Dynamics of Wolbachia Populations in Transfected Lines of Trichogramma

Pintureau, Bernard; Grenier, S.; Boléat, Bénédicte; Lassablière, François; Heddi, Abdelaziz; Khatchadourian, Chaqué

doi:10.1006/jipa.2000.4953

Cited by 28 publications

(26 citation statements)

References 22 publications

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“…Concentration of Wolbachia in the germ plasm of embryos has been reported previously for the Drosophila melanogaster Canton-S strain (16), the wasp Nasonia (4), some Trichogramma species (32,40), and Aphytis (47). On the other hand, Wolbachia cells were found to be equally distributed in the cortex of Drosophila simulans Riverside embryos (3,31).…”

supporting

confidence: 61%

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Heads or Tails: Host-Parasite Interactions in the Drosophila-Wolbachia System

Veneti

Clark

Karr

et al. 2004

Appl Environ Microbiol

146

164

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Wolbachia strains are endosymbiotic bacteria typically found in the reproductive tracts of arthropods. These bacteria manipulate host reproduction to ensure maternal transmission. They are usually transmitted vertically, so it has been predicted that they have evolved a mechanism to target the host's germ cells during development. Through cytological analysis we found that Wolbachia strains display various affinities for the germ line of Drosophila. Different Wolbachia strains show posterior, anterior, or cortical localization in Drosophila embryos, and this localization is congruent with the classification of the organisms based on the wsp (Wolbachia surface protein) gene sequence. This embryonic distribution pattern is established during early oogenesis and does not change until late stages of embryogenesis. The posterior and anterior localization of Wolbachia resembles that of oskar and bicoid mRNAs, respectively, which define the anterior-posterior axis in the Drosophila oocyte. By comparing the properties of a single Wolbachia strain in different host backgrounds and the properties of different Wolbachia strains in the same host background, we concluded that bacterial factors determine distribution, while bacterial density seems to be limited by the host. Possible implications concerning cytoplasmic incompatibility and evolution of strains are discussed.

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supporting

confidence: 61%

“…In Trichogramma, posterior localization of Wolbachia has also been described. However, when transferred to a naturally uninfected line, Wolbachia did not have a similar posterior localization (32). In addition, with the transinfected line there were successively decreasing numbers of bacteria, which led to loss of infection.…”

Section: Resultsmentioning

confidence: 92%

Heads or Tails: Host-Parasite Interactions in the Drosophila-Wolbachia System

Veneti

Clark

Karr

et al. 2004

Appl Environ Microbiol

146

164

View full text Add to dashboard Cite

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“…Wolbachia host cell range have consisted of interspecific transinfection attempts (5,6,17,18,20,28). However, interpretation of these prior experiments has been complicated by technical problems associated with embryonic microinjection.…”

Section: Conclusion Prior Investigations Ofmentioning

confidence: 99%

Characterization ofWolbachiaHost Cell Range via the In Vitro Establishment of Infections

Dobson

Marsland

Veneti

et al. 2002

Appl Environ Microbiol

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Maternally transmitted bacteria of the genus Wolbachia are obligate, intracellular symbionts that are frequently found in insects and cause a diverse array of reproductive manipulations, including cytoplasmic incompatibility, male killing, parthenogenesis, and feminization. Despite the existence of a broad range of scientific interest, many aspects of Wolbachia research have been limited to laboratories with insect-rearing facilities. The inability to culture these bacteria outside of the invertebrate host has also led to the existing bias of Wolbachia research toward infections that occur in host insects that are easily reared. Here, we demonstrate that Wolbachia infections can be simply established, stably maintained, and cryogenically stored in vitro using standard tissue culture techniques. We have examined Wolbachia host range by introducing different Wolbachia types into a single tissue culture. The results show that an Aedes albopictus (Diptera: Culicidae) cell line can support five different Wolbachia infection types derived from Drosophila simulans (Diptera: Drosophilidae), Culex pipiens (Culicidae), and Cadra cautella (Lepidoptera: Phycitidae). These bacterial types include infection types that have been assigned to two of the major Wolbachia clades. As an additional examination of Wolbachia host cell range, we demonstrated that a Wolbachia strain from D. simulans could be established in host insect cell lines derived from A. albopictus, Spodoptera frugiperda (Lepidoptera: Noctuidae), and Drosophila melanogaster. These results will facilitate the development of a Wolbachia stock center, permitting novel approaches for the study of Wolbachia infections and encouraging Wolbachia research in additional laboratories.

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“…However, stable transovarial transmission of the infection in lineages after transfer has been more difficult to achieve. Failures often occurred when Wolbachia have been transfected into a host phylogenetically distant from their native host (Rigaud and Juchault, 1995;Van Meer and Stouthamer, 1999;Heath et al, 1999;Pintureau et al, 2000), whereas successes were generally found between closely related species (Boyle et al, 1993;Braig et al, 1994;Clancy and Hoffmann, 1997). Only a few attempts at horizontal transfer of Wolbachia have led to permanent establishment in a new host species (Breeuwer and Werren, 1990;Clancy and Hoffmann, 1997;Pintureau et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Superinfection of Laodelphax striatellus with Wolbachia from Drosophila simulans

Kang

Cai

et al. 2003

Heredity

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Wolbachia are maternally inherited, intracellular a-proteobacteria that infect a wide range of arthropods. They manipulate the reproduction of hosts to facilitate their spread into host populations, through ways such as cytoplasmic incompatibility (CI), parthenogenesis, feminization and male killing. The influence of Wolbachia infection on host populations has attracted considerable interest in their possible role in speciation and as a potential agent of biological control. In this study, we used both microinjection and nested PCR to show that the Wolbachia naturally infecting Drosophila simulans can be transferred into a naturally Wolbachiainfected strain of the small brown planthopper Laodelphax striatellus, with up to 30% superinfection frequency in the F 12 generation. The superinfected males of L. striatellus showed unidirectional CI when mated with the original single-infected females, while superinfected females of L. striatellus were compatible with superinfected or single-infected males. These results are, to our knowledge, the first to establish a superinfected horizontal transfer route for Wolbachia between phylogenetically distant insects. The segregation of Wolbachia from superinfected L. striatellus was observed during the spreading process, which suggests that Wolbachia could adapt to a phylogenetically distant host with increased infection frequency in the new host population; however, it would take a long time to establish a highfrequency superinfection line. This study implies a novel way to generate insect lines capable of driving desired genes into Wolbachia-infected populations to start population replacement.

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Dynamics of Wolbachia Populations in Transfected Lines of Trichogramma

Cited by 28 publications

References 22 publications

Heads or Tails: Host-Parasite Interactions in the Drosophila-Wolbachia System

Heads or Tails: Host-Parasite Interactions in the Drosophila-Wolbachia System

Characterization ofWolbachiaHost Cell Range via the In Vitro Establishment of Infections

Superinfection of Laodelphax striatellus with Wolbachia from Drosophila simulans

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