Dynamics of bacterial communities before and after distribution in a full-scale drinking water network

El-Chakhtoura, J.; Prest, Emmanuelle I.; Saikaly, Pascal E.; Loosdrecht, M.C.M. van; Hammes, Frederik; Vrouwenvelder, Johannes S.

doi:10.1016/j.watres.2015.02.015

Cited by 116 publications

(70 citation statements)

References 27 publications

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“…In line with other studies (Pinto et al., ; Prest et al., ; Wu et al., ) phyla such as Proteobacteria , Actinobacteria , Firmicutes , Cyanobacteria , Bacteroidetes , and Nitrospirae were commonly found in the water samples investigated. As also observed in this study, several studies have identified Proteobacteria as the most abundant phylum in aquatic environments within the drinking water production industry (Bautista‐de los Santos et al., ; El‐Chakhtoura et al., ; Liu et al., ; Vaz‐Moreira, Nunes, & Manaia, ; Zanacic, McMartin, & Stavrinides, ). It is clear from our results that the bacterial community composition of surface water strongly differs from those of the other water types studied.…”

Section: Discussionsupporting

confidence: 82%

Characterization of the bacterial community composition in water of drinking water production and distribution systems in Flanders, Belgium

et al. 2018

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The quality of drinking water is influenced by its chemical and microbial composition which in turn may be affected by the source water and the different processes applied in drinking water purification systems. In this study, we investigated the bacterial diversity in different water samples from the production and distribution chain of thirteen drinking water production and distribution systems from Flanders (Belgium) that use surface water or groundwater as source water. Water samples were collected over two seasons from the source water, the processed drinking water within the production facility and out of the tap in houses along its distribution network. 454‐pyrosequencing of 16S ribosomal RNA gene sequences revealed a total of 1,570 species‐level bacterial operational taxonomic units. Strong differences in community composition were found between processed drinking water samples originating from companies that use surface water and other that use groundwater as source water. Proteobacteria was the most abundant phylum in all samples. Yet, several phyla including Actinobacteria were significantly more abundant in surface water while Cyanobacteria were more abundant in surface water and processed water originating from surface water. Gallionella , Acinetobacter , and Pseudomonas were the three most abundant genera detected. Members of the Acinetobacter genus were even found at a relative read abundance of up to 47.5% in processed water samples, indicating a general occurrence of Acinetobacter in drinking water (systems).

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Section: Discussionsupporting

confidence: 82%

Characterization of the bacterial community composition in water of drinking water production and distribution systems in Flanders, Belgium

et al. 2018

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“…Similar observations were reported in the literature [23]. However, Proteobacteria are among the most abundant phyla of tap water microbiome in many studies [21][22][23][24].…”

Section: Biodiversity Of Bacteria Forming Biofilm In Cold-and Hot-tapsupporting

confidence: 89%

“…These findings are in contrast with the results of Grabińska-Łoniewska et al [20] but are consistent with the previous study of Wroclaw tap water (data not published). Furthermore, Firmicutes only accounted for around 2% of phyla classification in treatment plant outlet and around 1.1% in a drinking water distribution network in The Netherlands, determined using a pyrosequencing analysis of 16S rRNA gene [21]. Nevertheless, in a huge study of the diversity of microorganisms in drinking water systems of 17 cities in United States, the Bacillus spp.…”

Section: Biodiversity Of Bacteria Forming Biofilm In Cold-and Hot-tapmentioning

confidence: 99%

Application of NGS for studying the antibiotic resistant microbiome in Wrocław tap water

Siedlecka

Piekarska

2018

E3S Web Conf.

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Abstract. The NGS method was used to investigate the biodiversity of ARB dwelling in tap water, able to form biofilm on bullion media supplemented with antibiotics: β-lactam, fluoroquinolone, 3 rd generation cephalosporin and tetracycline. Biofilms formed by cold-and hot-tap water microorganisms were also examined. The presence of 10 ARGs in plasmid DNA of cultivated biomass was tested using a standard PCR. The analyses revealed that the most predominant families were Sphingomonadaceae, Paenibacillaceae, Enterobacteriaceae, Bacillaceae and Sphingomonadaceae in FQ, C3G, T, C-W and H-W samples, respectively (explanations of abbreviations in the text). The ARGs did not correlate with resistance phenotypes.

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“…This number was also approximately one order of magnitude higher than the maximum number of OTUs reported in prior drinking water microbiota surveys (6488 in Lautenschlager et al , 2013; Pinto et al , 2014; El-Chakhtoura et al , 2015; Lührig et al , 2015; Proctor and Hammes, 2015), which have focused exclusively on cold water. We found that elevated water heater temperature drove significant change with respect to both the phylogenetic composition and predicted functions of the microbiota at the tap.…”

Section: Discussionmentioning

confidence: 66%

Impact of water heater temperature setting and water use frequency on the building plumbing microbiome

Rhoads

Edwards

et al. 2017

The ISME Journal

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Hot water plumbing is an important conduit of microbes into the indoor environment and can increase risk of opportunistic pathogens (for example, Legionella pneumophila). We examined the combined effects of water heater temperature (39, 42, 48, 51 and 58 °C), pipe orientation (upward/downward), and water use frequency (21, 3 and 1 flush per week) on the microbial composition at the tap using a pilot-scale pipe rig. 16S rRNA gene amplicon sequencing indicated that bulk water and corresponding biofilm typically had distinct taxonomic compositions (R2Adonis=0.246, PAdonis=0.001), yet similar predicted functions based on PICRUSt analysis (R2Adonis=0.087, PAdonis=0.001). Although a prior study had identified 51 °C under low water use frequency to enrich Legionella at the tap, here we reveal that 51 °C is also a threshold above which there are marked effects of the combined influences of temperature, pipe orientation, and use frequency on taxonomic and functional composition. A positive association was noted between relative abundances of Legionella and mitochondrial DNA of Vermamoeba, a genus of amoebae that can enhance virulence and facilitate replication of some pathogens. This study takes a step towards intentional control of the plumbing microbiome and highlights the importance of microbial ecology in governing pathogen proliferation.

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Dynamics of bacterial communities before and after distribution in a full-scale drinking water network

Cited by 116 publications

References 27 publications

Characterization of the bacterial community composition in water of drinking water production and distribution systems in Flanders, Belgium

Characterization of the bacterial community composition in water of drinking water production and distribution systems in Flanders, Belgium

Application of NGS for studying the antibiotic resistant microbiome in Wrocław tap water

Impact of water heater temperature setting and water use frequency on the building plumbing microbiome

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