Research in natural
products (NPs) has gained interest as drug
developers turn to nature to combat problems with drug resistance,
drug delivery, and emerging diseases. Whereas NPs offer a tantalizing
source of new pharmacologically active compounds, their structural
complexity presents a challenge for analytical characterization and
organic synthesis. Of particular concern is the characterization of
cyclic-, polycyclic-, or macrocyclic compounds. One example of endogenous
compounds as inspiration for NP development are cobalamins, like vitamin
B12. An example of exogenous NPs is the class of macrolides
that includes erythromycin. Both classes of macrocycles feature analogues
with a range of modifications on their macrocyclic cores, but because
of their cyclic nature, they are generally resistant to fragmentation
by collision-induced dissociation (CID). In the present work, charge-transfer
dissociation (CTD) was employed, with or without supplemental collisional
activation, to produce radical-driven, high-energy fragmentation products
of different macrocyclic precursors. With the assistance of collisional
activation of CTnoD products, CTD frequently cleaved two covalent
bonds within the macrocycle cores to reveal rich, informative spectra
that helped identify sites of modification and resolve structural
analogues. In a third example of macrocycle fragmentation, CTD enabled
an impurity in a biological sample to be characterized as a cyclic
polymer of nylon-6,6. In each example, CTD spectra are starkly different
from CID and are highly reminiscent of other high-energy fragmentation
techniques like extreme ultraviolet dissociative photoionization (XUV-DPI)
and electron ionization-induced dissociation (EID). The results indicate
that CTD-MS is a useful tool for the characterization of natural and
synthetic macrocycles.