2016
DOI: 10.1038/nature18647
|View full text |Cite
|
Sign up to set email alerts
|

Dynamics of ribosome scanning and recycling revealed by translation complex profiling

Abstract: Regulation of messenger RNA translation is central to eukaryotic gene expression control. Regulatory inputs are specified by them RNA untranslated regions (UTRs) and often target translation initiation. Initiation involves binding of the 40S ribosomal small subunit (SSU) and associated eukaryotic initiation factors (eIFs)near the mRNA 5′ cap; the SSU then scans in the 3′ direction until it detects the start codon and is joined by the 60S ribosomal large subunit (LSU) to form the 80S ribosome. Scanning and othe… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

37
273
2
1

Year Published

2017
2017
2023
2023

Publication Types

Select...
10

Relationship

0
10

Authors

Journals

citations
Cited by 198 publications
(313 citation statements)
references
References 44 publications
37
273
2
1
Order By: Relevance
“…Typhimurium samples (Additional file 1: Figure S8). These shorter reads were also consistent with recent reports of ribosomal subunits in a variety of distinct configurations, observed from translation complex profiling in the eukaryote Saccharomyces cerevisiae [42]. Whether similar patterns of read length distributions can be observed in eukaryotic ribo-seq datasets remains to be determined, although conceptually the method and metrics described herein are fully extendable to eukaryotic datasets.…”
Section: Discussionsupporting
confidence: 88%
“…Typhimurium samples (Additional file 1: Figure S8). These shorter reads were also consistent with recent reports of ribosomal subunits in a variety of distinct configurations, observed from translation complex profiling in the eukaryote Saccharomyces cerevisiae [42]. Whether similar patterns of read length distributions can be observed in eukaryotic ribo-seq datasets remains to be determined, although conceptually the method and metrics described herein are fully extendable to eukaryotic datasets.…”
Section: Discussionsupporting
confidence: 88%
“…Rather our hope lies in the systematic examination of the influence of pertinent experimental parameters exemplified by Heyer et al [21], and the power of approaches such as that developed by O’Connor et al [64] to infer the sequence biases of different protocols. Also, a diversity of protocols can yield exciting results, such as the combination of fixation and serial sucrose gradient ultracentrifugation that was recently used to sequence the footprints of scanning initiation complexes [67]. …”
Section: Discussionmentioning
confidence: 99%
“…However, ribosome profiling does not provide information about scanning ribosomes, since the interaction between the 40S ribosome and the mRNA is weak, and so any footprint would be susceptible to dissociation from the 40S [78]. Formaldehyde cross-linking of the scanning 40S and mRNA freezes this fragile complex, and subsequent 40S isolation allows profiling of the scanning pre-initiation complex – a methodology called translation complex profiling sequencing, or TCP-Seq [79] (Figure 4e). Three distinct populations of 40S footprints sizes (19, 29, 37 nt) reflect the sequential changes in conformation and/or assembly/dissociation of factors on the translation initiation complex.…”
Section: Profiling Of Scanning Ribosomementioning
confidence: 99%