Dysbiosis of the fecal microbiota in feedlot cattle with hemorrhagic diarrhea

Zeineldin, Mohamed; Aldridge, Brian; Lowe, James

doi:10.1016/j.micpath.2017.12.059

Cited by 64 publications

(41 citation statements)

References 32 publications

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“…Those numerous microorganisms are of great importance for acting the role of gastrointestinal system protection, synthesizing and metabolizing parts of NHVs (nutrients, hormones, and vitamins), eliminating drugs and toxic metabolites, inducting and regulating host responses to various pathogens, protecting from pathogens, and helping with the development and maturation of immune cells [3–7]. The changes or disturbances of microbial flora were found to have relationships with various gastrointestinal diseases, which may cause impaired digestive, diminished weight gain, being even mortal to animals [5]. Infectious diseases may cause serious disaster for animal health and productivity in developing countries [8–10].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Intestinal Microbial Community in Ducks Reared Differently through High-Throughput Sequencing

Zhao

Luo

et al. 2019

BioMed Research International

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Birds are an important source of fecal contamination in environment. Many of diseases are spread through water contamination caused by poultry droppings. A study was conducted to compare the intestinal microbial structure of Shaoxing ducks with and without water. Thirty 1-day-old Shaoxing ducks (Qingke No. 3) were randomly divided into two groups; one group had free access to water (CC), while the other one was restricted from water (CT). After 8 months of breeding, caecal samples of 10 birds from each group were obtained on ice for high-throughput sequencing. A total of 1507978 valid sequences were examined and clustered into 1815 operational taxonomic units (OTUs). At phylum level, Firmicutes (41.37%), Bacteroidetes (33.26%), Proteobacteria (13.67%), and Actinobacteria (8.26%) were found to dominate the microbial community in CC birds, while Firmicutes (53.62%), Bacteroidetes (33.06%), and Actinobacteria (11.13%) were uncovered to be the prime phyla in CT ducks. At genus level, Bacteroides (25.02%), Escherichia-Shigella (11.02%), Peptococcus (7.73%) and Parabacteroides (5.86%) were revealed to be the mainly genera in the CC group ducks, while Bacteroides (18.11%), Erysipelatoclostridium (10.94%), Ruminococcaceae_unclassified (10.43%), Lachnospiraceae_unclassified (5.26%), Coriobacteriales_unclassified (5.89%), and Faecalibacterium (4.2%) were detected to staple the microbial flora in the CT birds. One phylum and 13 genera were found to have the significant difference between the two bird groups (p<0.05). At phylum level, Proteobacteria in CT ducks were found to be obviously lower than ducks in CC birds (p<0.05). At genus level, Escherichia-Shigella (p<0.05) and Peptococcus (p<0.05) were found to be notably lower in CT birds, while Erysipelatoclostridium (p<0.05), Ruminococcaceae_unclassified (p<0.01), Coriobacteriales_unclassified (p<0.05), Faecalibacterium (p<0.01), Atopobiaceae_unclassified (p<0.01), Alistipes (p<0.05), Eggerthellaceae_unclassified (p<0.05), Prevotella_7 (<0.05), Rikenellaceae_RC9_gut_group (p<0.05), Prevotellaceae_uncultured (p<0.05), and Shuttleworthia (p<0.05) were observed to be prominently higher in CT ducks. In conclusion, the present study revealed the effects of keeping ducks away from swimming with obvious changes in the microbial community. Though higher microbial richness was found in the ducks without swimming, more pathogenic genera including Eggerthella, Erysipelatoclostridium, Alistipes, Prevotella_7, and Shuttleworthia; zoonotic genera including Eggerthella and Shuttleworthia; inflammatory genus Alistipes; anti-inflammatory Faecalibacterium genus; and tumor genus Rikenellaceae were examined in these ducks. The CT ducks also showed significant changes at genera level regarding the metabolism (Peptococcus, Ruminococcaceae, and Coriobacteriales).

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Section: Introductionmentioning

confidence: 99%

Comparison of the Intestinal Microbial Community in Ducks Reared Differently through High-Throughput Sequencing

Zhao

Luo

et al. 2019

BioMed Research International

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“…Most notably, the relative abundance of Clostridium and Lachnospiraceae were significantly increased in tilmicosin-treated calves compared to control and BRD-affected calves (P < 0.05; Figure 2). Interestingly, it has been recently shown that an abundance of Clostridium species is associated with antibiotic-associated colitis and influenza in humans (31,32), and hemorrhagic diarrhea in feedlot cattle (15). The relative abundance of Microbacteriaceae and Turicibacter was significantly decreased in tilmicosin-treated and BRD-affected calves when compared to control calves (P < 0.05; Figure 2).…”

Section: Comparison Of Np Microbiota Across the Different Groupsmentioning

confidence: 90%

“…Extraction of DNA was performed from all NP swabs using power R Fecal DNA isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA) following the manufacturer's instructions (15). Total DNA concentration and purity were evaluated by optical density using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE, USA) at the wavelengths of 230, 260, and 280 nm, and the OD260/280 ratio of DNA ranged between 1.75 and 1.90.…”

Section: Dna Extraction 16s Rrna Gene Sequencing and Bioinformaticsmentioning

confidence: 99%

Effects of Tilmicosin Treatment on the Nasopharyngeal Microbiota of Feedlot Cattle With Respiratory Disease During the First Week of Clinical Recovery

2020

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While the nasopharyngeal (NP) microbiota is believed to be a key player in bovine respiratory health, there is limited published information about the change of NP microbiota associated with clinical recovery from bovine respiratory disease (BRD). The objective of this study was to evaluate the effect of tilmicosin treatment on the NP microbiota composition and diversity of BRD-affected calves during the first week of clinical recovery. Deep NP swabs were collected from diseased calves at the initial diagnosis of BRD, and again 7 days after the administration of a single dose of tilmicosin. As an experimental control, samples were collected from clinically healthy, pen-matched calves at the time of initial BRD diagnosis. In general, the NP microbiota from the control calves were more diverse than the NP microbiota from tilmicosin treated and BRD-affected calves. Principle coordinate analysis (PCOA) of Bray-Curtis and Jaccard dissimilarity also revealed that the overall composition of NP microbial communities in tilmicosin-treated calves closely resembled that of BRD-affected calves but differed significantly from pen-matched healthy calves. Overall, it appeared that there were only minor changes in NP microbial communities following tilmicosin treatment and, during the early phase of clinical recovery the NP microbiota in treated animals was disparate from that observed in healthy control calves. Understanding the potential impact of this prolonged recovery in mucosal microbiota would be important in optimizing the use of antimicrobials in health management programs in the feedlot industry.

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“…There is evidence that the ruminal bacterial community plays a crucial role in the regulating pathologic of organism in developing disease [29]. The role of microbial populations has received increased attention across several disciplines in recent years.…”

Section: Discussionmentioning

confidence: 99%

Characterization of bacterial community of rumen from bovine during laminitis challenge by high-throughput sequencing

Guo

Sun

Maimai

et al. 2020

Preprint

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Background: Laminitis is a classic problem in the dairy industry, which can cause a great economic loss. However, the etiology and pathogenesis of laminitis have yet to be understood. In recent years, the microbiota has been the focus of much investigation in the search for various diseases. The present study aimed to explore the relationship between ruminal bacterial microbiota and laminitis. Results: The serum of healthy and laminitis bovines (n=8, respectively) collected from farms was used to detect concentrations of LPS, lactic acid, and histamine by the detection kits. This study used 16S rRNA sequencing to identify the differences in the bacterial community. The results showed that there was a significant increase in LPS and lactic acid in the laminitis group. Furthermore, microbial data analysis revealed that the laminitis group increased the abundance of bacteria with acid metabolites, such as Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, and Erysipelotrichaceae UCG-008, and [Clostridium] papyrosolvens and Ruminococcaceae bacterium AE2021.Conclusions: In summary, the changes in ruminal bacteria may potentially serve as the risk of laminitis.

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Dysbiosis of the fecal microbiota in feedlot cattle with hemorrhagic diarrhea

Cited by 64 publications

References 32 publications

Comparison of the Intestinal Microbial Community in Ducks Reared Differently through High-Throughput Sequencing

Comparison of the Intestinal Microbial Community in Ducks Reared Differently through High-Throughput Sequencing

Effects of Tilmicosin Treatment on the Nasopharyngeal Microbiota of Feedlot Cattle With Respiratory Disease During the First Week of Clinical Recovery

Characterization of bacterial community of rumen from bovine during laminitis challenge by high-throughput sequencing

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