Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers

Baxter, Nielson T.; Schmidt, Alexander W.; Venkataraman, Arvind; Kim, Kwi S.; Waldron, Clive; Schmidt, Thomas M.

doi:10.1128/mbio.02566-18

Cited by 535 publications

(480 citation statements)

References 44 publications

Supporting

Mentioning

426

Contrasting

Unclassified

Order By: Relevance

“…Moreover, we showed that MMP could to a certain extent be predicted from stool microbiota community composition, to a greater extent than can raw stool SCFA content. These data are consistent with a recent study where participants with particular microbiota compositions were more likely to yield higher butyrate concentrations in the stool after consuming resistant starch (Baxter et al, 2019).…”

Section: Discussionsupporting

confidence: 92%

Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota

Gurry

Nguyen

et al. 2020

Preprint

View full text Add to dashboard Cite

The human gut microbiota is known for its highly heterogeneous composition across different individuals. However, relatively little is known about functional differences in its ability to ferment complex polysaccharides. Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs. We also show that aspects of these MMPs can be predicted from composition using 16S rRNA sequencing. From experiments performed using the same dietary fibers in vivo, we demonstrate that an ingested bolus of fiber is almost entirely consumed by the microbiota upon passage. We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production. Taken together, these data suggest that personalized dietary fiber supplementation based on an individual’s MMP is an attractive therapeutic strategy for treating diseases associated with SCFA production.

show abstract

Section: Discussionsupporting

confidence: 92%

Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota

Gurry

Nguyen

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Biosynthesis of vitamins by probiotic bacteria has been reviewed by (Gu & Li, ), showing that different probiotic species could be involved in different vitamin synthesis pathways. On the other hands, there is a hypothesis that short‐chain fatty acids derived from gut bacterial fermentation of dietary fibre may improve gut health by regulating host immune system (Baxter et al, ). Although many studies have found that probiotic diet improved growth and immune responses in different species, interaction among epithelium, gut bacteria and mucosal immune system in fish intestine remains uncertain.…”

Section: Discussionmentioning

confidence: 99%

Effects of two dietary probiotics ( Bacillus subtilis or licheniformis ) with two prebiotics (mannan or fructo oligosaccharide) in Japanese eel, Anguilla japonica

et al. 2019

View full text Add to dashboard Cite

A 12‐week feeding trial was conducted to investigate the effects of two dietary probiotics; Bacillus subtilis KCTC 2217 or Bacillus licheniformis KCCM 11775 with two prebiotics; mannan oligosaccharide (MOS) or fructooligosaccharide (FOS) in Japanese eel. Fish averaging 12.8 ± 0.47 g (mean ± SD) were randomly distributed into five treatments with triplicate tanks. A basal control diet (CON) and four synbiotic diets supplementing B. subtilis + MOS (BSM), B. subtilis + FOS (BSF), B. licheniformis + MOS (BLM), and B. licheniformis + FOS (BLF). Weight gain and specific growth rate of fish fed all synbiotic diets were higher than those of fish fed CON. Immune‐related gene expression of heat shock protein 70 and immunoglobulin M of fish fed BSF and BLM were significantly higher than those of fish fed CON. Fish fed BSF and BLM had significantly higher intestinal villi length than those of fish fed BLF and CON (p < .05). Disease resistance against Aeromonas hydrophila of fish fed all synbiotic diets were significantly higher than those of fish fed CON (p < .05). Therefore, these results indicated that dietary B. subtilis with FOS (BSF) and B. licheniformis with MOS (BLM) could have beneficial effects on intestinal morphology, and immune‐related gene expression in Japanese eel.

show abstract

“…We transferred the filtrate to 1.5 mL screw cap vials with 100 uL inserts for high performance liquid chromatography analysis (HPLC) and then randomized them. We quantified acetate, propionate, and butyrate concentrations using a refractive index detector as part of a Shimadzu HPLC system (Shimadzu Scientific Instruments, Columbia, MD) as previously described (8). Briefly, we used a 0.01 N H 2 SO 4 mobile phase through an Aminex HPX87H column (Bio-Rad Laboratories, Hercules, CA).…”

Section: Methodsmentioning

confidence: 99%

Dietary xanthan gum alters antibiotic efficacy against the murine gut microbiota and attenuatesClostridioides difficilecolonization

Schnizlein

Vendrov

Edwards

et al. 2019

Preprint

View full text Add to dashboard Cite

250 words): 28 29Dietary fiber provides a variety of microbiota-mediated benefits ranging from anti-30 inflammatory metabolites to pathogen colonization resistance. A healthy gut microbiota protects 31against Clostridioides difficile colonization. Manipulation of these microbes through diet may 32 increase colonization resistance to improve clinical outcomes. The primary objective of this 33 study was to identify how the dietary fiber xanthan gum affects the microbiota and C. difficile 34 colonization. 35We added 5% xanthan gum to the diet of C57Bl/6 mice and examined its effect on the 36 microbiota through 16S rRNA-gene amplicon sequencing and short-chain fatty acid analysis. 37

show abstract

Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers

Cited by 535 publications

References 44 publications

Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota

Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota

Effects of two dietary probiotics ( Bacillus subtilis or licheniformis ) with two prebiotics (mannan or fructo oligosaccharide) in Japanese eel, Anguilla japonica

Dietary xanthan gum alters antibiotic efficacy against the murine gut microbiota and attenuatesClostridioides difficilecolonization

Contact Info

Product

Resources

About