Dynamics of spike time encoding in the olfactory bulb

Abstract

In the mammalian olfactory bulb (OB), gamma oscillations in the local field potential are generated endogenously during odor sampling. Such oscillations arise from dynamical systems that generate organized periodic behavior in neural circuits, and correspond to spike timing constraints at fine timescales. While the cellular and network mechanisms of gamma oscillogenesis in the OB are reasonably well established, it remains unclear how these fine-timescale dynamics serve to represent odors. Are patterns of spike synchrony on the gamma timescale replicable and odor-specific? Does the transformation to a spike-timing metric embed additional computations? To address these questions, we used OB slices to examine the spike timing dynamics evoked by "fictive odorants" generated via spatiotemporally patterned optogenetic stimulation of olfactory sensory neuron axonal arbors. We found that a small proportion of mitral/tufted cells phase-lock strongly to the fast oscillations evoked by fictive odorants, and exhibit tightly coupled spike-spike synchrony on the gamma timescale during this stimulation. Moreover, the specific population of synchronized neurons differed based on the "quality", but not the "concentration", of the fictive odorant presented, and was conserved across multiple presentations of the same fictive odorant. Given the established selectivity of piriform cortical pyramidal neurons for inputs synchronized on this timescale, we conclude that spike synchronization on a milliseconds timescale is a metric by which the OB encodes and exports afferent odor information in a concentration-invariant manner. As a corollary, mitral/tufted cell spikes that are not organized in time may not contribute effectively to the ensemble odor representation.

Coherent olfactory bulb gamma oscillations arise from coupling independent columnar oscillators

“…The action potentials of a subset of active neurons – presumed to be principal neurons owing to their localization to the mitral cell layer and deep EPL – were constrained with respect to the phase of evoked gamma oscillations ( Fig. 3 A ), consistent with previous findings ( 4 , 28 , 29 , 44 ). This phase constraint occurred with all methods of activation, though it was clearest in response to ChR2 stimulation ( Fig.…”