Dynamics of CD62L/CD45RB CD4+ and CD8+ lymphocyte subsets in hepatic and splenic tissues during murine visceral leishmaniasis

Gomes-Pereira, Sandra; Rodrigues, Olı́via Roos; Santos‐Gomes, Gabriela

doi:10.1016/j.imlet.2004.06.005

Cited by 14 publications

(8 citation statements)

References 39 publications

(29 reference statements)

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“…In a previous work, our group observed slight variations in the CD4 + memory subsets CD62L low /CD45RB low of splenic lymphocytes in mice of both ''cure'' and ''non-cure'' phenotype infected with L. infantum (Gomes-Pereira et al, 2004b). Together, these results indicate that perhaps CD45RB may not be a suitable marker for Treg subsets that expand during L. infantum BALB/c infection and that cells with this phenotype may not be directly involved in suppressing effector immune response.…”

Section: Article In Pressmentioning

confidence: 84%

Identification of regulatory T cells during experimental Leishmania infantum infection

et al. 2009

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Section: Article In Pressmentioning

confidence: 84%

Identification of regulatory T cells during experimental Leishmania infantum infection

et al. 2009

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“…Previous reports have shown that upon activation of T effs , surface CD62L is usually decreased [35-37]. However, T regs normally maintain CD62L expression and functional phenotype [38].…”

Section: Discussionmentioning

confidence: 99%

Signal peptide cleavage is essential for surface expression of a regulatory T cell surface protein, leucine rich repeat containing 32 (LRRC32)

et al. 2011

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BackgroundElevated numbers of regulatory T cells (Tregs) have been implicated in certain cancers. Depletion of Tregs has been shown to increase anti-tumor immunity. Tregs also play a critical role in the suppression of autoimmune responses. The study of Tregs has been hampered by a lack of adequate surface markers. Leucine Rich Repeat Containing 32 (LRRC32), also known as Glycoprotein A Repetitions Predominant (GARP), has been postulated as a novel surface marker of activated Tregs. However, there is limited information regarding the processing of LRRC32 or the regulatory phenotype and functional activity of Tregs expressing LRRC32.ResultsUsing naturally-occurring freshly isolated Tregs, we demonstrate that low levels of LRRC32 are present intracellularly prior to activation and that freshly isolated LRRC32+ Tregs are distinct from LRRC32- Tregs with respect to the expression of surface CD62L. Using LRRC32 transfectants of HEK cells, we demonstrate that the N-terminus of LRRC32 is cleaved prior to expression of the protein at the cell surface. Furthermore, we demonstrate using a construct containing a deleted putative signal peptide region that the presence of a signal peptide region is critical to cell surface expression of LRRC32. Finally, mixed lymphocyte assays demonstrate that LRRC32+ Tregs are more potent suppressors than LRRC32- Tregs.ConclusionsA cleaved signal peptide site in LRRC32 is necessary for surface localization of native LRRC32 following activation of naturally-occurring freshly-isolated regulatory T cells. LRRC32 expression appears to alter the surface expression of activation markers of T cells such as CD62L. LRRC32 surface expression may be useful as a marker that selects for more potent Treg populations. In summary, understanding the processing and expression of LRRC32 may provide insight into the mechanism of action of Tregs and the refinement of immunotherapeutic strategies aimed at targeting these cells.

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“…Monoclonal antibodies against a canine CD45 homologue, canine phagocytes, canine CD45RA and canine CD45RB antigens were used. Naïve T cells, helper T cells secreting IFN-g and a wide range of B cells express the high molecular weight (220-240 kDa) CD45RA antigen (Cobbold and Metcalfe, 1994;Zuckermann et al, 1998), while the CD45RB antigen has been associated to CD4 + T cells that had been activated in the course of a viral (Varga and Welsh, 1996) or protozoan (Gomes-Pereira et al, 2004) infection. Fine-needle aspirations, as performed in the work described below, can allow repeated (Barrouin-Melo et al, 2006) evaluations of splenic changes in dogs, contrasting with the surgical or necropsy procedures used in most reports on the pathological alterations of CVL.…”

Section: Introductionmentioning

confidence: 99%

A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis

Barrouin‐Melo

Larangeira

Santos

et al. 2006

Veterinary Immunology and Immunopathology

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A method for the evaluation of splenic cellularity using samples collected by fine-needle aspirative biopsy was standardized in this work. The procedure includes erythrocyte lysing, preparation of cytospin films and staining by histochemical and immunocytochemical techniques. The cellular profiles of spleen preparations were compared with those observed in peripheral blood samples subjected to the same procedure. Two groups were compared, one consisting of 14 healthy uninfected and the other of 15 polysymptomatic Leishmania chagasi/infantum-infected dogs, from an endemic area for visceral leishmaniosis. Cell populations were identified by conventional hematoxilin-eosin and Wright' stainings, and by immunocytochemistry using monoclonal antibodies against canine CD45RA and CD45RB, phagocytes and a pan-leukocyte antigen. Larger neutrophil (P < 0.0001) and monocyte/macrophage (P = 0.0036) relative counts and lower lymphocyte relative counts (P < 0.0001) were found in the spleen, and not in the blood, of the animals with leishmaniosis than in those of the healthy animals. The proportions of CD45RB+ cells were higher, and of CD45RA+ cells were lower, both in the spleen and in the blood of animals with leishmaniosis than in those of healthy dogs (P < 0.05). Additionally, hematoxilin-eosin-stained cytospins of spleen aspirates from Leishmania-infected animals permitted the easy visualization of amastigote forms inside phagocytes, under light microscopy.

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Dynamics of CD62L/CD45RB CD4+ and CD8+ lymphocyte subsets in hepatic and splenic tissues during murine visceral leishmaniasis

Cited by 14 publications

References 39 publications

Identification of regulatory T cells during experimental Leishmania infantum infection

Identification of regulatory T cells during experimental Leishmania infantum infection

Signal peptide cleavage is essential for surface expression of a regulatory T cell surface protein, leucine rich repeat containing 32 (LRRC32)

A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis

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