Dynamics and reproductive effects of complement factors in the spontaneous abortion model of CBA/J × DBA/2 mice

Takeshita, Ai; Kusakabe, Ken Takeshi; Hiyama, Masato; Kuniyoshi, Nobue; Kondo, Tomohiro; Kanô, Kiyoshi; Kiso, Yasuo; Okada, Toshiya

doi:10.1016/j.imbio.2014.01.001

Cited by 10 publications

(5 citation statements)

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“…In endometrial samples from mouse miscarriages and human placentas, the expression of DAF, MCP, and Crry proteins has been demonstrated 60 61 . Recently, it has been demonstrated in CBA/J×DBA/2 model mice a significant elevation in serum and placenta adipsin levels and that these levels are related to spontaneous abortion 62 . In the present study, the rise of adipsin serum levels in late pregnancy in the preeclamptic group compared to normal pregnant women is observed.…”

Section: Discussionmentioning

confidence: 99%

Serum Adipsin Levels throughout Normal Pregnancy and Preeclampsia

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Garcés

Ruíz-Linares

et al. 2016

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Adipsin is a protease produced at high levels by adipose tissue. It is involved in complement activation and metabolic control. The objective of this study was to determine the changes in adipsin levels during different stages of normal pregnancy, and its association with obstetric outcomes, such as preeclampsia. This nested case-control study in a longitudinal cohort included normal pregnant (n = 54) and preeclamptic (n = 18) women, both followed throughout pregnancy. Additionally, some of the normal pregnant women were followed up three months postpartum (n = 18). Healthy non-pregnant women were also studied during their menstrual cycle (n = 20). The results of this study show that in healthy non-pregnant women, adipsin levels did not change significantly during the menstrual cycle. In normal pregnant women, adipsin levels were lower (p < 0.01) when compared with non-pregnant healthy women, but these serum levels increased again during postpartum (p < 0.001). Adipsin levels were significantly elevated in preeclamptic women in late pregnancy (P < 0.01). A significant correlation was not found between leptin and adipsin during the three periods of gestation studied in healthy pregnant and preeclamptic women. Our results suggest that adipsin may be involved in pregnancy-associated metabolic changes. Moreover, the increase of adipsin levels towards late gestation in preeclamptic women could be related to the pathophysiology of this disease.

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Section: Discussionmentioning

confidence: 99%

Serum Adipsin Levels throughout Normal Pregnancy and Preeclampsia

Poveda

Garcés

Ruíz-Linares

et al. 2016

Sci Rep

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“…Treatment of recombinant adipsin protein affected elevating miscarriage occurrence rates in the early-mid mouse pregnancy period [18]. On the other hand, we simultaneously found the parallel elevation of Crry in the survived implantation sites after adipsin treatment.…”

Section: Introductionmentioning

confidence: 56%

“…Along with adipsin protein, an increase of C3 protein and elevation of complement activity were confirmed in the mouse abortion placenta [ 17 ]. Treatment of recombinant adipsin protein affected elevating miscarriage occurrence rates in the early-mid mouse pregnancy period [ 18 ]. On the other hand, we simultaneously found the parallel elevation of Crry in the survived implantation sites after adipsin treatment.…”

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confidence: 99%

Expression dynamics of Crry at the implantation sites in normal pregnancy and response against miscarriage induction

Kuniyoshi

Hanada

Ando

et al. 2023

The Journal of Veterinary Medical Science

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In mammals, immune tolerance against fetal tissue has been established for normal pregnancy progression. It is known that Crry regulates complemental activity to prevent injury of the mouse embryo and extra-embryonic tissue. This study aimed to examine the expression appearance and normal localization sites of Crry in the mouse placenta. Also, the emergency responses of Crry were verified at the time of experimental miscarriage induction. Moreover, we investigated an existing similar protein of Crry in animal placentas other than mice. Crry expression level showed a peak at day 8.5 of pregnancy.Trophoblast giant cells and decidual cells indicated a positive reaction to anti-Crry antibody. After treatments of interferon-γ, Crry expression was increased significantly in the survived implantation sites as compared with the controls. However, there was no significant difference in the miscarriage-initiated sites. It disclosed that Crry distributes from the early to middle periods of the placentas and involves complement regulation at the extraembryonic tissue and decidua basalis. Crry also showed an ability to respond to risk against external initiation for urgent miscarriage. Finally, we found anti-mouse Crry antibody-bound proteins in the placenta of many animals. It suggests a potency of Crry to make an environment of immune tolerance in many types of mammal placentas.

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“…Our data showed that serum adipsin levels were positively correlated with serum vitamin D, glucagon, and various adipokines such as leptin, resistin, and visfatin. However, other studies suggested a negative role for adipsin in pregnancy being associated with metabolic changes during pregnancy (41,42), including pathogenesis of preeclampsia due to its role in activation of factor B with a direct association with the development of preeclampsia (43,44). Several studies have indicated the important physiological role of adipocytokines in metabolism (45).…”

Section: Discussionmentioning

confidence: 99%

Elevated Adipsin and Reduced C5a Levels in the Maternal Serum and Follicular Fluid During Implantation Are Associated With Successful Pregnancy in Obese Women

Ramanjaneya

Diboun²,

Rizwana

et al. 2022

Front. Endocrinol.

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IntroductionComplement factors mediate the recruitment and activation of immune cells and are associated with metabolic changes during pregnancy. The aim of this study was to determine whether complement factors in the maternal serum and follicular fluid (FF) are associated with in vitro fertilization (IVF) outcomes in overweight/obese women.MethodsForty overweight/obese (BMI = 30.8 ± 5.2 kg/m2) female patients, 33.6 ± 6.3 years old, undergoing IVF treatment for unexplained infertility were recruited. Baseline demographic information, including biochemical hormonal, metabolic, and inflammatory markers, and pregnancy outcome, was collected. Levels of 14 complement markers (C2, C4b, C5, C5a, C9, adipsin, mannose-binding lectin, C1q, C3, C3b/iC3b, C4, factor B, factor H, and properdin) were assessed in the serum and FF and compared to IVF outcome, inflammatory, and metabolic markers using multivariate and univariate models.ResultsOut of 40 IVF cycles, 14 (35%) resulted in pregnancy. Compared to women with failed pregnancies, women with successful pregnancies had higher levels of adipsin in the serum and FF (p = 0.01) but lower C5a levels (p = 0.05). Serum adipsin levels were positively correlated with circulating levels of vitamin D (R = 0.5, p = 0.02), glucagon (R = 0.4, p = 0.03), leptin (R = 0.4, p = 0.01), resistin (R = 0.4, p = 0.02), and visfatin (R = 0.4, p = 0.02), but negatively correlated with total protein (R = −0.5, p = 0.03). Higher numbers of top-quality embryos were associated with increased levels of C3, properdin, C1q, factors H and B, C4, and adipsin, but with reduced C2 and C5a levels (p ≤ 0.01).ConclusionsHigher adipsin and lower C5a levels in the maternal serum during implantation are potential markers of successful outcome in obese women undergoing IVF-assisted pregnancies.

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Dynamics and reproductive effects of complement factors in the spontaneous abortion model of CBA/J × DBA/2 mice

Cited by 10 publications

References 37 publications

Serum Adipsin Levels throughout Normal Pregnancy and Preeclampsia

Serum Adipsin Levels throughout Normal Pregnancy and Preeclampsia

Expression dynamics of Crry at the implantation sites in normal pregnancy and response against miscarriage induction

Elevated Adipsin and Reduced C5a Levels in the Maternal Serum and Follicular Fluid During Implantation Are Associated With Successful Pregnancy in Obese Women

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