inhibitors (intraperitoneal injection), respectively. The result suggested that the ectopic lesions were inhibited. In addition, inflammatory cytokines IL-1β, IL-6, and TNF-α in peritoneal fluid, cell proliferation marker ki-67, and macrophage marker Iba-1 in ectopic lesions decreased significantly from that of mock mice. We also observed similar results as above by vaginal microbiota transplantation (VMT) and subcutaneous injection of leuprorelin acetate (LA, one of GnRH-a) for mice with EMS. These results showed that vaginal use of antibiotics or VMT is helpful to treat endometriosis in mice. However, due to the great difference between human and mouse vaginal microbiota, its mechanism and clinical transformation application still need to be further studied in the future.

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“…Besides, the authors also found that patients with long-term resolution of BV had a dramatic change in microbial composition in the first month after VMT, which was dominated by increase of Lactobacillus and decrease of Bifidobacterium (closely related to Gardnerella), accompanied with reduced Fannyhessea and Prevotella. In 2021, our group verified the feasibility of VMT in animal models and explored the specific mechanisms (Chen et al, 2021). The results showed that vaginal secretions from healthy rats can be used to treat vaginal microbiota imbalance and prevent the recurrence in rats, which specifically manifested as the decrease of inflammatory cells, pro-inflammatory cytokines, and apoptotic factors in the uterine wall and the restoration of the diversity of vaginal microbiota.…”

Section: Frontiers and Conclusion In Treating Vaginal Dysbacteriosismentioning

confidence: 95%

Role of Vaginal Microbiota Dysbiosis in Gynecological Diseases and the Potential Interventions

Han

Chen

2021

Front. Microbiol.

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Vaginal microbiota dysbiosis, characterized by the loss of Lactobacillus dominance and increase of microbial diversity, is closely related to gynecological diseases; thus, intervention on microbiota composition is significant and promising in the treatment of gynecological diseases. Currently, antibiotics and/or probiotics are the mainstay of treatment, which show favorable therapeutic effects but also bring problems such as drug resistance and high recurrence. In this review, we discuss the role of vaginal microbiota dysbiosis in various gynecological infectious and non-infectious diseases, as well as the current and potential interventions.

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Dysbiosis of the rat vagina is efficiently rescued by vaginal microbiota transplantation or probiotic combination

Cited by 36 publications

References 47 publications

The role of probiotics in vaginal health

The role of probiotics in vaginal health

Antibiotic Therapy and Vaginal Microbiota Transplantation Reduce Endometriosis Disease Progression in Female Mice via NF-κB Signaling Pathway

Role of Vaginal Microbiota Dysbiosis in Gynecological Diseases and the Potential Interventions

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