Dynamics of different arachidonic acid orientations bound to prostaglandin endoperoxide synthases

Sachett, Liana Guimarães; Verli, Hugo

doi:10.1016/j.ejmech.2011.08.016

Cited by 3 publications

(1 citation statement)

References 44 publications

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“…is the rate-limited enzyme for converting AA into PGs, which are an essential class of inflammatory mediators (Sachett and Verli, 2011;Akasaka and Ruan, 2016). In particular, COX-2, an isozyme of COX, is an inducible enzyme that is produced rapidly by stimulation, and then catalyzes the synthesis of PGs relating to the inflammatory response (Johnsen et al, 2004;Akasaka and Ruan, 2016).…”

Section: Discussionmentioning

confidence: 99%

Identification of a Pharmacological Biomarker for the Bioassay-Based Quality Control of a Thirteen-Component TCM Formula (Lianhua Qingwen) Used in Treating Influenza A Virus (H1N1) Infection

Gao¹,

Niu

Wei

et al. 2020

Front. Pharmacol.

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As chemical analysis for quality control (QC) of traditional Chinese medicine (TCM) formula is difficult to guarantee the effectiveness, a bioassay method that combines QC with evaluation of therapeutic effects has been developed to assess the TCM quality. Here, we chose a thirteen-component TCM formula, Lianhua Qingwen capsule (LHQW), as a representative sample, to explore the pivotal biomarkers for a bioassay and to investigate close association between QC and pharmacological actions. Initially, our results showed that chemical fingerprinting could not effectively distinguish batches of LHQW. Pharmacological experiments indicated that LHQW could treat influenza A virus (H1N1) infection in the H1N1 mouse model, as claimed in clinical trials, by improving pathologic alterations and bodyweight loss, and decreasing virus replication, lung lesions and inflammation. Furthermore, by using serum metabolomics analysis, we identified two important metabolites, prostaglandin F 2a and arachidonic acid, and their metabolic pathway, arachidonic acid metabolism, as vital indicators of LHQW in treatment of influenza. Subsequently, macrophages transcriptomics highlighted the prominent role of cyclooxygenase-2 (COX-2) as the major rate-limiting enzyme in the arachidonic acid metabolism pathway. Finally, COX-2 was validated by in vivo gene expression and in vitro enzymatic activity with 43 batches of LHQW as a viable pharmacological biomarker for the

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Section: Discussionmentioning

confidence: 99%

Identification of a Pharmacological Biomarker for the Bioassay-Based Quality Control of a Thirteen-Component TCM Formula (Lianhua Qingwen) Used in Treating Influenza A Virus (H1N1) Infection

Gao¹,

Niu

Wei

et al. 2020

Front. Pharmacol.

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Energetics of the biosynthesis of prostanes from arachidonate

et al. 2015

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Producing Glucose 6-Phosphate from Cellulosic Biomass

Klesmith

Whitehead

Jarboe

et al. 2015

Journal of Biological Chemistry

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Background: Levoglucosan kinase converts the cellulosic biomass pyrolysis product levoglucosan to the common metabolite glucose 6-phosphate.Results: Crystallographic structures demonstrate two metal binding sites and an alternate binding arrangement for levoglucosan. Conclusion:The unusual mode of levoglucosan binding provides a rationale for the high K m of LGK for its substrate. Significance: These results provide a greater understanding of the underlying biochemistry of anhydrosugar processing.

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Dynamics of different arachidonic acid orientations bound to prostaglandin endoperoxide synthases

Cited by 3 publications

References 44 publications

Identification of a Pharmacological Biomarker for the Bioassay-Based Quality Control of a Thirteen-Component TCM Formula (Lianhua Qingwen) Used in Treating Influenza A Virus (H1N1) Infection

Identification of a Pharmacological Biomarker for the Bioassay-Based Quality Control of a Thirteen-Component TCM Formula (Lianhua Qingwen) Used in Treating Influenza A Virus (H1N1) Infection

Energetics of the biosynthesis of prostanes from arachidonate

Producing Glucose 6-Phosphate from Cellulosic Biomass

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