Dynamics of branched tissue assembly

Manivannan, Sriram; Nelson, Celeste M.

doi:10.1186/scrt133

Cited by 9 publications

(6 citation statements)

References 49 publications

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“…To clarify this process, several models have been used [5] – [8] . For instance, skin appendages including the mammary gland develop through complex reciprocal interactions between mesenchyme and epithelium [9] , [10] .…”

Section: Introductionmentioning

confidence: 99%

From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time

et al. 2014

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BackgroundMammary gland morphogenesis involves ductal elongation, branching, and budding. All of these processes are mediated by stroma - epithelium interactions. Biomechanical factors, such as matrix stiffness, have been established as important factors in these interactions. For example, epithelial cells fail to form normal acinar structures in vitro in 3D gels that exceed the stiffness of a normal mammary gland. Additionally, heterogeneity in the spatial distribution of acini and ducts within individual collagen gels suggests that local organization of the matrix may guide morphogenesis. Here, we quantified the effects of both bulk material stiffness and local collagen fiber arrangement on epithelial morphogenesis.ResultsThe formation of ducts and acini from single cells and the reorganization of the collagen fiber network were quantified using time-lapse confocal microscopy. MCF10A cells organized the surrounding collagen fibers during the first twelve hours after seeding. Collagen fiber density and alignment relative to the epithelial surface significantly increased within the first twelve hours and were a major influence in the shaping of the mammary epithelium. The addition of Matrigel to the collagen fiber network impaired cell-mediated reorganization of the matrix and increased the probability of spheroidal acini rather than branching ducts. The mechanical anisotropy created by regions of highly aligned collagen fibers facilitated elongation and branching, which was significantly correlated with fiber organization. In contrast, changes in bulk stiffness were not a strong predictor of this epithelial morphology.ConclusionsLocalized regions of collagen fiber alignment are required for ductal elongation and branching suggesting the importance of local mechanical anisotropy in mammary epithelial morphogenesis. Similar principles may govern the morphology of branching and budding in other tissues and organs.

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Section: Introductionmentioning

confidence: 99%

From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time

et al. 2014

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“…Additionally, utilizing three-dimensional culture models, stem cells have been shown to have the capacity of tissue formation and organ reconstruction [20]. Also, it has been proposed that apoptotic/dying stem cells exhibit distinctive immunosuppressive properties [21].…”

Section: Introductionmentioning

confidence: 99%

“…Not only has growing evidence demonstrated that stem cells possess the intrinsic capacity of immunomodulation [ 15 - 17 ], but stem cell therapy has also been shown to attenuate inflammation and immune responses [ 15 - 19 ] and augment wound healing cells to favor tissue regeneration and inhibit fibrotic tissue formation [ 19 ]. Additionally, utilizing three-dimensional culture models, stem cells have been shown to have the capacity of tissue formation and organ reconstruction [ 20 ]. Also, it has been proposed that apoptotic/dying stem cells exhibit distinctive immunosuppressive properties [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Apoptotic adipose-derived mesenchymal stem cell therapy protects against lung and kidney injury in sepsis syndrome caused by cecal ligation puncture in rats

et al. 2013

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IntroductionWe tested the hypothesis that apoptotic adipose-derived mesenchymal stem cells (A-ADMSC) are superior to healthy (H)-ADMSC in attenuating cecal ligation puncture (CLP)-induced sepsis-mediated lung and kidney injuries.MethodsAdult male rats divided into group 1 (sham controls), group 2 (CLP), group 3 [CLP + H-ADMSC administered at 0.5, 6, and 18 hours after CLP], and group 4 [CLP + A-ADMSC administered as in group 3] were sacrificed 72 hours after CLP with blood, lung, and kidney collected for studies.ResultsWhite blood cell (WBC) count, circulating TNF-α and creatinine levels were higher in groups 2 and 3 than in groups 1 and 4 (all P < 0.001). Kidney and lung damage scores were highest in group 2, lowest in group 1, significantly higher in group 3 than in group 4 (all P < 0.0001). Protein expressions of inflammatory (ICAM-1, MMP-9, TNF-α, NF-κB), oxidative, and apoptotic (Bax, caspase-3, PARP) biomarkers were higher in groups 2 and 3 than groups 1 and 4, whereas anti-apoptotic (Bcl-2) and mitochondrial integrity (cytochrome-C) biomarkers were lower in groups 2 and 3 than in groups 1 and 4 (all P < 0.001). Expressions of anti-oxidant biomarkers at protein (GR, GPx, NQO-1, HO-1) and cellular (GR, GPx) levels were highest in group 4 (all P < 0.001). The number of inflammatory cells (CD3+) in lungs and levels of DNA damage marker (γ-H2AX) in kidneys were higher in groups 2 and 3 than in groups 1 and 4 (all P < 0.001).ConclusionsA-ADMSC therapy was superior to H-ADMSC therapy in protecting major organs from damage in rats with CLP-induced sepsis syndrome.

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“…151,152 The neural guidance cue netrin-1 is also necessary for mammary branch formation, for it maintains connections between the preluminal cells to the cap cells in the terminal end buds of the mammary branches. 153,154 Therefore, the four cell outgrowth behaviors we have identified due to the presence of common genes between C. elegans outgrowth systems, are also behaviors used by outgrowth in other organisms.…”

Section: Behaviors Undertaken By Outgrowing Cellsmentioning

confidence: 94%

Non-neuronal cell outgrowth inC. elegans

Ghosh

Vetrone

Sternberg

2017

Worm

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Cell outgrowth is a hallmark of some non-migratory developing cells during morphogenesis. Understanding the mechanisms that control cell outgrowth not only increases our knowledge of tissue and organ development, but can also shed light on disease pathologies that exhibit outgrowth-like behavior. is a highly useful model for the analysis of genes and the function of their respective proteins. In addition, also has several cells and tissues that undergo outgrowth during development. Here we discuss the outgrowth mechanisms of nine different cells and tissues. We specifically focus on how these cells and tissues grow outward and the interactions they make with their environment. Through our own identification, and a meta-analysis, we also identify gene families involved in multiple cell outgrowth processes, which defined potential core components of cell outgrowth, as well as identify a potential stepwise cell behavioral cascade used by cells undergoing outgrowth.

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Dynamics of branched tissue assembly

Cited by 9 publications

References 49 publications

From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time

From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time

Apoptotic adipose-derived mesenchymal stem cell therapy protects against lung and kidney injury in sepsis syndrome caused by cecal ligation puncture in rats

Non-neuronal cell outgrowth inC. elegans

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