Dynamics of membrane fatty acid composition of succinic acid-producing Anaerobiospirillum succiniciproducens

Hwang, Hee Jin; Choi, Se Yeon; Lee, Sang Yup; Choi, Joing-il; Han, Se Jong; Lee, Pyung Cheon

doi:10.1016/j.jbiotec.2014.11.028

Cited by 11 publications

(11 citation statements)

References 19 publications

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“…In this study, it was found that the fatty acid composition of cell membrane could be regulated for resisting acid stress. It was reported that higher proportion of unsaturated fatty acid was beneficial for some basic metabolisms (Hwang et al 2015). Also, the coenzyme Q shuttle in the phospholipid bilayers was accelerated to promote the proton motive force across membrane, which thus promoted the formation of ATP (Kanjee and Houry 2013).…”

Section: Discussionmentioning

confidence: 98%

System-level understanding of the potential acid-tolerance components of Acidithiobacillus thiooxidans ZJJN-3 under extreme acid stress

Feng

Yang

2015

Extremophiles

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In previous study, two extremely acidophilic strains Acidithiobacillus thiooxidans ZJJN-3 (collection site: bioleaching leachate) and ZJJN-5 (collection site: bioleaching wastewater) were isolated from a typical industrial bio-heap in China. Here, we unraveled the potential acid-tolerance components of ZJJN-3 by comparing the physiological differences with ZJJN-5 under different acid stresses. The parameters used for comparison included intracellular pH (pHin), capsule morphology, fatty acid composition of cell membrane, transcription of key molecular chaperones, H(+)-ATPase activities and NAD(+)/NADH ratio. It was indicated that the acid-tolerance of A. thiooxidans ZJJN-3 was systematically regulated. Capsule first thickened and then shed off along with increased acid stress. Cell membrane maintained the intracellular stability by up-regulating the proportion of unsaturated fatty acid and cyclopropane fatty acids. Meanwhile, the transcription of key repair molecular chaperones (GrpE-DnaK-DnaJ) was up-regulated by 2.2-3.5 folds for ensuring the proper folding of peptide. Moreover, low pHin promoted ZJJN-3 to biosynthesize more H(+)-ATPase for pumping H(+) out of cells. Furthermore, the NAD(+)/NADH ratio increased due to the decreased H(+) concentration. Based on the above physiological analysis, the potential acid-tolerance components of A. thiooxidans ZJJN-3 were first proposed and it would be useful for better understanding how these extremophiles responded to the high acid stress.

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Section: Discussionmentioning

confidence: 98%

System-level understanding of the potential acid-tolerance components of Acidithiobacillus thiooxidans ZJJN-3 under extreme acid stress

Feng

Yang

2015

Extremophiles

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“…Hagve et al reported that passive diffusion of lipophilic compounds was improved when the degree of unsaturation of some de ned phospholipid molecular species was increased [17]. In addition, elongating the saturated fatty acids or converting them to unsaturated fatty acids is a common bacterial adaptation strategy to resist acid stress [18]. Thus, we aim to enhance the transmembrane transfer of NR in A. succinogenes by breeding acid-resistant mutants.…”

Section: Discussionmentioning

confidence: 99%

Microbial fuel cell assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes

Zheng

et al. 2020

Preprint

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Background: The global production of glycerol has been increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succinate-producing candidate, cannot grow on glycerol anaerobically, as it needs a terminal electron acceptor to maintain the balance of intracellular NADH and NAD+.Results: Microbial fuel cells (MFC) assisted succinate production was evaluated using neutral red as an electron shuttle to recover the glycerol utilization. Firstly, an acid tolerant mutant JF1315 was selected by atmospheric and room temperature plasma (ARTP) mutagenesis aiming to improve transmembrane transfer of neutral red (NR). Additionally, MFC was established to increase the ratio of oxidized NR to reduced NR. By combining these two strategies, ability of JF1315 for glycerol utilization was significantly enhanced, and 23.92 g/L succinate was accumulated with a yield of 0.88 g/g from around 30 g/L initial glycerol. In addition, relative constant and high value above 300 mV was obtained for at least 48 h.Conclusions: A novel MFC-assisted system was established to improve glycerol utilization by A. succinogenes for succinate and electricity production, making this system as a platform for chemicals production and electrical supply simultaneously.

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“…Consistent with the optimization results from CCD, the rapid growth of PC-3 cells depended on high concentrations of EGF. FGF is a commonly used serum-free medium additive, which can stimulate DNA synthesis and cell proliferation in a variety of cells [ 26 ]. The unsaturated fatty acid linoleic acid as one of the commonly used in serum-free medium can promote cell proliferation and also promote cell expression of antibodies [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Vitamins C and E are antioxidants. Succinic acid provides the required lipid for cell membrane synthesis and the water-soluble lipids for cell growth [ 25 , 26 ]. Growth factors are supplemental factors which are necessary to maintain the survival, proliferation and differentiation of cells in vitro.…”

Section: Methodsmentioning

confidence: 99%

Use of real-time cellular analysis and Plackett-Burman design to develop the serum-free media for PC-3 prostate cancer cells

et al. 2017

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In this study, we developed a rapid strategy to screen a serum-free medium for culturing the anchorage-dependent PC-3 prostate cancer cells, which was going to be prepared in large scale to generate GM-CSF/TNFα-surface-modified whole cell prostate cancer vaccine. Automated real-time cellular analysis as a rapid and non-invasive technology was used to monitor the growth of PC-3 cells in 16-well plates. At the same time, Plackett-Burman design was employed to identify the most influential formulation by integrating relevant information statistically. The effects of the 16 selected factors were evaluated during exponential cell growth and three medium constituents (EGF, FGF and linoleic acid) were identified to have significant effects on the cell growth. Subsequently, the response surface methodology with central composite design was applied to determine the interactions among the three factors so that these factors were optimized to improve cell growth. Finally, the prediction of the best combination was made under the maximal response to optimize cell growth by Design-Expert software 7.0. A total of 20 experiments were conducted to construct a quadratic model and a second-order polynomial equation. With the optimized combination validated by the stability test of serial passaging PC-3 cells, the serum-free medium had similar cell density and cell viability to the original serum medium. In summary, this high-throughput scheme minimized the screening time and may thus provide a new platform to efficiently develop the serum-free media for adherent cells.

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Dynamics of membrane fatty acid composition of succinic acid-producing Anaerobiospirillum succiniciproducens

Cited by 11 publications

References 19 publications

System-level understanding of the potential acid-tolerance components of Acidithiobacillus thiooxidans ZJJN-3 under extreme acid stress

System-level understanding of the potential acid-tolerance components of Acidithiobacillus thiooxidans ZJJN-3 under extreme acid stress

Microbial fuel cell assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes

Use of real-time cellular analysis and Plackett-Burman design to develop the serum-free media for PC-3 prostate cancer cells

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