Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Deenarn, Pacharawan; Tobwor, Punsa; Leelatanawit, Rungnapa; Wongtriphop, Somjai; Khudet, Jutatip; Karoonuthaisiri, Nitsara; Vichai, Vanicha; Wimuttisuk, Wananit

doi:10.1530/rep-18-0143

Cited by 2 publications

(5 citation statements)

References 52 publications

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“…These genes are part of the fatty acid beta-oxidation pathway, which metabolizes fatty acids for ATP production (Houten & Wanders 2010). Our data correspond with results from other studies that fatty acid beta-oxidation is up-regulated in ovaries during ovarian development due to increased energy requirements (Paczkowski et al 2013, Dunning et al 2014, Deenarn et al 2018.…”

Section: Effects Of Shrimp Diet and Ovarian Maturation On Fatty Acid Regulatory Genessupporting

confidence: 90%

“…Shrimp were fed with B2 commercial feed pellets (Charoen Pokphand Food Public Company, Ltd., Bangkok, Thailand) and maintained in seawater at 30 ppt and 27°C. Ammonium, nitrite and nitrate levels, alkalinity and pH were monitored and adjusted twice a week as previously described (Deenarn et al 2018).…”

Section: Shrimp Rearing and Feed Experimentsmentioning

confidence: 99%

“…Primer sequences for prostaglandin biosynthesis and fatty acid regulatory genes are provided in Supplementary Table 1. Melt curve analysis for each primer pair was performed as previously described (Deenarn et al 2018). Amounts of target gene transcripts relative to that of elongation factor 1α (EF1A) were obtained using the standard curve method.…”

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

“…Polychaete consumption has been shown to increase the amounts of ARA in hepatopancreases of M. japonicus (Binh et al 2008). Increasing lipid content in crustacean hepatopancreases correlated with transcriptional changes in the fatty acid regulatory pathway, including beta-oxidation, metabolism, synthesis and transport in the Chinese mitten crab Eriocheir sinensis, the swimming crab Portunus trituberculatus, the Pacific white shrimp Penaeus vannamei and P. monodon (Chen et al 2015, Wei et al 2017, Deenarn et al 2018, Yuan et al 2019.…”

Section: Introductionmentioning

confidence: 98%

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Polychaete consumption increased prostaglandin biosynthesis in female Penaeus monodon

et al. 2020

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The polychaete Perinereis nuntia is preferred over commercial feed pellets for boosting ovarian maturation of the female black tiger shrimp Penaeus monodon. High levels of prostaglandins in polychaetes are believed to enhance shrimp ovarian development. However, the impact of polychaete feeding on shrimp prostaglandin biosynthesis and fatty acid regulatory pathways have yet to be investigated. As polychaetes contain higher levels of arachidonic acid (ARA), eicosapentaenoic acid (EPA), prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) than feed pellets, we examined the effects of polychaete feeding alone and in combination with eyestalk ablation on shrimp hepatopancreases and ovaries. Shrimp fed with polychaetes contained higher levels of EPA, PGE2 and PGF2α in hepatopancreases than those of pellet-fed shrimp. Similarly, higher levels of ARA and higher transcription levels of cyclooxygenase (COX) and prostaglandin F synthase (PGFS) were detected in ovaries of polychaete-fed shrimp compared to those of pellet-fed shrimp. The combination of polychaete-feeding and eyestalk ablation, commonly practiced to induce ovarian development, increased levels of ARA and EPA and transcription levels of COX in hepatopancreases and ovaries of polychaete-fed shrimp compared to those of pellet-fed shrimp. In ovaries, prostaglandin biosynthesis gene transcripts were induced by polychaete feeding while transcriptional levels of fatty acid regulatory genes were regulated by shrimp feed and eyestalk ablation. Our findings not only elucidate the effects of polychaete consumption on shrimp prostaglandin biosynthesis and fatty acid regulatory pathways during larvae production, but also suggests that high levels of dietary ARA, EPA and prostaglandins are essential during P. monodon ovarian development.

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Section: Effects Of Shrimp Diet and Ovarian Maturation On Fatty Acid Regulatory Genessupporting

confidence: 90%

Section: Shrimp Rearing and Feed Experimentsmentioning

confidence: 99%

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Polychaete consumption increased prostaglandin biosynthesis in female Penaeus monodon

et al. 2020

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“…Given polychaete's richness in PUFAs, it is not surprising to find several genes involved in fatty acid regulatory networks differentially specifically induced in the polychaete-fed group, especially after the eyestalk ablation. Previously, genes in the fatty acid regulation pathways have been shown to be highly expressed in mature ovaries of P. monodon fed with polychaetes after eyestalk ablation 38 . Fatty acids and dietary lipids are important for vitellogenesis as yolk proteins are composed mainly of lipoglycoproteins 39 .…”

Section: Synergistic Effects From Diets and Eyestalk Ablationmentioning

confidence: 99%

Transcriptome analyses reveal the synergistic effects of feeding and eyestalk ablation on ovarian maturation in black tiger shrimp

Sittikankaew

Pootakham

Sonthirod

et al. 2020

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Unilateral eyestalk ablation in the female black tiger shrimp Penaeus monodon is commonly employed to induce ovarian maturation. However, the importance of complementing this practice with the provision of live feed supplement (such as polychaetes) has not been emphasized in previous studies. Indeed, it has been less emphasized that female broodstock must be fed with live feeds such as polychaetes for this practice to be effective. While the effects of eyestalk ablation have been thoroughly studied in various aspects, the synergistic effects of feeding with live feeds and the ablation have never been elucidated at a transcriptome-wide level. With recent advances in the next-generation sequencing platforms, it is now possible to investigate the effects of eyestalk ablation and live feeds at the transcriptomic levels. This study employed both short-read Illumina RNA sequencing and long-read Pacific Biosciences (PacBio) isoform sequencing (Iso-seq) to generate the first high-quality ovarian reference transcriptome in P. monodon. This novel assembly allowed us to dissect the effects of feeds and eyestalk ablation and reveal their synergistic effects at the transcriptomic level through the regulation of important genes involved in fatty acid regulation, energy production, and hormonemediated oocyte maturation pathways. The synergistic effects between the polychaete feeding and the eyestalk ablation in the process of ovarian maturation in black tiger shrimp suggest that without having proper nutrients from the polychaetes, female broodstock might not be ready to develop its ovary. However, even with proper nutrients, the eyestalk ablation is still necessary to perhaps manipulate the female endocrine of the black tiger shrimp. These findings shed the light on molecular mechanisms and key molecular pathways that lead to successful ovarian maturation. The black tiger shrimp (Penaeus monodon) is the second most widely cultured shrimp species in the world (FAO 2016). Albeit high demand worldwide, the shrimp production level remains stagnant mainly due to the outbreak of diseases and lack of selective breeding programs. The future success of the shrimp industry will depend upon increasing supplies of healthy, high-quality seed for stocking ponds and decreasing reliance on wild broodstock, which might potentially be a disease carrier and have seasonal variation 1. To overcome these challenges, it is vital to be able to enhance reproductive maturation of captive broodstock. Ovarian maturation is a well-orchestrated and complex molecular process that requires the regulation of large sets of genes to ensure proper oocyte development for embryogenesis. In penaeid shrimp, the process can be categorized into four stages: (I) previtellogenesis, (II) vitellogenesis, (III) early cortical rod, and (IV) late cortical rod 2. Numerous factors and methods have been investigated to improve reproductive maturation in female black tiger shrimp. Dietary choices have been shown to be important for the maturation. Although nutritional

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Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Cited by 2 publications

References 52 publications

Polychaete consumption increased prostaglandin biosynthesis in female Penaeus monodon

Polychaete consumption increased prostaglandin biosynthesis in female Penaeus monodon

Transcriptome analyses reveal the synergistic effects of feeding and eyestalk ablation on ovarian maturation in black tiger shrimp

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