Dynamics of Spirochetemia and Early PCR Detection of <i>Borrelia miyamotoi</i>

Karan, Ludmila S.; Makenov, Marat T.; Kolyasnikova, Nadezhda M.; Стуколова, О. А.; Топоркова, М. Г.; Olenkova, Olga

doi:10.3201/eid2405.170829

Cited by 42 publications

(45 citation statements)

References 29 publications

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“…syndrome. It complements the Russian study by Karan et al published in 2018 (28), which was conducted at the early stage on patients presenting with acute symptoms after a tick bite.…”

Section: Discussionsupporting

confidence: 58%

“…Erythema migrans was observed in 16.1% of the patients, but data are insufficient to rule out a previous infection with B. burgdorferi s.l. However the responsibility of B. miyamotoi in some cases of erythema migrans is probable since, in the study looking at the early stage of the tick-borne infection, 3% of patients with an erythema migrans had a positive blood PCR for B. miyamotoi (28). Our data suggest that the disease may be persistent, even on the long term and that this species of Borrelia may not cross-react with B. burgdorferi serology.…”

Section: Discussionmentioning

confidence: 66%

“…is the reappearanceor persistence of signs and symptoms after a classical treatment (26,27). Recently, a study conducted in Russia, confirmed the presence of B. miyamotoi in 70 of 473 patients at the early stage of signs and symptoms occurring after a tick bite (28). This study showed that the median time for detection of B. miyamotoi in blood was 4 days after inoculation.…”

Section: Introductionmentioning

confidence: 56%

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Borrelia miyamotoi: 43 Cases Diagnosed in France by Real-Time PCR in Patients With Persistent Polymorphic Signs and Symptoms

Franck¹,

Ghozzi²,

Pajaud³

et al. 2020

Front. Med.

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Background: Borrelia species are divided into three groups depending on the induced disease and the tick vector. Borrelia miyamotoi is a relapsing fever Borrelia but can induce symptoms related to Lyme disease. Discovered in 1995, it is found in ticks around the world. In France, this species of Borrelia has been isolated in ticks and rodents, but was not yet observed in humans. Objective: The aim of the study was to look for B. miyamotoi in symptomatic patients. Methods: Real-time PCR was performed on 824 blood samples from patients presenting symptoms of persistent polymorphic syndrome possibly due to tick bite, a syndrome recognized by the French Authority for Health, which is close to the post-treatment Lyme disease syndrome. PCR was also performed on 24 healthy control persons. The primers were specifically designed for this particular species of Borrelia. The sequence of interest of 94 bp is located on the glpQ gene. Sequencing of amplification products, randomly chosen, confirmed the amplification specificity. To better investigate cases, a clinical questionnaire was sent to the patients PCR-positive for B. miyamotoi and to their physician. Results: This search revealed a positive PCR for B. miyamotoi in the blood from 43 patients out of 824 (5.22%). PCR was negative in all control persons. A clinical chart was obtained from 31 of the 43 patients. A history of erythema migrans was reported in five of these 31 patients (16%). All patients complained about fatigue, joint pain and neuro-cognitive disorders. Some patients complained about respiratory problems (chest tightness and/or lack of air in 41.9%). Episodes of relapsing fever were reported by 11 of the 31 patients (35.5%). Chilliness, hot flushes and/or sweats were reported by around half of the patients. B. miyamotoi may not cross-react with B. burgdorferi serology. Conclusion: This study is the first to detect B. miyamotoi in human blood in France. This series of human B. miyamotoi infection is the largest in patients with long term persistent syndrome. Our data suggest that this infection may be persistent, even on the long term.

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“…syndrome. It complements the Russian study by Karan et al published in 2018 (28), which was conducted at the early stage on patients presenting with acute symptoms after a tick bite.…”

Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 56%

See 1 more Smart Citation

Borrelia miyamotoi: 43 Cases Diagnosed in France by Real-Time PCR in Patients With Persistent Polymorphic Signs and Symptoms

Franck¹,

Ghozzi²,

Pajaud³

et al. 2020

Front. Med.

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“…in human specimens, thus challenging prevailing thought regarding geographic distribution and prevalence of RFB infection [15]. For example, a recent study reported that approximately 23% of a cohort of 301 patients in Russia with confirmed Borrelia infection demonstrated positive molecular testing for B. miyamotoi [31]. RFB is also a growing concern in the Western USA, Central America, and South America [13,32].…”

Section: Discussionmentioning

confidence: 99%

Lyme Disease: Diversity of Borrelia Species in California and Mexico Detected Using a Novel Immunoblot Assay

Fesler

Shah²,

Middelveen

et al. 2020

Healthcare

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Background: With more than 300,000 new cases reported each year in the United States of America (USA), Lyme disease is a major public health concern. Borrelia burgdorferi sensu stricto (Bbss) is considered the primary agent of Lyme disease in North America. However, multiple genetically diverse Borrelia species encompassing the Borrelia burgdorferi sensu lato (Bbsl) complex and the Relapsing Fever Borrelia (RFB) group are capable of causing tickborne disease. We report preliminary results of a serological survey of previously undetected species of Bbsl and RFB in California and Mexico using a novel immunoblot technique. Methods: Serum samples were tested for seroreactivity to specific species of Bbsl and RFB using an immunoblot method based on recombinant Borrelia membrane proteins, as previously described. A sample was recorded as seropositive if it showed immunoglobulin M (IgM) and/or IgG reactivity with at least two proteins from a specific Borrelia species. Results: The patient cohort consisted of 90 patients residing in California or Mexico who met the clinical case definition of chronic Lyme disease. Immunoblot testing revealed that 42 patients were seropositive for Bbsl (Group 1), while 56 patients were seropositive for RFB (Group 2). Eight patients were seropositive for both Bbsl and RFB species. Group 1 included patients who were seropositive for Bbss (14), B. californiensis (eight), B. spielmanii (10), B. afzelii/B. garinii (10), and mixed infections that included B. mayonii (three). Group 2 included patients who were seropositive for B. hermsii (nine), B. miyamotoi (seven), B. turicatae (nine), and B. turcica (two). In the remaining Group 1 and Group 2 patients, the exact Borrelia species could not be identified using the immunoblot technique. Conclusions: Lyme disease is associated with a diverse group of Borrelia species in California and Mexico. Current testing for Lyme disease focuses on detection of Bbss, possibly resulting in missed diagnoses and failure to administer appropriate antibiotic therapy in a timely manner. The genetic diversity of Borrelia spirochetes must be considered in future Lyme disease test development.

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“…This test will be used in clinical practice in the future and it will improve serologic examination in clinical studies [10]. Therefore, molecular tests (PCR or real time PCR) are more appropriate and reliable methods for routine diagnosis at this moment [2,11].…”

Section: Introductionmentioning

confidence: 99%

Borrelia miyamotoi DNA in a patient suspected of Lyme borreliosis

Fiecek

Lewandowska

Roguska

et al. 2019

Preprint

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Background Clinical manifestations in infection caused by B. miyamotoi can mimick highly variable symptoms of Lyme disease. The aim of our studies was to detect DNA of B. miyamotoi spirochetes in clinical materials from patients suspected of neuroborreliosis(retrospectively).Methods Samples of blood serum and cerebrospinal fluid were collected from 133 patients with clinical manifestations of neuroborreliosis. Diagnosis was established by detection of IgM and / or IgG specific antibodies to B. burgdorferi with ELISA in both sera and CSF. Specificity of positive ELISA results in sera were confirmed with Western-blot test. Bacterial DNA from the collected material was extracted, amplified and sequenced.Results Among 133 patients with clinical manifestations of neuroborreliosis recognized in the years 2010-2018., DNA of B. miyamotoi was detected in CSF from 1 (0.8%) patient with extraocular optic neuritis of the left eye (GenBank accession No. MK674170 and MK674171).Conclusion Detection of B. miyamotoi in patients with central nervous system infections, will allow a better understanding of the epidemiology of infections caused by Borrelia sp. spirochetes. Patients with neurological symptoms and questionable serological findings are a serious diagnostic problem, due to failure to meet the criteria for neuroboreliosis. This indicates the need for further studies of patients with signs of CNS infection.

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Dynamics of Spirochetemia and Early PCR Detection of Borrelia miyamotoi

Cited by 42 publications

References 29 publications

Borrelia miyamotoi: 43 Cases Diagnosed in France by Real-Time PCR in Patients With Persistent Polymorphic Signs and Symptoms

Borrelia miyamotoi: 43 Cases Diagnosed in France by Real-Time PCR in Patients With Persistent Polymorphic Signs and Symptoms

Lyme Disease: Diversity of Borrelia Species in California and Mexico Detected Using a Novel Immunoblot Assay

Borrelia miyamotoi DNA in a patient suspected of Lyme borreliosis

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