Dynamics of bacterial community in Tonle Sap Lake, a large tropical flood-pulse system in Southeast Asia

Ung, Porsry; Peng, Chanthol; Yuk, Sokunsreiroat; Tan, Reasmey; Ann, Vannak; Miyanaga, Kazuhiko; Tanji, Yasunori

doi:10.1016/j.scitotenv.2019.01.351

Cited by 18 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to this natural phenomenon, Tonle Sap Lake is of great significance to flood control, economic development, and the ecological value of Cambodia. At present, the researches of Tonle Sap Lake mainly focus on biodiversity [25][26][27], water quality [28][29][30], hydrological characteristics [31][32][33], social and economic conditions [34,35].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Variation Characteristics and Driving Factors of Tonle Sap Lake’s Surface Water Temperature from 2001 to 2018

Pan¹,

Yang²

2021

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

Lake surface water temperature (LSWT) has a significant impact on aquatic ecosystem. This study aimed to reveal the variations and driving factors of LSWT in Tonle Sap Lake, the largest freshwater lake in South Asia. For this purpose, the datasets of LSWT-day, atmospheric temperature (AT), lake surface area (LSA), watershed land use and cover change (WLUCC) were extracted based on MODIS products and AT images by remote sensing technology. Then, through GIS geospatial analysis technology and mathematical statistical methods, the spatial-temporal variations of LSWT-day, AT, LSA and WLUCC were analyzed, and the relationships between LSWT-day and AT, LSA, WLUCC were further discussed. Results showed that: 1) from 2001 to 2018, the annual mean LSWT-day and AT showed a significant warming trend. AT was a main driving factor affecting LSWT in Tonle Sap Lake.2) LSA showed a slightly decreasing tendency and had an obvious negative correlation with LSWT-day, which was an important factor affecting LSWT. 3) WLUCC had undergone drastic changes and its influence on LSWT could not be ignored. In summary, this paper fills the gap of the long-term series of LSWT research in Tonle Sap Lake and enhances the understanding of LSWT changing mechanism for similar lakes.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of Variation Characteristics and Driving Factors of Tonle Sap Lake’s Surface Water Temperature from 2001 to 2018

Pan¹,

Yang²

2021

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

show abstract

“…The whole process of DNA extraction was performed on a bacteria-free workbench with UV irradiation before operation. DNA quality and concentration were measured by gel electrophoresis and a Nanodrop spectrophotometer (Nanodrop 2000, Thermo Scientific, Wilmington, USA), respectively [26]. Extracted DNA was stored at −20 • C and then sent to Shanghai Majorbio Bio-pharm Technology Company (China) for sequencing.…”

Section: Genomic Dna Extraction and High-throughput Sequencingmentioning

confidence: 99%

“…The main characteristic of reclaimed water was that it contained the highest abundance of Proteobacteria. Proteobacteria have been reported as the most widespread bacteria in the active sludge community of sewage treatment plants [48], and many species of this phylum pose a potential risk to human health [26]. The predominant phyla (generally, in this study, predominant phyla had a relative abundance >10.00%) of natural water (NW) were Cyanobacteria (29.01%), Bacteroidetes (27.84%), Proteobacteria (27.36%) and Actinobacteria (12.63%) ( Figure 4b).…”

Section: Watermentioning

confidence: 99%

Changes in Microbial Community Structures under Reclaimed Water Replenishment Conditions

Sun

Wang

et al. 2020

IJERPH

View full text Add to dashboard Cite

Using reclaimed water as a resource for landscape water replenishment may alleviate the major problems of water resource shortages and water environment pollution. However, the safety of the reclaimed water and the risk of eutrophication caused by the reclaimed water replenishment are unclear to the public and to the research community. This study aimed to reveal the differences between natural water and reclaimed water and to discuss the rationality of reclaimed water replenishment from the perspective of microorganisms. The microbial community structures in natural water, reclaimed water and natural biofilms were analyzed, and the community succession was clarified along the ecological niches, water resources, fluidity and time using 16S rRNA gene amplicon sequencing. Primary biofilms without the original community were added to study the formation of microbial community structures under reclaimed water acclimation. The results showed that the difference caused by ecological niches was more than those caused by the fluidity of water and different water resources. No significant difference caused by the addition of reclaimed water was found in the microbial diversity and community structure. Based on the results of microbial analysis, reclaimed water replenishment is a feasible solution that can be used for supplying river water.

show abstract

“…Therefore, this study aimed to isolate, screen, and cultivate aquatic cellulolytic bacteria from a complex and unique freshwater ecosystem, namely Tonle Sap Lake. It is the largest freshwater lake in Southeast Asia and is located in the central part of Cambodia [40,41]. This lake is known as one of the most biodiverse and productive freshwater ecosystems in the world [40,42].…”

Section: Introductionmentioning

confidence: 99%

Diversity and Activity of Aquatic Cellulolytic Bacteria Isolated from Sedimentary Water in the Littoral Zone of Tonle Sap Lake, Cambodia

Chantarasiri

2021

Water

View full text Add to dashboard Cite

Tonle Sap Lake is the largest freshwater lake in Southeast Asia, and it is regarded as one of the most biodiverse freshwater ecosystems in the world. Studies concerning aquatic cellulolytic bacteria from Tonle Sap Lake remain scarce. Cellulolytic bacteria and their cellulases play a vital role in the biogeochemical cycles of lake environments, and their application in biotechnological industries is likewise an important component of their usage. This study aimed to assess the isolation, genetic identification, bioinformatic analyses, and activity characterization of aquatic cellulolytic bacteria. The cellulolytic bacteria isolated from sedimentary water samples in the littoral zone of the lake belong to the genera Aeromonas, Bacillus, and Exiguobacterium. Several isolated aquatic bacteria were designated as rare cellulolytic microbes. Remarkably, B. mojavensis strain REP303 was initially evidenced by the aquatic cellulolytic bacterium in freshwater lake ecosystems. It was considered a highly active cellulolytic bacterium capable of creating a complete cellulase system involving endoglucanase, exoglucanase, and β-glucosidase. The encoded endoglucanase belongs to the glycosyl hydrolase family 5 (GH5), with a carboxymethylcellulase (CMCase) activity of 3.97 ± 0.05 U/mL. The optimum temperature and pH for CMCase activity were determined to be 50 °C at a pH of 7.0, with a stability range of 25–55 °C at a neutral pH of 7.0–8.0. The CMCase activity was enhanced significantly by Mn2+ and was inhibited considerably by EDTA and ethyl-acetate. In conclusion, this study is the first to report data concerning aquatic cellulolytic bacteria isolated from the littoral zone of Tonle Sap Lake. A novel strain of isolated cellulolytic B. mojavensis could be applied in various cellulose-based industries.

show abstract

Dynamics of bacterial community in Tonle Sap Lake, a large tropical flood-pulse system in Southeast Asia

Cited by 18 publications

References 35 publications

Analysis of Variation Characteristics and Driving Factors of Tonle Sap Lake’s Surface Water Temperature from 2001 to 2018

Analysis of Variation Characteristics and Driving Factors of Tonle Sap Lake’s Surface Water Temperature from 2001 to 2018

Changes in Microbial Community Structures under Reclaimed Water Replenishment Conditions

Diversity and Activity of Aquatic Cellulolytic Bacteria Isolated from Sedimentary Water in the Littoral Zone of Tonle Sap Lake, Cambodia

Contact Info

Product

Resources

About