Dyneins

Cypranowska, C.A.; Yıldız, Ahmet; Ishikawa, Takuji

doi:10.1016/b978-0-12-394447-4.20101-6

Cited by 2 publications

(3 citation statements)

References 240 publications

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“…In zebra sh, endocardial primary cilia of the heart, important mediators of uid ow, play an important role in early embryonic development [43]. Although dnali1 was less expressed in the ounder heart than in the testis, it was required for the motility of cilia and agella [3,7] and might have function in the ounder heart. It has been proved that 3'UTR of dnali1 not only regulates mRNA-based processes, such as mRNA localization, mRNA stability, and translation, but also transmits genetic information encoded in 3′UTRs to proteins through the establishment of its mediated protein-protein interaction [44].…”

Section: Discussionmentioning

confidence: 99%

“…Cilia are also involved in developmental processes, including left-right asymmetry formation, limb morphogenesis, and the patterning of neurons in the neural tube [6]. Dynein light and intermediate chains are required for the motility of dynein heavy chain and the assembly of axonemal dynein into cilia and agella [3,7]. Dynein axonemal light and intermediate chain1 gene (dnali1) encodes one light-intermediate chain and contains a coiled-coil domain in C-terminal region.…”

Section: Introductionmentioning

confidence: 99%

“…Dyneins, a family of major cytoskeletal motors [1], have wide variety of cellular functions [2] and can be divided into two types, axonemal and cytoplasmic dyneins [3]. They consist of heavy, intermediate, light intermediate, and light chains with different molecular weight and function [4].…”

Section: Introductionmentioning

confidence: 99%

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Sexually Dimorphic Expression and Special Regulatory Sequence of Dnali1 in the Olive Flounder Paralichthys Olivaceus

Tan

Zou

Wang

et al. 2021

Preprint

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Dynein axonemal light intermediate chain 1 (dnali1) is an important part of axonemal dyneins and plays an important role in the growth and development of animals. However, there is little information about dnali1 in fish. Herein, we cloned dnali1 gene from the genome of olive flounder (Paralichthys olivaceus), a commercially important maricultured fish in China, Japan, and Korea, and analyzed its expression patterns in different gender fish. The DNA sequence of the entire gene contained a 771 bp open reading frame (ORF), two different sizes of 5' untranslated region (5'UTR), and a 1499 bp 3' untranslated region (3'UTR). Two duplicated 922 nt fragments were found in dnali1 mRNA. The first fragment contained the downstream coding region and the front portion of 3'UTR, and the second fragment was entirely located in 3’UTR. Multiple alignments indicated that the flounder Dnali1 protein contained the putative conserved domain. Its expression showed sexually dimorphic with predominant expression in the flounder testis, and lower expression in other tissues. The gene with the larger 5’UTR was specifically expressed in the testis. The highest expression level in the testis was detected at stages Ⅳ and Ⅴ. Transient expression analysis showed that the 922 bp repeated sequence 3’UTR of dnali1 down-regulated the expression of GFP at the early stage in zebrafish. The results implied that dnali1 might play an important role in flounder testis, especially in the period of spermatogenesis, and the 5’UTR and the repetitive sequences in 3’UTR might contain some special regulatory elements for the cilia.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sexually Dimorphic Expression and Special Regulatory Sequence of Dnali1 in the Olive Flounder Paralichthys Olivaceus

Tan

Zou

Wang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus

et al. 2021

View full text Add to dashboard Cite

Dynein axonemal light intermediate chain 1 (dnali1) is an important part of axonemal dyneins and plays an important role in the growth and development of animals. However, there is little information about dnali1 in sh. Herein, we cloned dnali1 gene from the genome of olive ounder (Paralichthys olivaceus), a commercially important maricultured sh in China, Japan, and Korea, and analyzed its expression patterns in different gender sh. The DNA sequence of the entire gene contained a 771 bp open reading frame (ORF), two different sizes of 5' untranslated region (5'UTR), and a 1499 bp 3' untranslated region (3'UTR). Two duplicated 922 nt fragments were found in dnali1 mRNA. The rst fragment contained the downstream coding region and the front portion of 3'UTR, and the second fragment was entirely located in 3'UTR. Multiple alignments indicated that the ounder Dnali1 protein contained the putative conserved domain. Its expression showed sexually dimorphic with predominant expression in the ounder testis, and lower expression in other tissues. The gene with the larger 5'UTR was speci cally expressed in the testis. The highest expression level in the testis was detected at stages and . Transient expression analysis showed that the 922 bp repeated sequence 3'UTR of dnali1 down-regulated the expression of GFP at the early stage in zebra sh. The results implied that dnali1 might play an important role in ounder testis, especially in the period of spermatogenesis, and the 5'UTR and the repetitive sequences in 3'UTR might contain some special regulatory elements for the cilia.

show abstract

Dyneins

Cited by 2 publications

References 240 publications

Sexually Dimorphic Expression and Special Regulatory Sequence of Dnali1 in the Olive Flounder Paralichthys Olivaceus

Sexually Dimorphic Expression and Special Regulatory Sequence of Dnali1 in the Olive Flounder Paralichthys Olivaceus

Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus

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