Dynamics of HSPA1A and redox status in the spermatozoa and fluid from different segments of goat epididymis

Kumar, Akhilesh; Yadav, Brijesh; Swain, Dilip Kumar; Anand, Mukul; Madan, Arun Kumar; Yadav, Raj Kumar; Kushawaha, Bhawna; Yadav, Sarita

doi:10.1007/s12192-020-01102-3

Cited by 5 publications

(4 citation statements)

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“…The epididymal cauda is an important place for sperm maturation and storage [ 25 ]. However, there is still a gap in the study of the epididymal cauda before and after sexual maturity in yaks [ 2 , 4 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity

Ding

Xiong

Wang

et al. 2023

Animals

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Epididymis development is the basis of male reproduction and is a crucial site where sperm maturation occurs. In order to further understand the epididymal development of yak and how to regulate sperm maturation, we conducted a multi-omics analysis. We detected 2274 differential genes, 222 differential proteins and 117 co-expression genes in the cauda epididymis of yak before and after sexual maturity by RNA-seq and proteomics techniques, which included TGFBI, COL1A1, COL1A2, COL3A1, COL12A1, SULT2B1, KRT19, and NPC2. These high abundance genes are mainly related to cell growth, differentiation, adhesion and sperm maturation, and are mainly enriched via extracellular matrix receptor interaction, protein differentiation and absorption, and lysosome and estrogen signaling pathways. The abnormal expression of these genes may lead to the retardation of epididymal cauda development and abnormal sperm function in yak. In conclusion, through single and combined analysis, we provided a theoretical basis for the development of the yak epididymal cauda, sperm maturation, and screening of key genes involved in the regulation of male yak reproduction.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity

Ding

Xiong

Wang

et al. 2023

Animals

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“…Two of the most highly upregulated microtubule-associated genes were Hspa1a and Cib1 . Hspa1a has been shown to be a key spermatozoa molecule that is essential for their structural and functional competence ( Kumar et al, 2020 ). Furthermore, Cib1 has been shown to be essential for mouse spermatogenesis ( Yuan et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

A muscle growth-promoting treatment based on the attenuation of activin/myostatin signalling results in long-term testicular abnormalities

Vaughan

Mitchell

Kretz

et al. 2021

Disease Models &Amp; Mechanisms

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Activin/Myostatin signalling acts to induce skeletal muscle atrophy in adult mammals by inhibiting protein synthesis as well as promoting protein and organelle turnover. Numerous strategies have been successfully developed to attenuate the signalling properties of these molecules which result in augmenting muscle growth. However, these molecules, in particular Activin, play major roles in tissue homeostasis in numerous organs of the mammalian body. We have recently shown that while the attenuation of Activin/Myostatin results in robust muscle growth, it also has detrimental impact on the testis. Here, we aimed to discover the long-term consequences of a brief period of exposure to molecules that promote muscle on the testis.We demonstrate that muscle hypertrophy promoted by a soluble Activin Type IIB ligand trap (sActRIIB) is a short-lived phenomenon. In stark contrast, short term treatment with sActRIIB results in immediate impact on the testis which persists after the sessions of the intervention. Gene array analysis identifies an expansion in aberrant gene expression over time in the testis initiated by a brief exposure to muscle growth promoting molecules. The impact on the testis results in decreased organ size as well as quantitative and qualitative impact on sperm. Finally, we have used a drug-repurposing strategy to exploit the gene expression data to identify a compound N6-methyladenosine, that may protect the testis from the impact of the muscle growth promoting regime. Taken together, this work shows potential long-term harmful effects of strategies aimed at promoting muscle growth by attenuating Activin/Myostatin signalling. Furthermore, we have identified a molecule that could in future be used to overcome the detrimental impact of sActRIIB treatment on the testis.

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“…Kumar et al [87] measured oxidative status in spermatozoa of sexually mature and healthy goats (2-3 years old). The sperm (10 × 10 8 spermatozoa/mg protein) was mechanically lysed by liquid nitrogen, centrifuged and the supernatant was used for the analysis.…”

Section: Ros Assay In Spermatozoamentioning

confidence: 99%

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

Pasciu

Nieddu

Sotgiu

et al. 2023

Animals

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The present review aims to provide an overview of the assay methods for the quantification of ROS and principal enzymatic antioxidants as biomarkers of oxidative stress in erythrocytes and spermatozoa of small domestic ruminants. A complete literature search was carried out in PubMed, Scopus and the World Wide Web using relevant keywords and focusing on the last five years (2018–2023). Among spectrophotometry, fluorometry and chemiluminescence, the most widely used method for ROS assay is fluorometry, probably because it allows to simultaneously assay several ROS, using different probes, with greater economic advantages. Regarding intracellular antioxidant enzymes, recent literature reports only spectrophotometric methods, many of which use commercial kits. The use of a less sensitive but cheapest method is suitable because both erythrocytes and spermatozoa samples are highly concentrated in domestic ruminant species. All methods considered in this review have been found to be appropriate; in general, the differences are related to their costs and sensitivity. Quantification of ROS and enzymatic antioxidant activity in erythrocytes and spermatozoa may find application in the study of the welfare and health status of small domestic ruminants for monitoring livestock production.

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Dynamics of HSPA1A and redox status in the spermatozoa and fluid from different segments of goat epididymis

Cited by 5 publications

References 58 publications

Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity

Comparative Analysis of Epididymis Cauda of Yak before and after Sexual Maturity

A muscle growth-promoting treatment based on the attenuation of activin/myostatin signalling results in long-term testicular abnormalities

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

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