Dynorphin-A immunoreactive terminals on the neuronal somata of rat mesencephalic trigeminal nucleus

Yamada, Kentaro; Park, H.; Sato, Sadao; Onozuka, Minoru; Kubo, Kin‐ya; Yamamoto, Toshiharu

doi:10.1016/j.neulet.2008.04.030

Cited by 20 publications

(9 citation statements)

References 32 publications

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“…After washing in PB and immersion in 20% sucrose, samples were cut into 10-um-thick transverse sections using a sliding microtome equipped with a frozen stage and sections were immunostained by the free-floating method. Immunohistochemistry was performed according to our routine method (Yamada et al, 2008). Briefly, the sections were washed overnight in 0.1 M PB containing 0.9% NaCl and incubated with rabbit polyclonal antibodies against human GR (Abcam, CA, USA) diluted 1:100 in PBS containing 1% bovine serum albumin (BSA) for 24 h at 4 1C.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Genetic regulatory network analysis reveals that low density lipoprotein receptor-related protein 11 is involved in stress responses in mice

Cai

et al. 2014

Psychiatry Research

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Section: Immunohistochemistrymentioning

confidence: 99%

Genetic regulatory network analysis reveals that low density lipoprotein receptor-related protein 11 is involved in stress responses in mice

Cai

et al. 2014

Psychiatry Research

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“…Tissue was mounted on gelatin‐subbed slides and coverslipped with SlowFade medium (Thermo Fisher Scientific, Waltham, MA, USA). All antibodies had previously been characterised and validated by preadsorption experiments (Table ) . However, only the AgRP and kisspeptin antibodies had previously been validated in nonhuman primate tissue.…”

Section: Methodsmentioning

confidence: 99%

“…All antibodies had previously been characterised and validated by preadsorption experiments ( Table 1). [33][34][35][36][37][38][39]…”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

Arcuate nucleus neuropeptide coexpression and connections to gonadotrophin‐releasing hormone neurones in the female rhesus macaque

True

Takahashi

Kirigiti

et al. 2017

J Neuroendocrinology

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The underlying hypothalamic neurocircuitry by which metabolism and feeding regulates reproductive function has been well-studied in the rodent; however, recent data demonstrated significant neuroanatomical differences in the human brain. The current study had three objectives, centered on arcuate nucleus neuropeptides regulating feeding and reproduction: 1) characterize coexpression patterns in the female nonhuman primate, 2) establish whether these neuronal populations make potential contacts with GnRH neurons and 3) determine whether these contacts differ between the low and high GnRH-releasing states of pre-puberty and adulthood, respectively. Female nonhuman primates have several coexpression patterns of hypothalamic neuropeptides that differ from those reported in rodents. Cocaine- and amphetamine-regulated transcript (CART) is not coexpressed with proopiomelanocortin (POMC) but instead with neuropeptide Y (NPY). CART is also expressed in a subpopulation of kisspeptin cells in the nonhuman primate, similar to observations in humans but diverging from findings in rodents. Very few GnRH-expressing neurons received close appositions from double-labeled kisspeptin/CART fibers; however, both single-labeled kisspeptin and CART fibers were in frequent apposition with GnRH neurons with no differences between prepubertal and adult animals. NPY/AgRP coexpressing fibers contacted significantly more GnRH neurons in prepubertal animals than adults, consistent with increased NPY and AgRP mRNA observed in prepubertal animals. The current findings detail significant differences in arcuate nucleus neuropeptide coexpression in the monkey compared to the rodent and are consistent with the hypothesis that arcuate nucleus NPY/AgRP neurons play an inhibitory role in controlling GnRH neuronal regulation in the prepubertal primate.

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“…For immunohistochemistry [21], the animals were deeply anesthetized using pentobarbital sodium (35 mg/kg i.p., Wako Pure Chemical Industries Ltd., Osaka, Japan), and were perfused transcardially with 0.9% NaCl, and then with 4% paraformaldehyde and 0.2% picric acid in 0.1 M sodium phosphate buffer (PB, pH 6.9). The brain was rapidly removed by dissection, blocked into three parts (cut transversely at the rostral end of the superior colliculus and caudal end of the inferior colliculus), and further fixed for 1 or 2 days at 4 • C in the same fixative.…”

Section: Methodsmentioning

confidence: 99%

Chewing suppresses the stress-induced increase in the number of pERK-immunoreactive cells in the periaqueductal grey

Yamada

Narimatsu

Ono

et al. 2015

Neuroscience Letters

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We investigated the effects of chewing under immobilization stress on the periaqueductal gray (PAG) matter using phosphorylated extracellular signal-regulated kinase (pERK) as a marker of responding cells. Immobilization stress increased pERK-immunoreactive cells in the PAG. Among four subdivisions of the PAG, the increase of immunoreactive cells was remarkable in the dorsolateral and ventrolateral subdivisions. However, increase of pERK-immunoreactive cells by the immobilization stress was not so evident in the dorsomedial and lateral subdivisions. The chewing under immobilization stress prevented the stress-induced increase of pERK-immunoreactive cells in the dorsolateral and ventrolateral subdivisions with statistical significances (p<0.05). Again, chewing effects on pERK-immunoreactive cells were not visible in the dorsomedial and lateral subdivisions. These results suggest that the chewing alleviates the PAG (dorsolateral and ventrolateral subdivisions) responses to stress.

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Dynorphin-A immunoreactive terminals on the neuronal somata of rat mesencephalic trigeminal nucleus

Cited by 20 publications

References 32 publications

Genetic regulatory network analysis reveals that low density lipoprotein receptor-related protein 11 is involved in stress responses in mice

Genetic regulatory network analysis reveals that low density lipoprotein receptor-related protein 11 is involved in stress responses in mice

Arcuate nucleus neuropeptide coexpression and connections to gonadotrophin‐releasing hormone neurones in the female rhesus macaque

Chewing suppresses the stress-induced increase in the number of pERK-immunoreactive cells in the periaqueductal grey

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