DyP-type Peroxidases: A Promising and Versatile Class of Enzymes

Fraaije, Marco W.

doi:10.4172/2329-6674.1000e105

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…Furthermore, MnP is capable of oxidizing Mn 2+ to Mn 3+ , which is a diffusible oxidant capable of penetrating the cell wall matrix and degrading phenolic substrates (Hofrichter 2002 ). Otherwise, the Mn(II)-independent activity of some peroxidases also allows the oxidation of different compounds with a similar structure to synthetic dyes (Fraaije 2012 ), but with the advantage of not requiring the presence of manganese, which simplifies the biodegradation process. However, the major drawbacks of these enzymes for a wide industrial application are their high production cost and their limited yield (Elisashvili and Kachlishvili 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams

González-Rodríguez

Lú-Chau

Trueba-Santiso

et al. 2022

Appl Microbiol Biotechnol

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Enzymes offer interesting features as biological catalysts for industry: high specificity, activity under mild conditions, accessibility, and environmental friendliness. Being able to produce enzymes in large quantities and having them available in a stable and reusable form reduces the production costs of any enzyme-based process. Agricultural residues have recently demonstrated their potential as substrates to produce ligninolytic enzymes by different white rot fungi. In this study, the biotechnological production of a manganese peroxidase (MnP) by Irpex lacteus was conducted through solid-state fermentation (SSF) with wheat straw as substrate and submerged fermentation (SmF) employing wheat straw extract (WSE). The obtained enzyme cocktail also showed manganese-independent activity (MiP), related to the presence of a short MnP and a dye-decolorizing peroxidase (DyP) which was confirmed by shotgun proteomic analyses. In view of the enhanced production of ligninolytic enzymes in SmF, different parameters such as WSE concentration and nitrogen source were evaluated. The highest enzyme titers were obtained with a medium formulated with glucose and peptone (339 U/L MnP and 15 U/L MiP). The scale-up to a 30 L reactor achieved similar activities, demonstrating the feasibility of enzyme production from the residual substrate at different production scales. Degradation of five emerging pollutants was performed to demonstrate the high oxidative capacity of the enzyme. Complete removal of hormones and bisphenol A was achieved in less than 1 h, whereas almost 30% degradation of carbamazepine was achieved in 24 h, which is a significant improvement compared to previous enzymatic treatments of this compound. Key points • Wheat straw extract is suitable for the growth of I. lacteus. • The enzyme cocktail obtained allows the degradation of emerging contaminants. • Mn-dependent and Mn-independent activities increases the catalytic potential. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams

González-Rodríguez

Lú-Chau

Trueba-Santiso

et al. 2022

Appl Microbiol Biotechnol

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“…Peroxidases are oxidoreductases involved in a variety of biochemical processes, including the biosynthesis of cell wall material and immunological host-defense responses [1]. The recently discovered DyP-type peroxidases (DyPs, dye-decolorizing peroxidases; EC 1.11.1.19) represent a novel superfamily of heme-containing enzymes.…”

Section: Introductionmentioning

confidence: 99%

Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1

et al. 2019

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The consumption of dyes is increasing worldwide in line with the increase of population and demand for clothes and other colored products. However, the efficiency of dyeing processes is still poor and results in large amounts of colored effluents. It is desired to develop a portfolio of enzymes which can be used for the treatment of colored wastewaters. Herein, we used genome sequence information to discover a dye-decolorizing peroxidase (DyP) from Pseudomonas fluorescens Pf-01. Two genes putatively encoding for DyPs were identified in the respective genome and cloned for expression in Escherichia coli, of which one (PfDyP B2) could be overexpressed as a soluble protein. PfDyP B2 shows some typical features known for DyPs which includes the ability to convert dyes at the expense of hydrogen peroxide. Interestingly, t-butyl hydroperoxide could be used as an alternative substrate to hydrogen peroxide. Immobilization of PfDyP B2 in calcium-alginate beads resulted in a significant increase in stability: PfDyP B2 retains 80% of its initial activity after 2 h incubation at 50 °C, while the soluble enzyme is inactivated within minutes. PfDyP B2 was also tested with aniline and ethyl diazoacetate as substrates. Based on GC-MS analyses, 30% conversion of the starting material was achieved after 65 h at 30 °C. Importantly, this is the first report of a DyP-catalyzed insertion of a carbene into an N-H bond.

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“…The lignin degradation process is further supported by other extracellular accessory enzymes, which include glucose oxidase (GLOX; EC 1.1.3.4), glyoxylate oxidase (EC 1.2.3.5), aryl-alcohol oxidase (AAO; EC 1.1.3.7), aryl-alcohol dehydrogenase (AAD; EC 1.1.1.90), and quinone reductases (EC 1.6.5.5) (Wong 2009;Dashtban et al 2010;Fraaije and van Bloois 2012;Pollegioni et al 2015;Stajić et al 2016;Falade et al 2017;Ceballos 2018).…”

Section: Lignin and Lignin-modifying Enzymesmentioning

confidence: 99%

Unlocking proteomic and ecological heterogeneity between Schizophyllum commune and Trametes versicolor as early sapwood colonizers on wood

Zia¹

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Pilze sind ein wesentlicher Bestandteil von Waldökosystemen und holzzerstörende Pilze spielen eine Schlüsselrolle beim Abbau von Holz und beim Nährstoffrecycling. Der vollständige Abbau von Holz durch eine einzelne Pilzart ist aufgrund der widerspenstigen physikalischen Struktur und chemischen Zusammensetzung von Holz eine Herausforderung. In der Natur ist verrottendes Holz daher ein Unterschlupf für mehrere holzzersetzende Pilze. Diese Pilze sind neben anderen die Hauptakteure im Holzzerfallsprozess. Bestimmte holzzerstörende Pilze besiedeln früh das Holz und starten den Zerfallsprozess. In diesem Zusammenhang werden zwei frühe Splintholzbesiedler, Schizophyllum commune und Trametes versicolor, oft gemeinsam in Form von Pilzen auf Ästen lebender Bäume, auf frisch gefallenem Totholz und auch auf geschnittenen Stämmen gefunden. In früheren Berichten und in eigenen Beobachtungen erschienen die Fruchtkörper beider Pilze zuerst in der frühen Fäulnisphase auf dem morschen Holz. Allerdings ist S. commune ein schwacher Zersetzer mit Merkmalen zwischen Weiß-und Braunfäule (Graufäule) und könnte Vorteile von mitbewohnenden Pilzen haben, die besser in der Lage sind, Holz abzubauen. T. versicolor hingegen ist eine aggressive Weißfäule. Zwischen diesen beiden frühen Splintholzbesiedlern bestehen Interaktionen, die sowohl kompetitiv als auch kooperativ sein können. In dieser Arbeit ging es darum, die Interaktionen zwischen den frühen Splintholzbesiedlern (S. commune und T. versicolor) in Laborexperimenten in Doppelkulturen auf Buchenholz besser zu verstehen und die Sekretome/Proteome aus Einzel-und Doppelkulturen, die auf Buchensplintholz oder künstlichem Substrat gewachsen sind, zu isolieren, um Funktionen beim Wachstum auf Holz, bei der Entgiftung schädlicher Holzinhaltsstoffe und bei der Verteidigung gegen andere Pilze zu definieren. Buchen-Splintholz-und Rindenfäulnisversuche wurden durchgeführt, um die Fäulniskapazität von zwei frühen Splintholzbesiedlern, S. commune und T. versicolor, unter verschiedenen Beleuchtungsschemata zu testen und zu vergleichen. Drei Stämme von S. commune (Dikaryon Sc-D; die beiden elterlichen Monokaryonen Sc-M1 und Sc-M2) und zwei Stämme von T. versicolor (Dikaryon Tv-D; Monokaryon Tv-M) wurden auf Buchenpartikel (ca. 4 x 1 mm) inokuliert und bei 25 o C unter verschiedenen Lichtbedingungen (in kontinuierlicher Dunkelheit -CD, in Dunkelheit -D, in kontinuierlichem Licht -CL, und in 12 h Dunkelheit/12 h Licht-Zyklen -12L/12D) für 30 und 90 Tage bebrütet. Das aggressive Verhalten von T. versicolor wurde durch XIV | P a g e Tests an Buchensplintholz und Rindenfäule verifiziert. Insgesamt verursachte T. versicolor eine höhere Reduktion des Rinden-und Splintholzgewichts als S. commune. Im Splintholz reduzierte T. versicolor das Gewicht innerhalb von 90 Tagen um maximal 53 % (Tv-D) und 24 % (Tv-M), während S. commune den Gewichtsverlust in der gleichen Zeit nur bis zu 6 % erreichte (Sc-D: 4 %; Sc-M1: 5 % und Sc-M2: 6 %). Alle Stämme von S. commune reduzierten unter allen Licht-und Dunkelbedingungen n...

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DyP-type Peroxidases: A Promising and Versatile Class of Enzymes

Cited by 6 publications

References 34 publications

Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams

Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams

Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1

Unlocking proteomic and ecological heterogeneity between Schizophyllum commune and Trametes versicolor as early sapwood colonizers on wood

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