Dynamics and diversity of bacteria associated with the disease vectors Aedes aegypti and Aedes albopictus

Bennett, Kelly L.; Martínez, Carmelo Gómez; Chin, Yamileth; Saltonstall, Kristin; McMillan, W. Owen; Rovira, Jose; Loaiza, José R.

doi:10.1038/s41598-019-48414-8

Cited by 47 publications

(50 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, Wolbachia were found at a low abundance and frequency in Florida, USA through 16S rRNA sequencing, and then characterized with multilocus sequence typing (MLST). Bennett et al (2019) and Hegde et al (2018) also detected Wolbachia at a low frequency and abundance through 16S rRNA sequencing but these results could not be validated with PCR amplification. These observations may reflect true infections although there are several potential sources of contamination that can cause false positives (discussed in Chrostek and Gerth (2019)).…”

Section: Limitations Of Studies To Datementioning

confidence: 86%

An elusive endosymbiont: DoesWolbachiaoccur naturally inAedes aegypti?

Ross

Callahan

Yang

et al. 2019

Preprint

View full text Add to dashboard Cite

Wolbachia are maternally-inherited endosymbiotic bacteria found within many insect species. Aedes mosquitoes experimentally infected with Wolbachia are being released into the field for Aedes-borne disease control. These Wolbachia infections induce cytoplasmic incompatibility which is used to suppress populations through incompatible matings or replace populations through the reproductive advantage provided by this mechanism. However the presence of naturally-occurring Wolbachia in target populations could interfere with both population replacement and suppression programs depending on the compatibility patterns between strains. Aedes aegypti were thought to not harbor Wolbachia naturally but several recent studies have detected Wolbachia in natural populations of this mosquito. We therefore review the evidence for natural Wolbachia infections in Ae. aegypti to date and discuss limitations of these studies. We draw on research from other mosquito species to outline the potential implications of natural Wolbachia infections in Ae. aegypti for disease control. To validate previous reports, we obtained a laboratory population of Ae. aegypti from New Mexico, USA, that harbors a natural Wolbachia infection, and we conducted field surveys in Kuala Lumpur, Malaysia where a natural Wolbachia infection has also been reported. However, we were unable to detect Wolbachia infection in both the laboratory and field populations. Because the presence of naturally-occurring Wolbachia in Ae. aegypti could have profound implications for Wolbachia-based disease control programs, it is important to continue to accurately assess the Wolbachia status of target Aedes populations.

show abstract

Section: Limitations Of Studies To Datementioning

confidence: 86%

An elusive endosymbiont: DoesWolbachiaoccur naturally inAedes aegypti?

Ross

Callahan

Yang

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…In this study, Wolbachia were found at a low abundance and frequency in Florida, USA, through 16S rRNA sequencing and then characterized with multilocus sequence typing (MLST). Bennett et al () and Hegde et al () also detected Wolbachia at a low frequency and abundance through 16S rRNA sequencing but these results could not be validated with PCR amplification. These observations may reflect true infections although there are several potential sources of contamination that can cause false positives (discussed in Chrostek and Gerth ()).…”

Section: Limitations Of Studies To Datementioning

confidence: 97%

An elusive endosymbiont: Does Wolbachia occur naturally in Aedes aegypti?

Ross

Callahan

Yang

et al. 2020

Ecology and Evolution

View full text Add to dashboard Cite

Wolbachia are maternally inherited endosymbiotic bacteria found within many insect species. Aedes mosquitoes experimentally infected with Wolbachia are being released into the field for Aedes‐borne disease control. These Wolbachia infections induce cytoplasmic incompatibility which is used to suppress populations through incompatible matings or replace populations through the reproductive advantage provided by this mechanism. However, the presence of naturally occurring Wolbachia in target populations could interfere with both population replacement and suppression programs depending on the compatibility patterns between strains. Aedes aegypti were thought to not harbor Wolbachia naturally but several recent studies have detected Wolbachia in natural populations of this mosquito. We therefore review the evidence for natural Wolbachia infections in A. aegypti to date and discuss limitations of these studies. We draw on research from other mosquito species to outline the potential implications of natural Wolbachia infections in A. aegypti for disease control. To validate previous reports, we obtained a laboratory population of A. aegypti from New Mexico, USA, that harbors a natural Wolbachia infection, and we conducted field surveys in Kuala Lumpur, Malaysia, where a natural Wolbachia infection has also been reported. However, we were unable to detect Wolbachia in both the laboratory and field populations. Because the presence of naturally occurring Wolbachia in A. aegypti could have profound implications for Wolbachia‐based disease control programs, it is important to continue to accurately assess the Wolbachia status of target Aedes populations.

show abstract

“…These two bacterial genera have already been reported in some mosquito species, including Ae. albopictus [20][21][22][23]. Sphingomonas is widely distributed in the environment thanks to its ability to metabolize a wide variety of carbon sources and to survive with few nutrients [24].…”

Section: Taxonomic Composition Of Bacterial and Fungal Communities Inmentioning

confidence: 99%

Comparative Analysis of the Bacterial and Fungal Communities in the Gut and the Crop of Aedes albopictus Mosquitoes: A Preliminary Study

2020

View full text Add to dashboard Cite

The Asian tiger mosquito Aedes albopictus is a major pathogen vector and one of the world’s most invasive species. In recent years, the study of mosquito-associated microbiota has received growing interest for reducing transmission of mosquito-borne pathogens. Most of studies on mosquito microbiota mainly focused on the gut bacteria. However, microorganisms can also colonize other organs and are not restricted to bacteria. In mosquitoes, the crop is the primary storage organ for sugars from the nectar feeding before it is transferred into the midgut for digestion. No study has yet investigated whether this organ can harbor microorganisms in Ae. albopictus. By using high-throughput sequencing, this study is the first to describe the microbiota including both bacteria and fungi in sugar-fed Ae. albopictus males and females. The results showed the presence of diverse and rich bacterial and fungal communities in the crop of both sexes that did not strongly differ from the community composition and structure found in the gut. Altogether, our results provide a thorough description of the crop-associated microbiota in Ae. albopictus which can open new avenues for further studies on trophic interactions between the mosquito and its microbiota.

show abstract

Dynamics and diversity of bacteria associated with the disease vectors Aedes aegypti and Aedes albopictus

Cited by 47 publications

References 76 publications

An elusive endosymbiont: DoesWolbachiaoccur naturally inAedes aegypti?

An elusive endosymbiont: DoesWolbachiaoccur naturally inAedes aegypti?

An elusive endosymbiont: Does Wolbachia occur naturally in Aedes aegypti?

Comparative Analysis of the Bacterial and Fungal Communities in the Gut and the Crop of Aedes albopictus Mosquitoes: A Preliminary Study

Contact Info

Product

Resources

About