Dynamics of Sonoporation Correlated with Acoustic Cavitation Activities

Zhou, Yun; Cui, Jianmin; Deng, Cheri X.

doi:10.1529/biophysj.107.125617

Cited by 64 publications

(51 citation statements)

References 11 publications

(16 reference statements)

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“…Fast resealing of cell membrane porations are reported to occur in the order of milliseconds to seconds [55,62,63] after switching off the ultrasound. The fact that cell membrane permeabilization is rapidly decaying indicates that pores exist as longs as the oscillating microbubbles are present [31].…”

Section: Spatiotemporal Aspects Of Stable Cavitationmentioning

confidence: 99%

See 1 more Smart Citation

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Lentacker

Cock

Deckers

et al. 2014

Advanced Drug Delivery Reviews

656

592

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In the past two decades, research has underlined the potential of ultrasound and microbubbles to enhance drug delivery. However, there is less consensus on the biophysical and biological mechanisms leading to this enhanced delivery. Sonoporation, i.e. the formation of temporary pores in the cell membrane, as well as enhanced endocytosis is reported. Because of the variety of ultrasound settings used -and corresponding microbubble behavior, a clear overview is missing. Therefore, in this review, the mechanisms contributing to sonoporation are categorized according to three ultrasound settings: i) low intensity ultrasound leading to stable cavitation of microbubbles, ii) high intensity ultrasound leading to inertial cavitation with microbubble collapse, and iii) ultrasound application in the absence of microbubbles. Using low intensity ultrasound, the endocytotic uptake of several drugs could be stimulated, while short but intense ultrasound pulses can be applied to induce pore formation and the direct cytoplasmic uptake of drugs. Ultrasound intensities may be adapted to create pore sizes correlating with drug size. Small molecules are able to diffuse passively through small pores created by low intensity ultrasound treatment. However, delivery of larger drugs such as nanoparticles and gene complexes, will require higher ultrasound intensities in order to allow direct cytoplasmic entry.2

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Section: Spatiotemporal Aspects Of Stable Cavitationmentioning

confidence: 99%

“…The fast resealing of the cell membrane poration was demonstrated by rapid decline of intracellular calcium levels [50,57] and restoration of cell membrane potential [55,[62][63][64].…”

Section: Spatiotemporal Aspects Of Stable Cavitationmentioning

confidence: 99%

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Lentacker

Cock

Deckers

et al. 2014

Advanced Drug Delivery Reviews

656

592

View full text Add to dashboard Cite

show abstract

“…Acoustic cavitation has been identified as an important mechanism in many therapeutic ultrasound applications, including drug and gene release and delivery, [1][2][3][4][5] sonothrombolysis, 6-10 sonophoresis, [11][12][13] hemostasis, 14 and thermal ablation. 15,16 Current research focuses on increasing efficacy and illuminating the specific mechanisms by which acoustic cavitation induces a bioeffect.…”

Section: Introductionmentioning

confidence: 99%

Passive imaging with pulsed ultrasound insonations

Haworth¹,

Mast²,

Radhakrishnan³

et al. 2012

The Journal of the Acoustical Society of America

114

138

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Previously, passive cavitation imaging has been described in the context of continuous-wave high-intensity focused ultrasound thermal ablation. However, the technique has potential use as a feedback mechanism for pulsed-wave therapies, such as ultrasound-mediated drug delivery. In this paper, results of experiments and simulations are reported to demonstrate the feasibility of passive cavitation imaging using pulsed ultrasound insonations and how the images depend on pulsed ultrasound parameters. The passive cavitation images were formed from channel data that was beamformed in the frequency domain. Experiments were performed in an in vitro flow phantom with an experimental echo contrast agent, echogenic liposomes, as cavitation nuclei. It was found that the pulse duration and envelope have minimal impact on the image resolution achieved. The passive cavitation image amplitude scales linearly with the cavitation emission energy. Cavitation images for both stable and inertial cavitation can be obtained from the same received data set.

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“…We have previously employed a voltage clamp technique to monitor sonoporation (23)(24)(25)(26) in real time by measuring the transmembrane current (TMC) of single Xenopus oocytes. The ultrasound-induced localized membrane disruption allowed ions to flow nonselectively through the membrane, resulting in an increase of the TMC.…”

mentioning

confidence: 99%

Spatiotemporally controlled single cell sonoporation

Fan

Liu

Mayer

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

210

215

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This paper presents unique approaches to enable control and quantification of ultrasound-mediated cell membrane disruption, or sonoporation, at the single-cell level. Ultrasound excitation of microbubbles that were targeted to the plasma membrane of HEK-293 cells generated spatially and temporally controlled membrane disruption with high repeatability. Using whole-cell patch clamp recording combined with fluorescence microscopy, we obtained time-resolved measurements of single-cell sonoporation and quantified the size and resealing rate of pores. We measured the intracellular diffusion coefficient of cytoplasmic RNA/DNA from sonoporation-induced transport of an intercalating fluorescent dye into and within single cells. We achieved spatiotemporally controlled delivery with subcellular precision and calcium signaling in targeted cells by selective excitation of microbubbles. Finally, we utilized sonoporation to deliver calcein, a membrane-impermeant substrate of multidrug resistance protein-1 (MRP1), into HEK-MRP1 cells, which overexpress MRP1, and monitored the calcein efflux by MRP1. This approach made it possible to measure the efflux rate in individual cells and to compare it directly to the efflux rate in parental control cells that do not express MRP1.D espite the development of various approaches for transporting membrane-impermeant compounds (such as fluorescent markers, DNA, RNA, siRNA, proteins, peptides, and amino acids) into living cells (1-3), efficient intracellular delivery of bioactive agents for biomedical applications with minimal adverse effects remains challenging. In addition, it is desirable yet difficult to achieve local perturbation of intracellular processes, which requires subcellular molecular localization inside the living cell (4, 5).Sonoporation uses ultrasound to induce transient disruption of cell membranes (6-8), thereby enabling transport of membraneimpermeant compounds into the cytoplasm of living cells (6,(9)(10)(11). Without the need to use viral vectors, sonoporation enables the delivery of a wide range of bioactive agents with minimal inflammatory and immunological responses for both in vitro studies and in vivo applications (12-16). In addition, ultrasound application can be targeted to a specific volume of tissue in vivo noninvasively. These unique characteristics make sonoporation a compelling and versatile technology for nonviral drug and gene delivery.Sonoporation is typically performed for bulk treatment of a tissue volume in vivo or a large number of cells in vitro, often facilitated by microbubbles that are either injected in the vasculature or mixed in solution with suspended or attached cells. Ultrasound application induces cavitation of the microbubbles (17), signified by rapid volume expansion/contraction and/or collapse (18). These effects generate localized fluid flow, shear stress, and other mechanical or physical impact capable of affecting cells and structures nearby (7,19,20).However, the detailed processes supporting sonoporationmediated transmembrane and ...

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Dynamics of Sonoporation Correlated with Acoustic Cavitation Activities

Cited by 64 publications

References 11 publications

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Passive imaging with pulsed ultrasound insonations

Spatiotemporally controlled single cell sonoporation

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