Dynamics of progestogenic activity in serum following administration of Ligusticum chuanxiong

Lim, Lis Sa Elissa; Shen, Ping; Gong, Yiwei; Lee, Lawrence S.; Yong, Eu Leong

doi:10.1016/j.lfs.2006.03.039

Cited by 12 publications

(4 citation statements)

References 34 publications

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“…7). Similar result was reported by Lim et al (2006b) who found the serum progestogenic activity was significantly elevated after oral administration of LC extract in rats. This result indicates that exogenous progestogen can be monitored with the plasmid.…”

Section: Response To Animal Serumsupporting

confidence: 90%

Construct and validation of progesterone-responsive luciferase reporter plasmid to detect progestogenic and anti-progestogenic activity

Ahmed

Cheng

2013

Bangl. vet.

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Chemical and immunological analyses used to detect progesterone and its agonists and antagonists are unable to detect biological activities of unknown compounds. The objective of the experiment was to develop a progesterone-responsive reporter plasmid to detect progestogens and anti-progestogens. Progesterone-responsive luciferase reporter plasmid, pGL3-2PRE-TATA, was constructed by inserting two progesterone-response elements (PRE) and the TATA box at the multiple cloning site of pGL3 basic vector. T47D human breast cancer cells were co-transfected with c pGL3-2PRE-TATA and pCH110 plasmid. The transfected cells were exposed to progesterone, anti-progesterone, herb extracts or animal serum to induce the luciferase gene expression. Cells were lysed, and cell lysates were used to measure the luciferase and ?-galactosidase expression level as well as total protein content. Luciferase activity was normalized by ?-galactosidase activity and total protein content. The constructed plasmid was sensitive to progestogens and anti-progestogens in a dose-responsive manner. The pGL3-2PRE-TATA plasmid can be used to detect the progestogenic and anti-progestogenic activity of known and unknown compounds. DOI: http://dx.doi.org/10.3329/bvet.v30i1.16280 Bangl. vet. 2013. Vol. 30, No. 1, 10-19

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Section: Response To Animal Serumsupporting

confidence: 90%

Construct and validation of progesterone-responsive luciferase reporter plasmid to detect progestogenic and anti-progestogenic activity

Ahmed

Cheng

2013

Bangl. vet.

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“…Riligustilide, one of dimeric phthalides isolated from LC, displays a weak progesterone-like activity, whereas another dimeric phthalide compound, (3Z')-(3Z'R,6'R,3R,6R,7R)-3,8-dihydro-6.6' ,7.3a'-diligustilide, is a potent and specific activator of progesterone receptor. The in vivo progestogenic activity of LC extract in male Sprague-Dawley rats probably has utility for progesterone-replacement therapy (Lim et al, 2006a). These bioactive phthalides and their parent extracts may have utility for the treatment of conditions requiring progesterone action (Lim et al, 2006b).…”

Section: Progesterone-like Activitymentioning

confidence: 99%

Ligusticum chuanxiongHort: A review of chemistry and pharmacology

Ran¹,

Ma²,

Peng³

et al. 2011

Pharmaceutical Biology

165

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A large number of pharmacological and chemical studies during the last 10 years have demonstrated the vast medicinal potential of LC. It is still very clear that LC is a plant with widespread use now and also with extraordinary potential for the future. The documents strongly support the view that LC has beneficial therapeutic properties and indicates its potential as an effective adaptogenic herbal remedy.

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“…(Chuanxiong), is a well-known traditional Chinese medicine that is commonly prescribed for the treatment of migraine and various cardiovascular diseases, such as angina pectoris and ischemic stroke [1][2][3] and endocrine disorders [4] in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Metabolism of Chuanxiong Rhizoma decoction: Identification of the metabolites in WZS-miniature pig urine

et al. 2015

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Dynamics of progestogenic activity in serum following administration of Ligusticum chuanxiong

Cited by 12 publications

References 34 publications

Construct and validation of progesterone-responsive luciferase reporter plasmid to detect progestogenic and anti-progestogenic activity

Construct and validation of progesterone-responsive luciferase reporter plasmid to detect progestogenic and anti-progestogenic activity

Ligusticum chuanxiongHort: A review of chemistry and pharmacology

Metabolism of Chuanxiong Rhizoma decoction: Identification of the metabolites in WZS-miniature pig urine

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