Dynamics of the bacterial community structure in the rhizosphere of a maize cultivar

Li, Xiangzhen; Rui, Junpeng; Mao, Yuejian; Yannarell, Anthony C.; Mackie, Roderick I.

doi:10.1016/j.soilbio.2013.10.017

Cited by 306 publications

(187 citation statements)

References 40 publications

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“…In order to minimize PCR bias, two PCR reactions were set up for each sample, and the PCR products in the replicate reactions were pooled. The details in PCR and related procedures were described previously (Li et al 2014). The amplicons from each sample were pooled with an equimolar concentration and pyrosequenced with the GS FLX+ system (454 Life Sciences, Branford, CT).…”

Section: Analytic Methodsmentioning

confidence: 99%

Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure

Rui

Pei

et al. 2014

Appl Microbiol Biotechnol

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Anaerobic co-fermentation of straw and manure is widely used for waste treatment and biogas production. However, the differences between the straw-and slurry-associated prokaryotic communities, their dynamic changes throughout the co-fermentation process, and their correlations with bioreactor performance are not fully understood. To address these questions, we investigated the prokaryotic community compositions and the dynamics of prokaryotes attached to the straw and in the slurry during co-fermentation of wheat straw and swine manure using pyrosequencing technique. The results showed that straw-and slurry-associated prokaryotes were different in their structure and function. Strawassociated prokaryotic communities were overrepresented by the phyla Spirochaetes and Fibrobacteres, while Synergistetes and Euryarchaeota were more abundant in the slurry. The straw-associated candidate class TG3, genera Fibrobacter, Bacteroides, Acetivibrio, Clostridium III, Papillibacter, Treponema, Sedimentibacter, and Lutispora may specialize in substrate hydrolysis. Propionate was the most abundant volatile fatty acid in the slurry, and it was probably degraded through syntrophic oxidation by the genera Pelotomaculum, Methanoculleus, and Methanosaeta. The protein-fermenting bacteria Aminobacterium and Cloacibacillus were much abundant in the slurry, indicating that proteins are important substrates in the co-fermentation. This study provided a better understanding of the anaerobic co-fermentation process that is driven by spatially differentiated microbiota.

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Section: Analytic Methodsmentioning

confidence: 99%

Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure

Rui

Pei

et al. 2014

Appl Microbiol Biotechnol

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“…Two parallel 25 lL PCR reactions were conducted, and the PCR products were pooled for purification using electrophoresis. The details concerning PCR procedure and sample preparation were described before (Li et al, 2014b). In total, 60 samples were prepared for sequencing using the Illumina Miseq platform at Environmental Genome Platform of Chengdu Institute of Biology.…”

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Temperature affects microbial abundance, activity and interactions in anaerobic digestion

Lin

Vrieze

et al. 2016

Bioresource Technology

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“…In other crops, they have been identified as tolerance inductors in wheat (Triticum sativum) and legumes (Vigna radiata) when they were inoculated (Rai et al, 2007), mientras que mediante pyrosecuenciación se identificó como los más abundantes en la rizosfera de maíz a los phyla Proteobacteria, Bacteroidetes y Actinobacteria (Li et al, 2014).…”

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“…(Ikeda et al, 2013). By analyzing these populations using gas chromatography and fatty acid profiles, Bacillus pumilus, B. subtilis, Pseudomonas aeruginosa and P. fluorescens were identified as the most predominant species in maize stalks (Rai et al, 2007), but when using pyrosequencing, the Proteobacteria, Bacteroidetes and Actinobacteria phyla were identified as being the most abundant in the maize rhizosphere (Li et al, 2014).Maize research has shown that these populations could be used as bacterial inoculants for controlling drought stress, and suggests that drought tolerance is induced by the production of phytohormones such as abscisic, gibberellic and indole-3-acetic acids, cytokinins, enzymes such as ACC deaminase, production of bacterial exopolysaccharides and systemic tolerance induction (Dimkpa et al, 2009;Kim et al, 2012; Timmusk et al, 2014;Yang et al, 2009). In other crops, they have been identified as tolerance inductors in wheat (Triticum sativum) and legumes (Vigna radiata) when they were inoculated (Rai et al, 2007), mientras que mediante pyrosecuenciación se identificó como los más abundantes en la rizosfera de maíz a los phyla Proteobacteria, Bacteroidetes y Actinobacteria (Li et al, 2014).…”

mentioning

confidence: 99%

“…By analyzing these populations using gas chromatography and fatty acid profiles, Bacillus pumilus, B. subtilis, Pseudomonas aeruginosa and P. fluorescens were identified as the most predominant species in maize stalks (Rai et al, 2007), but when using pyrosequencing, the Proteobacteria, Bacteroidetes and Actinobacteria phyla were identified as being the most abundant in the maize rhizosphere (Li et al, 2014).…”

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Bacterias endófitas de la raíz en líneas de maíces tolerantes y susceptibles a sequía

Sánchez-Bautista

Alba

Aranda-Ocampo

et al. 2018

RMF

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Resumen. El maíz (Zea mays) ocupa el segundo lugar como alimento en el mundo y la sequía limita su productividad. Las plantas albergan bacterias endófitas que influyen en la sanidad y tolerancia a la sequía. El objetivo de esta investigación fue estimar la densidad y diversidad de las bacterias endófitas cultivables de la raíz en siete líneas homocigóticas de maíces tolerantes y siete susceptibles a sequía en tres localidades de México durante tres ciclos del cultivo. La densidad y diversidad de las Root endophyte bacteria in drought-tolerant and drought-susceptible maize lines Abstract. Maize (Zea mays) ranks second as foodin the world and drought limits its productivity. Plants harbor endophytic bacteria that influence health and drought tolerance. The goal of this research was to estimate the density and diversity of cultivable endophyte bacteria from the root system of seven homozygous maize drought-tolerant and seven drought-susceptible lines in three locations of Mexico during three crop cycles. The density and diversity of bacterial populations was assessed by direct counting on plates and identified by PCR. The results identified three groups of endophytic bacteria: 1) highly frequent (Bacillus subtilis, Bacillus megaterium y Pseudomonas geniculata), 2) frequent (Bacillus firmus, Pseudomonas hibiscola y Sinorhizobium meliloti) y 3) low frequency (Acinetobacter soli, Stenotrophomonas maltophila y Burkholderia gladioli. The analysis of variance (ANOVA) showed significant differences (p≤0.05) in density ( Log10 CFU g -1 root) of population by location, crop cycle, days after sowing and Publicación en línea, enero 2018 36Fully Bilingual revista Mexicana de FitoPatoloGía Mexican Journal oF PhytoPatholoGy poblaciones bacterianas se evaluó mediante conteo directo en placas y se identificaron por PCR. Los resultados identificaron tres grupos de bacterias endófitas: 1) altamente frecuentes (Bacillus subtilis, Bacillus megaterium y Pseudomonas geniculata), 2) frecuentes (Bacillus firmus, Pseudomonas hibiscola y Sinorhizobium meliloti) y 3) baja frecuencia (Acinetobacter soli, Stenotrophomonas maltophila y Burkholderia gladioli. El análisis de varianza (ANOVA) mostró diferencias significativas (p≤0,05) en la densidad ( Log10 UFC g -1 de raíz) de población por localidad, ciclo de cultivo, días después de siembra y líneas de maíz. La densidad de Bacillus subtilis, Pseudomonas hibiscola en la localidad de El Batán y Bacillus megaterium, Sinorhizobium meliloti en Tlaltizapán, fueron significativamente mayores en las líneas de maíz tolerantes que en las susceptibles a sequía.Palabras clave: Zea mays; bacterias endofíticas; diversidad bacteriana; 16S rADN.El maíz (Zea mays) es actualmente el segundo cultivo alimenticio más importante en términos de fuentes de energía y un contenido de proteína de aproximadamente 9.2% en la nutrición humana (FAO, 2016). México ocupa el séptimo lugar en producción del cereal con aproximadamente 25 millones de toneladas al año (FIRA, 2016). La productividad de esta gramínea se limita por f...

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Dynamics of the bacterial community structure in the rhizosphere of a maize cultivar

Cited by 306 publications

References 40 publications

Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure

Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure

Temperature affects microbial abundance, activity and interactions in anaerobic digestion

Bacterias endófitas de la raíz en líneas de maíces tolerantes y susceptibles a sequía

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