Thermophysiological comfort properties of polyester elastane-plated fabric have been studied by varying the polyester filament shape factor, elastane linear density, and fabric loop length. Thermal and evaporative resistances were measured using sweating guarded hot plate. Absorption and wicking properties were measured using M/K GATS. Moisture management properties were measured by moisture management tester. Multilinear regression equations were calculated for all the properties by using generalized linear model (GLM). The hypothesis tests were performed on estimated model (GLM) and the probability values (p-values) were calculated for each variable. Most of the heat and moisture transmission properties were found to be significantly affected by all three variables (i.e. filament shape factor, elastane linear density, and fabric loop length) at 95% level of significance.