Dynamics of heavy metal accumulation in thalli of the epiphytic lichen Hypogymnia physodes

Михайлова, И. Н.; Sharunova, I. P.

doi:10.1134/s1067413608050068

Cited by 30 publications

(16 citation statements)

References 12 publications

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“…Such accumulation tendency was in accordance with the results of the studies with other heavy metals -Cd, Ni (Bačkor et al, 2010), Cu (Branquinho et al, 1999;). On the other hand, Cd was accumulated mainly in intracellular sites in Hypogymnia physodes thalli transplanted under conditions of chronic pollution (Mikhailova and Sharunova, 2008). Cd showed the highest affinity towards intracellular fraction also in moss Pleurozium schreberi samples (Virkutytė et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

Effects of Cadmium on Physiological Parameters of the LichenEvernia PrunastriandRamalina Fastigiata

Sujetovienė

Šliumpaitė

2013

E3S Web of Conferences

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Abstract. The aim of the study was to investigate in short term experiment the physiological effects and accumulation of cadmium in the lichens Evernia prunastri (L.) Ach. and Ramalina fastigiata (Pers.) Ach. Treatment of lichen thalli with 5, 25, 50 and 500 µM Cd solutions caused a significant decrease in chlorophyll a and b contents and production of membrane lipid peroxidation, expressed as MDA content. Severe negative physiological effects were observed at the highest Cd concentration (500 µM Cd). Cd content in treated lichens thalli increased gradually as Cd concentration increased in treatment solutions. It was concluded that Cd exposure causes physiological and oxidative stress, with higher damage to E. prunastri due to higher Cd content bounded at intracellular sites.

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Section: Resultsmentioning

confidence: 99%

Effects of Cadmium on Physiological Parameters of the LichenEvernia PrunastriandRamalina Fastigiata

Sujetovienė

Šliumpaitė

2013

E3S Web of Conferences

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“…The use of transplanted lichens allows assessing the response of organisms exposed to different atmospheric conditions including pollutants over fixed exposure period. This method also allows to determine threshold levels of toxic substances in a thallus (Mikhailova 2002). The transplant method is used in bioaccumulation (Garty et al 1996;Gonzalez and Pignata 1997;Canas and Pignata 1998;Zambrano and Nash 2000;Gonzalez et al 2003;Carreras et al 2005;Cloquet et al 2009;Oztetik and Cicek 2011;Aslan et al 2013).…”

Section: Biomonitoring Methods With Lichensmentioning

confidence: 97%

Monitoring Lichen as Indicators of Atmospheric Quality

Sujetovienė

2014

Recent Advances in Lichenology

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“…However, Hg (a class B element) can only be extracted with dimercaprol. The efficiency of extraction of extracellular Cd and Fe by EDTA has not been evaluated in any study, although the compound has been shown to be capable of extracting both elements in the studies in which it has been used (Mikhailova & Sharunova, 2008;Ferná ndez et al, 2004, respectively).…”

Section: Extractants Usedmentioning

confidence: 96%

“…For this purpose, a sequence of washing in boiling deionized water followed by total digestion was initially used , although in most later studies this was substituted by washing in dilute HNO 3 . In exceptional cases, some authors have used ethylenediaminetetraacetic acid (EDTA) as an intracellular extractant (Branquinho et al, 1999;Mikhailova & Sharunova, 2008).…”

Section: Description Of the Setmentioning

confidence: 99%

The sequential elution technique applied to cryptogams: a literature review

Pérez-Llamazares

Fernández

Carballeira

et al. 2011

Journal of Bryology

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The use of bryophytes and lichens as biomonitors of atmospheric contamination, particularly contamination by heavy metals, is a well-known and widely applied technique. Determination of the total concentrations in organisms is often used in such studies, but has some disadvantages that may be resolved by using the sequential elution technique (SET). This technique involves successive washing steps that enable quantification of the elements in the different cell compartments (intercellular, extracellular, and intracellular), and finally determination of the remaining fraction, i.e. particulate material. The key step in the SET is the correct extraction of the extracellular fraction, for which a suitable extractant must be used for each different element considered. We have found only seven studies that have focused on selecting suitable extractants, which may be metal cations or chelating agents. Ethylenediaminetetraacetic acid is presented as the most appropriate extracellular extractant for Al, Co, Cu, K, Mg, Pb, V, and Zn, for which it has been tested, and for Cd and Fe, for which it has not yet been tested (although it is known to be capable of extracting these elements). The only extractant that is capable of extracting extracellular Hg is dimercaprol. The technique has been used in laboratory studies and less often in field studies carried out in the surroundings of focal points of contamination. The elements analysed include nutrients (e.g. Ca, K, and Mg) and heavy metals (e.g. Cd, Zn, and Cu), usually associated with sources of emission of contaminants. Several problems have arisen in the application of the SET, some of which must be resolved. These include methodological problems (e.g. variability in the extraction process) and problems inherent in the SET itself, which are more difficult to resolve. Other techniques, such as histochemical techniques and electron microscopy with microanalysis, must therefore be used simultaneously with the SET to determine if the uptake of metal is only extracellular. At present, in light of the disadvantages of the quantification of the extracellular fraction, measurement of the intracellular fraction is presented as the best option because it is not affected by the metal burden in the particles, enables evaluation of environmental risks, better represents the average conditions of contamination, and enables better evaluation of phytotoxicity.

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Dynamics of heavy metal accumulation in thalli of the epiphytic lichen Hypogymnia physodes

Cited by 30 publications

References 12 publications

Effects of Cadmium on Physiological Parameters of the LichenEvernia PrunastriandRamalina Fastigiata

Effects of Cadmium on Physiological Parameters of the LichenEvernia PrunastriandRamalina Fastigiata

Monitoring Lichen as Indicators of Atmospheric Quality

The sequential elution technique applied to cryptogams: a literature review

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