Dynamics of telopodes (telocyte prolongations) in cell culture depends on extracellular matrix protein

Niculite, Cristina Mariana; Regalia, T.; Gherghiceanu, Mihaela; Huică, Radu‐Ionuț; Surcel, Mihaela; Ursaciuc, Cornel; Leabu, Mircea; Popescu, L. M.

doi:10.1007/s11010-014-2215-z

Cited by 41 publications

(31 citation statements)

References 33 publications

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“…High levels of fibronectin determine the differentiation of cells and the loss of the self‐renewal markers Nanog and Oct4 . Furthermore, TCs were previously shown to adhere and spread better than fibroblasts in vitro . Taken together, these findings suggest that TCs synergistically act with stem cells to affect heart regeneration.…”

Section: Discussionmentioning

confidence: 78%

The secretome of myocardial telocytes modulates the activity of cardiac stem cells

Albulescu

Tănase

Codrici

et al. 2015

J Cellular Molecular Medi

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Telocytes (TCs) are interstitial cells that are present in numerous organs, including the heart interstitial space and cardiac stem cell niche. TCs are completely different from fibroblasts. TCs release extracellular vesicles that may interact with cardiac stem cells (CSCs) via paracrine effects. Data on the secretory profile of TCs and the bidirectional shuttle vesicular signalling mechanism between TCs and CSCs are scarce. We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate. Therefore, we studied the protein secretory profile using supernatants from mouse cultured cardiac TCs. We also performed a comparative secretome analysis using supernatants from rat cultured cardiac TCs, a pure CSC line and TCs-CSCs in co-culture using (i) high-sensitivity on-chip electrophoresis, (ii) surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and (iii) multiplex analysis by Luminex-xMAP. We identified several highly expressed molecules in the mouse cardiac TC secretory profile: interleukin (IL)-6, VEGF, macrophage inflammatory protein 1α (MIP-1α), MIP-2 and MCP-1, which are also present in the proteome of rat cardiac TCs. In addition, rat cardiac TCs secrete a slightly greater number of cytokines, IL-2, IL-10, IL-13 and some chemokines like, GRO-KC. We found that VEGF, IL-6 and some chemokines (all stimulated by IL-6 signalling) are secreted by cardiac TCs and overexpressed in co-cultures with CSCs. The expression levels of MIP-2 and MIP-1α increased twofold and fourfold, respectively, when TCs were co-cultured with CSCs, while the expression of IL-2 did not significantly differ between TCs and CSCs in mono culture and significantly decreased (twofold) in the co-culture system. These data suggest that the TC secretome plays a modulatory role in stem cell proliferation and differentiation.

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Section: Discussionmentioning

confidence: 78%

The secretome of myocardial telocytes modulates the activity of cardiac stem cells

Albulescu

Tănase

Codrici

et al. 2015

J Cellular Molecular Medi

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“…Smooth muscle proteins were identified in telocytes such as desmin, vimentin, α-SMA, smooth muscle myosin heavy chain, smoothelin, myosin light chain kinase, calponin, vinculin, and α-actinin. Telopode dynamics were studied in telocytes of the mouse heart [Suciuet al, 2010;Niculite et al, 2015]. Although the contractile elements and associated proteins were located in telocytes, they were not contractile, and their movement was restricted.…”

Section: Discussionmentioning

confidence: 99%

New Description of Telocyte Sheaths in the Bovine Uterine Tube: An Immunohistochemical and Scanning Microscopic Study

Abd‐Elhafeez

Soliman

2016

Cells Tissues Organs

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Telocytes are a special type of interstitial cells characterized by distinctive cellular extensions with alternating thin segments (podomers) and dilations (podoms). Telocytes establish contact with various cells and structures, but their role in the regulation of the function of many cell types is still obscure. The aim of the current study was to investigate the morphology, histochemistry, and immunohistochemistry of telocytes, and their distribution, organization, and morphometric measurements in different layers of the adult bovine uterine tube. Telocytes showed positive immunostaining for CD117, S-100 protein, vimentin, desmin, α-smooth muscle actin, tubulin, laminin, estrogen receptor-α, and progesterone receptor. They were organized in different types of sheaths: subepithelial, inner/outer perimuscular, and intramuscular sheaths. Telocytes were scattered in the lamina propria, in the muscular layer, and the serosa. According to their size, they were grouped into different types of telocytes: small, large, and giant telocytes. Small telocytes were the most common type and located in all layers; large telocytes were observed in the epithelium, lamina propria, and inner/outer perimuscular and intramuscular sheaths, and giant telocytes were found in the external layer of the outer perimuscular sheath. Telocytes were connected by thin and thick telopodes (fenestrated membranes). Fenestrated membranes enabled connections between telocytes along the entire muscular wall of the uterine tube. Telocytes established an extensive biological network of different types of cells and structures, including epithelial, muscular, and mast cells, blood vessels, glomus, and nerve fibers. We hypothesize that telocytes help to organize the functional coordination between different types of cells in the uterine tube.

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“…A growing number of studies have described telocytes in different mammalian tissues and organs . Concerning SGs, the only study published to date was performed on rat and human normal parotid glands and revealed that telocytes are present not only in periacinar areas similarly to exocrine pancreas, but also around ductal structures as also observed in bile ducts .…”

Section: Discussionmentioning

confidence: 99%

Telocytes in minor salivary glands of primary Sjögren's syndrome: association with the extent of inflammation and ectopic lymphoid neogenesis

Alunno

Ibba‐Manneschi

Bistoni

et al. 2015

J Cellular Molecular Medi

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It has been recently reported that telocytes, a stromal (interstitial) cell subset involved in the control of local tissue homeostasis, are hampered in the target organs of inflammatory/autoimmune disorders. Since no data concerning telocytes in minor salivary glands (MSGs) are currently available, aim of the study was to evaluate telocyte distribution in MSGs with normal architecture, non-specific chronic sialadenitis (NSCS) and primary Sjögren’s syndrome (pSS)-focal lymphocytic sialadenitis. Twelve patients with pSS and 16 sicca non-pSS subjects were enrolled in the study. MSGs were evaluated by haematoxylin and eosin staining and immunofluorescence for CD3/CD20 and CD21 to assess focus score, Tarpley biopsy score, T/B cell segregation and germinal center (GC)-like structures. Telocytes were identified by immunoperoxidase-based immunohistochemistry for CD34 and CD34/platelet-derived growth factor receptor α double immunofluorescence. Telocytes were numerous in the stromal compartment of normal MSGs, where their long cytoplasmic processes surrounded vessels and encircled both the excretory ducts and the secretory units. In NSCS, despite the presence of a certain degree of inflammation, telocytes were normally represented. Conversely, telocytes were markedly reduced in MSGs from pSS patients compared to normal and NSCS MSGs. Such a decrease was associated with both worsening of glandular inflammation and progression of ectopic lymphoid neogenesis, periductal telocytes being reduced in the presence of smaller inflammatory foci and completely absent in the presence of GC-like structures. Our findings suggest that a loss of MSG telocytes might have important pathophysiological implications in pSS. The specific pro-inflammatory cytokine milieu of pSS MSGs might be one of the causes of telocyte loss.

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Dynamics of telopodes (telocyte prolongations) in cell culture depends on extracellular matrix protein

Cited by 41 publications

References 33 publications

The secretome of myocardial telocytes modulates the activity of cardiac stem cells

The secretome of myocardial telocytes modulates the activity of cardiac stem cells

New Description of Telocyte Sheaths in the Bovine Uterine Tube: An Immunohistochemical and Scanning Microscopic Study

Telocytes in minor salivary glands of primary Sjögren's syndrome: association with the extent of inflammation and ectopic lymphoid neogenesis

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