Dynamics of soilborne <i>Rhizoctonia solani</i> in the presence of <i>Trichoderma harzianum</i>: effects on stem canker, black scurf and progeny tubers of potato

Wilson, Paula; Ketola, E. O.; Ahvenniemi, Paavo; Lehtonen, Mari; Valkonen, Jari P. T.

doi:10.1111/j.1365-3059.2007.01706.x

Cited by 44 publications

(33 citation statements)

References 29 publications

(47 reference statements)

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“…Decrease in the severity of symptoms was observed in the early stages of infection (i.e. during the first 7 days post-inoculation) and attributed to a transient antagonistic effect of T. harzianum (Wilson et al 2008). However, no studies have investigated the early (i.e.…”

Section: Discussionmentioning

confidence: 96%

“…Recently, Wilson et al (2008) investigated the dynamics of R. solani on potato plants in the presence of T. harzianum. These authors noted a decrease in the severity of symptoms caused by R. solani during the first 7 days post-inoculation and attributed this observation to a transient antagonistic effect of T. harzianum at the early stage of the interaction.…”

Section: Introductionmentioning

confidence: 99%

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Trichoderma harzianum elicits defence response genes in roots of potato plantlets challenged by Rhizoctonia solani

2008

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Biological control of Rhizoctonia solani with Trichoderma harzianum has been demonstrated in several studies. However, none have reported the dynamics of expression of defence response genes. Here we investigated the expression of these genes in potato roots challenged by R. solani in the presence/ absence of T. harzianum Rifai MUCL 29707. Analysis of gene expression revealed an induction of PR1 at 168 h post-inoculation (hpi) and PAL at 96 hpi in the plants inoculated with T. harzianum Rifai MUCL 29707, an induction of PR1, PR2 and PAL at 48 hpi in the plants inoculated with R. solani and an induction of Lox at 24 hpi and PR1, PR2, PAL and GST1 at 72 hpi in the plants inoculated with both organisms. These results suggest that in the presence of T. harzianum Rifai MUCL 29707, the expression of Lox and GST1 genes are primed in potato plantlets infected with R. solani at an early stage of infection.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Trichoderma harzianum elicits defence response genes in roots of potato plantlets challenged by Rhizoctonia solani

2008

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“…T. virens and T. harzianum have been shown to be effective at controlling stem canker and black scurf, as well as increasing tuber yield (Tsror et al, 2001;Brewer & Larkin, 2005and Wilson et al, 2008a, 2008b. Van den Boogert & Luttikholt (2004) by using Verticillium biguttatum, Wilson et al (2008a) by applying T. harzianum and Lahlali & Hijri (2010) by testing T. artroviride have been reported the efficiency of these antagonistic fungal biota agents against AG3-PT in potato.…”

Section: Harzianum T Reesei T Virens and T Viridementioning

confidence: 99%

“…Trichoderma virens and T. harzianum have been shown to be effective at controlling stem canker and black scurf, as well as increasing tuber yield (Tsror et al, 2001;Brewer & Larkin, 2005;Wilson et al, 2008a and2008b).). Kurzawińska (2006) also demonstrated an antagonistic effect of several Trichoderma species on Helminthosporium solani, providing evidence for the potential suppression of silver scurf by Trichoderma.…”

Section: Introductionmentioning

confidence: 99%

In vitro Antagonistic Activity of Trichoderma harzianum against Rhizoctonia solani The Causative Agent of Potato Black Scurf and Stem Canker

Ibrahim

2017

Egypt. J. Bot.

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IN EGYPT Potato (Solanum tuberosum L.) is considered one of the most important vegetable crops as well as many others countries in the world. It plays an important role in the Egyptian agricultural economy, not for local consumption but also for exportation especially to Europe, Russia and Arabic countries. However, potato crop suffer from more than 40 pests and diseases caused by insects, nematodes, viruses, bacteria and fungi, of which black scurf and stem canker induced by Rhizoctonia solani is probably the most serious disease. The world yield losses caused by R. solani were estimated to 5-15%. R. solani is an unspecialized parasite, survive in soil in the absence of host plant and make itself a very difficult pathogen to manage. Reduction or elimination of soil borne inoculum is the only effective solution to overcome the problem and this may be achieved through the application of various control measures of which fungal antagonists consider among the most important tactic. Although R. solaniis usually controlled through the application of chemicals, the serious ecological and financial toll of this fungus has prompted for research on biopesticides as a viable alternative. Trichoderma spp. were well-known fungi often used for the biological control of crop pests, whose anti-fungal mechanisms include competition for the substrate, antibiosis and/or mycoparasitism. Five isolates of Trichoderma harzianum were tested in vitro for their antagonistic potential against Rhizoctonia solani. Both Trichoderma harzianum and Rhizoctonia solani were identified by molecular and morphological methods. In dual culture of all isolates were found antagonistic to the growth of R. solani. The hyphal interaction studied using light microscopy revealed destructive mycoparasitism of R. solani by T. harzianum. The method of mycoparasitism was sparse to intense coiling of R. solani followed by disintegration, disorganization and death of R. solani mycelium.

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“…The main task would be to develop a biocontrol strategy. Biological control of R. solani by Trichoderma is well known (Harman 2006) and has been reported for infections of potato by R. solani (Brewer and Larkin 2005;Wilson et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Construction of new GFP-tagged fusants for Trichoderma harzianum with enhanced biocontrol activity

Kowsari¹,

Motallebi²,

Zamani³

2014

Journal of Plant Protection Research

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Abstract:Trichoderma is one of the most exploited biocontrol agents for the management of plant diseases. In biocontrol ecology, the critical factors are detection, and the monitoring and recovery of specific biocontrol agents either naturally present or deliberately released into the environment. Protoplast fusion is an appropriate tool for the improvement of biocontrol Trichoderma strains. Protoplast isolation from Trichoderma harzianum was achieved using 24 h culture age, 6.6 mg/ml Novazym L 1412 at 30°C which resulted the maximum protoplast yield of 5 × 10 8 /ml. The self-fused protoplasts were regenerated and 12 fusants were selected based on their growth rate on 2% colloidal chitin medium. Next, a comparison was done for chitinase and antagonistic activity. Transcriptomic analysis based on quantitative real-time RT-PCR, demonstrated that T8-05 fusant expressed 1.5 fold of chit42 transcript as compared with the parental line. This fusant with 7.02±0.15U chitinase activity showed a higher growth inhibition rate (100%) than the parent strain, against Rhizoctonia solani. To obtain a genetically marked fusant that can be used as a biomonitor, the fusant was cotransformed with the gfp and amdS genes. The morphology and viability of selected cotransformant (FT8-7MK-05-2) was similar to the parent.Green fluorescing conidia were observed within the first 2 days of incubation in the soil, and this was followed by the formation of chlamydopores after 60 days. The colonisation of the gfp-tagged fusant was also monitored visually on R. solani sclerotia by scanning electron microscopy. Production of gfp-tagged fusant of Trichoderma spp. provides a potentially useful tool for monitoring hyphal growth patterns and the population of biocontrol agent isolates introduced into environmental systems.

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Dynamics of soilborne Rhizoctonia solani in the presence of Trichoderma harzianum: effects on stem canker, black scurf and progeny tubers of potato

Cited by 44 publications

References 29 publications

Trichoderma harzianum elicits defence response genes in roots of potato plantlets challenged by Rhizoctonia solani

Trichoderma harzianum elicits defence response genes in roots of potato plantlets challenged by Rhizoctonia solani

In vitro Antagonistic Activity of Trichoderma harzianum against Rhizoctonia solani The Causative Agent of Potato Black Scurf and Stem Canker

Construction of new GFP-tagged fusants for Trichoderma harzianum with enhanced biocontrol activity

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