Dynamics of follicular growth and atresia of large follicles during the ovarian cycle of the guinea pig: Fate of the degenerating follicles, a quantitative study

Logothetopoulos, John; Dorrington, Jennifer H.; Bailey, Dennis; Stratis, Mike

doi:10.1002/ar.1092430106

Cited by 23 publications

(22 citation statements)

References 17 publications

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“…Days of the estrous cycle followed from this point. Animals with at least 2 consecutive regular cycles of 16 d were sacrificed on Days 1, 4, 8, 12, and 16 (5 animals per day) (Garris and Mitchell, 1979;Logothetopoulos et al, 1995).…”

Section: Animals and Treatmentsmentioning

confidence: 99%

“…For guinea pigs, however, it has been difficult to provide uniform classification criteria because there are unique morphologic changes in the atretic process. The differentiation of theca cells (Logothetopoulos et al, 1995;Kasuya, 1997) is an example of one unique change. Fortunately, the process of follicular atresia was described in detail and divided into seven stages for the first time in 1995 (Logothetopoulos et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…The differentiation of theca cells (Logothetopoulos et al, 1995;Kasuya, 1997) is an example of one unique change. Fortunately, the process of follicular atresia was described in detail and divided into seven stages for the first time in 1995 (Logothetopoulos et al, 1995). This produced criteria for atresia that were, however, too complicated to observe and apply.…”

Section: Introductionmentioning

confidence: 99%

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Morphologic observation and classification criteria of atretic follicles in guinea pigs

Wang

Liu

Tian

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:There is a lack of appropriate classification criteria for the determination of atretic follicles in guinea pigs. In the present study, new criteria were established based on the latest morphologic criteria for cell death proposed by the Nomenclature Committee on Cell Death (NCCD) in 2009. Ovaries of guinea pigs were sampled on different stages of estrous cycle, and the morphologic observations of atretic follicles were investigated in serial sections. The results showed that the process of follicular atresia could be classified into four continuous stages: (1) the granulosa layer became loose, and some apoptotic bodies began to appear; (2) the granulosa cells were massively eliminated; (3) the theca interna cells differentiated; and (4) the residual follicular cells degenerated. In addition, the examination revealed that these morphologic criteria were accurate and feasible. In conclusion, this study provides new criteria for the classification of atretic follicles in guinea pigs, and this knowledge can inform future research in the area.

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Section: Animals and Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphologic observation and classification criteria of atretic follicles in guinea pigs

Wang

Liu

Tian

et al. 2010

J. Zhejiang Univ. Sci. B

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“…On the other hand, based on the results of quantitative light microscopic analysis, Logothetopoulos et al (1995) presumed that dead granulosa cells would be extirpated after autolysis, because there were no inflammatory cells such as granulocytes, lymphocytes or monocytes in the atretic follicules although ' fibroblastoid looking cells ' were seen. Moreover, the phenomenon of atresia, in which healthy granulosa cells phagocytose dead granulosa cells, has been reported in sheep (Hay et al 1976), dogs (Spanel-Borowski, 1981, mice (Byskov, 1979 ;Kuryszko, 1983 ;Kuryszko et al 1987 ;Watanabe, 1996) and rats (Peluso et al 1980 ;Takeo et al 1995).…”

Section: mentioning

confidence: 99%

Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice

et al. 2000

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We examined numerous ovarian follicles from 32-35 d virgin mice by transmission electron microscopy and light microscopic immunohistochemistry. No macrophages were seen, but various stages of apoptotic granulosa cells were encountered. Presumably a granulosa cell or its debris in an advanced stage of apoptosis was destined to be phagocytosed by adjacent normal-looking granulosa cells. Other granulosa cells of normal appearance were seen in the region of the zona pellucida in contact with and apparently phagocytosing atrophic oocytes. Such granulosa cells were characterised by the presence of gap junctions with other cells and frequently contained annular gap junctions in the cytoplasm. To confirm the lack of involvement of macrophages in the process of follicular atresia and elimination, specially prepared ovarian sections were incubated with antimouse macrophage monoclonal antibodies (F4\80, Mac-1, Mac-2). None of the follicles examined showed positive immunoreactivity with these antibodies. Atretic follicles may shrink and eventually disappear from the ovary as a result of repeated apoptosis and phagocytosis by granulosa cells. There is no evidence for the presence or involvement of macrophages in the atretic follicles, at least in prereproductive mice as examined.

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“…These morphological and biochemical changes that occur during apoptosis in ovarian follicles are similar to apoptotic changes occurring in other tissues and cells (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15).…”

mentioning

confidence: 68%

Confocal laser scanning microscopy of whole mouse ovaries: Excellent morphology, apoptosis detection, and spectroscopy

Zucker

Jeffay

2006

Cytometry Pt A

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Background:Ovaries consist of numerous follicles, oocytes, and granulosa cells in different stages of development. Many of these follicles will undergo an apoptotic process during the lifetime of the animal. By using proper tissue preparation methods, the events within the whole ovary can be observed by using 3D confocal microscopy.Methods:Whole ovaries were stained with LysoTracker Red (LT), fixed with 4% paraformaldehyde (PF) and 1% glutaraldehyde (Glut), dehydrated with methanol (MEOH), and cleared with benzyl alcohol and benzyl benzoate (BABB). Using this tissue preparation technique, the ovary becomes relatively transparent, allowing its morphology to be observed with confocal microscopes. A spectral imaging system (PARISS) located on a conventional microscope was used to interpret the LT dye spectra and fixation products in the tissues with different excitation wavelengths.Results:Apoptosis in the follicle was detected as clusters of intensely stained granulosa cells located in close proximity to the oocytes. The fixation with Glut and PF preserved morphological details, increased tissue fluorescence, thus increased the signal to noise of the background image.Conclusions:Thick tissues can be imaged after they are properly stained, aldehyde fixed, and BABB cleared. LT intensely stained single cells or clusters of apoptotic cells in the follicles and the nucleolus. Spectral differences between LT as an indicator of apoptosis and Glut‐PF fixation was used to visualize ovarian morphology and apoptosis. The PARISS spectrophotometer revealed spectral peaks for LT at 609.6 nm and for Glut‐PF at 471.3 nm. The proper use of the spectra from these fluorescence molecules is the foundation for high quality morphological images of apoptosis. By sequentially imaging the two probes with a 488 nm laser and a 543/568 nm laser, there was a reduction in fluorescent cross talk and an increase in image quality. © 2006 International Society for Analytical Cytology

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Dynamics of follicular growth and atresia of large follicles during the ovarian cycle of the guinea pig: Fate of the degenerating follicles, a quantitative study

Cited by 23 publications

References 17 publications

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice

Confocal laser scanning microscopy of whole mouse ovaries: Excellent morphology, apoptosis detection, and spectroscopy

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