Dynamics of high hydrostatic pressure resistance development in RpoS-deficient Escherichia coli

Gayán, Elisa; Wang, Zhiying; Salvador, Maika; Gänzle, Michael G.; Aertsen, Abram

doi:10.1016/j.foodres.2022.112280

Cited by 1 publication

(1 citation statement)

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“…The conservation of ggaR is high among Enterobacterales such as Shigella , Citrobacter , Salmonella , and Vibrio , suggesting that it plays an important role in adaptation to the gut environment. Recently, Gayán et al [ 47 ] reported that the lack of ggaR caused E. coli to gain high hydrostatic pressure (HHP) resistance, which was RpoS-independent. Glycogen metabolism is important for the acquisition of various stress tolerances ([ 5 ]; see also Figure 5 ), and HHP resistance may be a part of this.…”

Section: Discussionmentioning

confidence: 99%

Regulatory Role of GgaR (YegW) for Glycogen Accumulation in Escherichia coli K-12

Saito,

Kobayashi,

Hoshina

et al. 2024

Microorganisms

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Glycogen, the stored form of glucose, accumulates upon growth arrest in the presence of an excess carbon source in Escherichia coli and other bacteria. Chromatin immunoprecipitation screening for the binding site of a functionally unknown GntR family transcription factor, YegW, revealed that the yegTUV operon was a single target of the E. coli genome. Although none of the genes in the yegTUV operon have a clear function, a previous study suggested their involvement in the production of ADP-glucose (ADPG), a glycogen precursor. Various validation through in vivo and in vitro experiments showed that YegW is a single-target transcription factor that acts as a repressor of yegTUV, with an intracellular concentration of consistently approximately 10 molecules, and senses ADPG as an effector. Further analysis revealed that YegW repressed glycogen accumulation in response to increased glucose concentration, which was not accompanied by changes in the growth phase. In minimal glucose medium, yegW-deficient E. coli promoted glycogen accumulation, at the expense of poor cell proliferation. We concluded that YegW is a single-target transcription factor that senses ADPG and represses glycogen accumulation in response to the amount of glucose available to the cell. We propose renaming YegW to GgaR (repressor of glycogen accumulation).

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Section: Discussionmentioning

confidence: 99%