Dynamics of phenolic compounds in leaves Fragaria ananassa Duch. after treatment with different forms of chitosan

Subin, O.V.; Likhanov, Artur; Sereda, O.; Klyuvadenko, Andrii A.; Melnychuk, M.

doi:10.5114/bta.2020.97881

Cited by 3 publications

(2 citation statements)

References 33 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flavonoids have been widely investigated for their free radical scavenging properties, which contribute to the prevention of cancer (Zhao et al, 2019 ; Berk et al, 2022 ) and cardiovascular diseases (Rees et al, 2018 ; Russo et al, 2019 ). Polyphenolic compounds, including flavonoids, can serve as supplements in the prevention and treatment of cardiovascular and inflammatory diseases (Subin et al, 2020 ; Chen and Zhang, 2021 ). Additionally, flavonoids exhibit immunostimulatory effects and can mitigate allergic responses (Hayat et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

Setiawati,

Arofah,

Nurzaman

et al. 2023

bta

View full text Add to dashboard Cite

Chrysanthemum ( Chrysanthemum morifolium ) contains secondary metabolites, such as flavonoid compounds, especially luteolin-7-glucoside and quercetin-3-O-rhamnoside (quercitrin), in its tissues. Utilizing sucrose as an elicitor through callus culture presents an alternative method to enhance the production of secondary metabolites. This research aimed to determine the best sucrose concentration and harvest time for maximizing quercitrin content in chrysanthemum callus culture. The research employed a completely randomized design with four treatment groups: 0, 30, 45, and 60 g/l of sucrose added to MS medium containing 4 ppm 2,4-dichlorophenoxyacetic acid (2,4-D). Callus samples were harvested on the 15th and 30th days of culture. The observed parameters included callus morphology (color and texture), fresh weight, dry weight, the diameter of the callus, and quercitrin content analyzed using high-performance liquid chromatography. The results showed that all callus cultures exhibited intermediate textures and varied colors, predominantly shades of brown. The treatment involving 45 g/l of sucrose with a 30th-day harvest yielded the highest fresh weight, dry weight, and quercitrin content, namely 2.108 g, 0.051 g, and 0.437 mg/g DW, respectively. Notably, the quercitrin content exhibited a 63.67% increase compared to the control.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

Setiawati,

Arofah,

Nurzaman

et al. 2023

bta

View full text Add to dashboard Cite

show abstract

“…The cytotype and ploidy are often overlooked in plant collection, which may explain the difference in quantitative levels of individual flavonoids and other metabolites in plant material [ 27 ]. In addition, plants are characterized by the daily dynamics of the content of phenolic compounds in the vegetative organs [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Flavonoids in Invasion Strategy of Solidago canadensis L.

Likhanov

Oliinyk²,

Pashkevych

et al. 2021

Plants

View full text Add to dashboard Cite

This study provides data on the problem of potential complexation of phenolic compounds synthesized by the plants Solidago canadensis L. and Solidago gigantea Ait. with ammonium forms of nitrogen, partly immobilized in the soil. A comparative analysis of secondary metabolites of the studied plants was performed by HPLC. The leaves of invasively active Solidago canadensis contain nine times more rutin than the plants of Solidago gigantea. Adding to the leaf extracts (v/v1/20) aqueous ammonia solution to pH 8.0 on the chromatograms decreases the intensity or completely causes peaks of flavonoids to disappear; instead, there are peaks of new polar substances (tR 1.5 and 2.0 min). The selective effect of the phenol-ammonium complex on various plant species was revealed. At a concentration of 20 μg/mL, these substances stimulated the formation of lateral roots in soybean seedlings and chrysanthemum cuttings. The suppression of root growth in radish seedlings occurred at a concentration of flavonoids in the extract of 25 μg/mL. In addition, a positive chemotaxis of the Pseudomonas putida (PGPR) was detected in the nitrogen-containing complex based on rutin (5 μg/mL). The identified feature allows PGPR colonization of the root system of Solidago canadensis with corresponding changes in the structure of the microbial community. The ability of the obtained nitrogen-containing polar complexes to regulate the growth processes of plants at extremely low concentration points to promising research in this direction.

show abstract

The Influence of Chitosanes on the Dynamics of the Content of Phenolic Compounds in Tomato Leaves

Bohoslavets

2023

Biolohichni systemy

View full text Add to dashboard Cite

The aim of this work was to study the influence of chitosans of different molecular weight on the dynamics of the content of phenolic compounds in tomato plants of the 'Zagadka' variety. In the experiment, tomato plants treated with chitosan with a molecular weight of 50-190 kDa and 310-375 kDa were studied. Using the method of high-performance thin-layer chromatography (HPTLC), biochemical profiling of the extracts of the studied plants was performed. Differences in the primary reactions of plants to chitosans of different molecular weights were revealed. Low molecular chitosan caused a significant increase in the content of phenols and, accordingly, increased the antioxidant potential of leaf tissues. The increase in the content of phenols in response to the action of low-molecular-weight chitosan occurs due to the activation of phenylpropanoid synthesis and an increase in the total antioxidant potential, which indicates the mobilization of the plant organism against a potential pathogen. Thus, an hour after the treatment of plants with a solution of low molecular weight chitosan, the content of rutin in the leaves was 3.36 mg/g, and after 18 hours - 5.56 mg/g. The content of chlorogenic and caffeic acids in the leaves increased in 12 hours. In 18 hours, the content of chlorogenic acid was 1.64 mg/g, and caffeic acid was 0.18 mg/g. This may be due to the gradual decomposition of chlorogenic acid into its constituent components under the influence of low molecular weight chitosan, which is accompanied by the release of caffeic acid. A positive correlation was established between the pool of chlorogenic and caffeic acid (r = 0.995; p < 0.06). Therefore, depending on the molecular weight, chitosan causes significant changes in the synthesis of phenylpropanoids and biochemical transformation of complex and simple esters of hydroxycinnamic acids in tomato plants. The high-molecular chitosan solution caused a decrease in the number of compounds with high antioxidant potential in tomato leaves, which indicates the activation of another plant defense system. The content of rutin in leaves treated with high-molecular-weight chitosan after 18 hours was 1.32 mg/g, which is four times less compared to the treatment of plants with low-molecular-weight chitosan. At the same time, in response to treatment with high molecular weight chitosan, the content of caffeic acid in the leaves increased significantly. The decrease in the content of phenols is associated with the isolation of tissues from a potential threat, which is aimed at its neutralization. The result of such a reaction is the oxidation of phenolic compounds, additional lignification and suberinization of cell walls. The activation of various induced immunity reactions in the plant organism already in the first hours after eating low-molecular or high-molecular chitosan indicates the existence of a system of differential recognition of complex bipolymers by plants.

show abstract

Dynamics of phenolic compounds in leaves Fragaria ananassa Duch. after treatment with different forms of chitosan

Cited by 3 publications

References 33 publications

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

The Role of Flavonoids in Invasion Strategy of Solidago canadensis L.

The Influence of Chitosanes on the Dynamics of the Content of Phenolic Compounds in Tomato Leaves

Contact Info

Product

Resources

About