Dynamics of the IgG3 responses following immunisation of BALB/c mice with somatic and excretory/secretory antigens from various Trichinella species

Abstract

Abstract.Comparison of the dynamics and antigen recognition profiles of IgG3 following immunisation with larval crude extracts (LCE) and excretory-secretory (ES) products from muscle larvae of different species of Trichinella (T. spiralis, T. nativa, T. britovi, T. nelsoni and genotype T6) was made in BALB/c mice. High levels of IgG3 response were obtained in ELISA following immunisation with LCE from all species with maximum levels achieved between days 59 and 64 post-immunisation (p.i.) and maintained up to the limit of the observation (day 164). Antigen recognition profiles as measured by western-blot showed dense and numerous bands in the range 45-64 kDa that were consistent from week 5 th with variation in epitope recognition among the different species. Following immunisation with ES antigens a significant decrease in IgG3 response was observed for all species especially for T. nativa in comparison to LCE. Antigen recognition on ES antigens showed three main bands in the range of 45-60 kDa for all species excepting T. nelsoni and T. britovi where an additional band was also present. These results clearly show that IgG3 epitopes are more abundant in somatic (LCE) than in ES products of Trichinella muscle larvae and that quantitative as well as qualitative variations exist among different species.

Comparative analysis of excretory-secretory antigens of Trichinella spiralis and Trichinella britovi muscle larvae by two-dimensional difference gel electrophoresis and immunoblotting

“…These findings were supported by another study that demonstrated a similar antigenic pattern for the T. spiralis E-S proteins, with three major protein bands migrating between 40-60 kDa that showed high cross-reactivity [54]. In our study, the antigenic proteins had a lower molecular mass, which could be a result of the methods used for proteome analyses and/or individual variations between the antisera used in the present and in the published studies.…”

“…In the case of T. britovi , 2-D immunoblotting revealed two groups of horizontally adjacent antigenic spots (Figure 2B ). Previous 1-DE and 2-DE immunoblotting studies involving the crude extract isolated from T. britovi and Trichinella genotype 8 ML revealed that the major antigens are acidic with molecular mass values ranging from 45 to 70 kDa [ 35 , 54 ]. These highly cross-reactive proteins of T. spiralis and T. britovi have been identified as glycoproteins with a postulated role in infection and nurse cell development [ 55 , 56 ].…”

A Study of Immunotherapeutic Efficacy of Trichinella Spiralis Excretory-Secretory Proteins in Murine Trichinellosis

“…The protective effect was previously evaluated by many authors, but the therapeutic effect was no used as a therapeutic agent. Crude ES antigens were tested as a vaccine either alone or with adjuvant by many authors (Robinson et al, 1995a;Dea-Ayuela and Bolas-Fernández, 2000;Quan et al, 2004). These authors reported that immunization might elicit an effective immune response, resulting in complete protection against infective larvae.…”

“…GI: Non-infected, non-vaccinated and nontreated mice, GII: Infected non-vacciated and non-treated mice, each one received 300 T. spiralis infective larvae. GIII: Vaccinated with AW/ES antigen, 100µg in PBS injected subcutaneously 7 days before oral infection with 300 T. spiralis muscle larvae (Dea-Ayuela and Bolas-Fernández, 2000). GIV: Vaccinated with larval ES antigen 100µg in PBS injected subcutanously 7 days before oral infection with 300 T. spiralis muscle larvae.…”

Trichinella spiralis Calreticulin Binds Human Complement C1q As an Immune Evasion Strategy

“…Crude somatic extracts of all the stages of T . spiralis were prepared by homogenizing the worms based on conventional methods (28). The excretory–secretory products of ML (MES) and adult worms (AES) were collected using previously described culture methods (29, 30).…”