Dynamics of Testicular Germ Cell Proliferation in Normal Mice and Transgenic Mice Overexpressing Rat Androgen-Binding Protein: A Flow Cytometric Evaluation1

Jeyaraj, D. A.; Grossman, Gail; Weaver, Catharina M.; Petrusz, Peter

doi:10.1095/biolreprod66.4.877

Cited by 45 publications

(51 citation statements)

References 43 publications

(67 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…expression in the testis also alter the expression of ABP (Tirado et al 2004). Altered expression of ABP has been associated with testis and male tract alterations similar to those caused by alterations of AR (Danzo et al 1977, Jeyaraj et al 2002. Therefore, it would be possible that ABP could have some effect on AR expression.…”

Section: Discussionmentioning

confidence: 99%

Differential hormonal regulation of estrogen receptors ERα and ERβ and androgen receptor expression in rat efferent ductules

et al. 2004

View full text Add to dashboard Cite

Estrogen receptors, in addition to the androgen receptor (AR), are expressed at high levels in efferent ductules of the male reproductive tract and it is now well recognized that estrogen receptor (ER) a is required for the maintenance of normal structure and function of the ductules. However, little is known regarding the hormonal regulation of the receptors themselves in the male. In the present study, efferent ductule ligation and castration, followed by replacement with testosterone, dihydrotestosterone (DHT) or estradiol was used to investigate the relative importance of circulating and luminal sources of steroid for the modulation of ERa, ERb and AR in rat efferent ductules. Uni-or bilateral castration and ligation did not affect the expression of ERa and ERb, but bilateral castration caused down-regulation of AR. Replacement with DHT and testosterone alone or in combination with estradiol caused the recovery of AR expression to control levels. A slight recovery of AR was also observed after estrogen replacement. ERa expression was decreased to nearly undetectable levels after estrogen replacement. On the other hand, ERb did not show evident effects following any of the treatments, suggesting a constitutive expression of this receptor. This differential modulation of the steroid hormone receptors highlights the importance of maintaining a physiological androgen-estrogen balance to regulate the structure and function of efferent ductules in the male.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential hormonal regulation of estrogen receptors ERα and ERβ and androgen receptor expression in rat efferent ductules

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Testicular GC suspension were isolated and cultured following the methods described by Jeyaraj et al (Jeyaraj et al, 2002) and Sette et al (Sette et al, 1999). Isolated cells were either fixed (70% cold methanol) or cultured overnight (MEM, 0.5% BSA, 1 mM sodium pyruvate and 2 mM sodium lactate; 32°C).…”

Section: Isolation Cell Culture and Treatments Of Germ Cellsmentioning

confidence: 99%

Targeted inactivation of nuclear interaction partner of ALK disrupts meiotic prophase

et al. 2012

View full text Add to dashboard Cite

SUMMARYNIPA (nuclear interaction partner of ALK) is an F-box-like protein that monitors the timing of mitotic entry. Constitutively active NIPA delays mitotic entry by preventing accumulation of nuclear cyclin B1. Here, we have investigated the consequences of Nipa inactivation by using a conditional knockout strategy. Nipa-deficient animals are viable but show a lower birth rate and reduced body weight. Furthermore, Nipa-deficient males are sterile owing to a block of spermatogenesis during meiotic prophase. Whereas Nipa -/-mouse embryonic fibroblasts show no severe phenotype, Nipa -/-spermatocytes arrest during stage IV of the epithelial cycle with subsequent TUNEL-positive apoptosis resulting from improper synapsis, defects in the repair of DNA double-stranded breaks and synaptonemal complex formation. Moreover, we show nuclear accumulation of cyclin B1 with a subsequent premature increase in G 2 /M kinase activity in Nipa -/-spermatocytes. Together, these results reveal a novel role for NIPA in meiosis.

show abstract

“…Serum testosterone levels were determined using the Testosterone ELISA Kit from IBL-Hamburg. Flow cytometric analyses of testis cell types were carried out according to Jeyaraj et al (2002) using a BC FACSCalibur Flow Cytometer (BD Biosciences Clontech).…”

Section: Other Protocolsmentioning

confidence: 99%

DAZAP1, an hnRNP protein, is required for normal growth and spermatogenesis in mice

Hsu¹,

Chen²,

Lin³

et al. 2008

RNA

View full text Add to dashboard Cite

DAZAP1 (Deleted in Azoospermia Associated Protein 1) is a ubiquitous hnRNP protein that is expressed most abundantly in the testis. Its ability to shuttle between the nucleus and the cytoplasm and its exclusion from the transcriptionally inactive XY body in pachytene spermatocytes implicate it in mRNA transcription and transport. We generated Dazap1 mutant alleles to study the role of DAZAP1 in mouse development. Most mice homozygous for the null allele as well as a hypomorphic Fn allele died soon after birth. The few Dazap1Fn/Fn mice that survived could nonetheless live for more than a year. They appeared and behaved normally but were much smaller in size compared to their wild-type and heterozygous littermates. Both male and female Dazap1Fn/Fn mice were sterile. Males had small testes, and the seminiferous tubules were atrophic with increased numbers of apoptotic cells. The tubules contained many germ cells, including pachytene spermatocytes with visible XY-bodies and diplotene spermatocytes, but no post-meiotic cells. FACS analyses confirmed the absence of haploid germ cells, indicating spermatogenesis arrested right before the meiotic division. Female Dazap1Fn/Fn mice had small ovaries that contained normalappearing follicles, yet their pregnancy produced no progeny due to failure in embryonic development. The phenotypes of Dazap1 mutant mice indicate that DAZAP1 is not only essential for spermatogenesis, but also required for the normal growth and development of mice.

show abstract

Dynamics of Testicular Germ Cell Proliferation in Normal Mice and Transgenic Mice Overexpressing Rat Androgen-Binding Protein: A Flow Cytometric Evaluation1

Cited by 45 publications

References 43 publications

Differential hormonal regulation of estrogen receptors ERα and ERβ and androgen receptor expression in rat efferent ductules

Differential hormonal regulation of estrogen receptors ERα and ERβ and androgen receptor expression in rat efferent ductules

Targeted inactivation of nuclear interaction partner of ALK disrupts meiotic prophase

DAZAP1, an hnRNP protein, is required for normal growth and spermatogenesis in mice

Contact Info

Product

Resources

About