Dynamics and Distribution of the Invasive Mosquito Aedes koreicus in a Temperate European City

Kurucz, Kornélia; Manica, Mattia; Delucchi, L.; Kemenesi, Gábor; Marini, Giancarlo

doi:10.3390/ijerph17082728

Cited by 15 publications

(11 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since this Ae . koreicus population seems to have been established in a short period but is restricted to the city of Pécs [ 23 ], the economic center of the Southern Transdanubia region, the high observed haplotype diversity could correlate to the busy international transport of goods through the city. The other Hungarian population at Budapest was represented with a limited sample size in our analyses, but unambiguously conected to the Italian node.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Aedes koreicus, a vector on the rise: Pan-European genetic patterns, mitochondrial and draft genome sequencing

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background The mosquito Aedes koreicus (Edwards, 1917) is a recent invader on the European continent that was introduced to several new places since its first detection in 2008. Compared to other exotic Aedes mosquitoes with public health significance that invaded Europe during the last decades, this species’ biology, behavior, and dispersal patterns were poorly investigated to date. Methodology/Principal findings To understand the species’ population relationships and dispersal patterns within Europe, a fragment of the cytochrome oxidase I (COI or COX1) gene was sequenced from 130 mosquitoes, collected from five countries where the species has been introduced and/or established. Oxford Nanopore and Illumina sequencing techniques were combined to generate the first complete nuclear and mitochondrial genomic sequences of Ae. koreicus from the European region. The complete genome of Ae. koreicus is 879 Mb. COI haplotype analyses identified five major groups (altogether 31 different haplotypes) and revealed a large-scale dispersal pattern between European Ae. koreicus populations. Continuous admixture of populations from Belgium, Italy, and Hungary was highlighted, additionally, haplotype diversity and clustering indicate a separation of German sequences from other populations, pointing to an independent introduction of Ae. koreicus to Europe. Finally, a genetic expansion signal was identified, suggesting the species might be present in more locations than currently detected. Conclusions/Significance Our results highlight the importance of genetic research of invasive mosquitoes to understand general dispersal patterns, reveal main dispersal routes and form the baseline of future mitigation actions. The first complete genomic sequence also provides a significant leap in the general understanding of this species, opening the possibility for future genome-related studies, such as the detection of ‘Single Nucleotide Polymorphism’ markers. Considering its public health importance, it is crucial to further investigate the species’ population genetic dynamic, including a larger sampling and additional genomic markers.

show abstract

Section: Resultsmentioning

confidence: 99%

“…At the same time, Ae . koreicus was found in Baranya county, southwest Hungary [ 22 ], where an overwintering but localized population has established [ 23 ], but a few years later (2019), it appeared in the capital city of Budapest, north-central Hungary [ 24 ]. Recently, Ae .…”

Section: Introductionmentioning

confidence: 99%

Aedes koreicus, a vector on the rise: Pan-European genetic patterns, mitochondrial and draft genome sequencing

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The way of introduction could not be identified with certainty, as no link could be made with an international trade route (Versteirt et al, 2012). Besides Belgium, introduction and/or establishment of this species in Europe has been described from Germany (Pfitzner et al, 2018;Steinbrink et al, 2019;Werner et al, 2016), Hungary (Kurucz et al, 2016(Kurucz et al, , 2020, Italy (Ballardini et al, 2019;Gradoni et al, 2021;Marcantonio et al, 2016;Suter et al, 2015), Slovenia (Kalan et al, 2017), Switzerland (Cherix, 2019), Russia (Bezzhonova et al, 2014) and Ukraine (Kovalenko and Tikhonov, 2019) in accordance with the ECDC vector map (ECDC, 2021). Whereas for most Aedes IMS several pathways of introduction have been identified with certainty, no pathways have been confirmed for Ae.…”

Section: Discussionmentioning

confidence: 99%

First finding of Aedes koreicus (Diptera: Culicidae) in the Netherlands

Teekema

Stroo

Uiterwijk

et al. 2022

JEMC

View full text Add to dashboard Cite

Since 2010, the Centre for Monitoring of Vectors (CMV) of the Netherlands Food and Consumer Product Safety Authority (NVWA), has a surveillance programme in place to detect and control invasive mosquito species (IMS) at locations with increased risk of importation. At the premises of companies that import used tyres from risk areas, the CMV implements surveillance using adult mosquito traps. In case of an IMS finding at a used tyre company location, the monitoring is intensified here (weekly sampling, additional BG-Sentinel traps and larval sampling), as well as within a predefined area with a radius of 500 m from the limits of the used tyre company location. On September 6th, 2021, eight Aedes larvae were found in a sample taken from a water-containing bucket. These larvae were both morphologically and molecularly (Illumina sequencing) identified as Aedes koreicus. Additional sampling at this first finding site of Ae. koreicus in the Netherlands, which was followed by mosquito control using larvicides, did not lead to further findings of the species.

show abstract

“…japonicus , which generally exhibits a considerable active and passive dispersal rate in Europe, although stationary populations have been observed [ 24 , 25 ], European populations of Ae. koreicus have mainly remained quite stationary and restricted to the area of initial establishment [ 44 , 45 ]. Thus, the observed negligible spread of the German population fits well with studies on other European Ae.…”

Section: Discussionmentioning

confidence: 99%

The invasive Korean bush mosquito Aedes koreicus (Diptera: Culicidae) in Germany as of 2020

2021

View full text Add to dashboard Cite

Background The Korean bush mosquito Aedes koreicus was recently reported to have established a population in western Germany (Wiesbaden) in 2016. The species is difficult to distinguish morphologically from its close relative, the invasive Japanese bush mosquito Ae. japonicus, which is already widely distributed in many parts of Germany, including the area colonised by Ae. koreicus. Genetic confirmation of morphologically identified “Ae. japonicus” collection material, however, had only been done exceptionally before the German Ae. koreicus population became known. Methods Dried archived “Ae. japonicus” specimens both from the municipality of Wiesbaden and from deliberately and randomly selected distribution sites all over Germany were re-examined morphologically and genetically for admixture by Ae. koreicus. Moreover, cemeteries in the greater Wiesbaden area were sampled in 2019 and 2020 to check for Ae. koreicus spread. Korean and Japanese bush mosquitoes submitted to the German citizen science mosquito monitoring scheme “Mueckenatlas” in 2019 and 2020 were also subjected to particularly thorough species identification. The ND4 DNA sequences generated in this study in the context of species identification were phylogenetically compared to respective GenBank entries of Ae. koreicus. As a by-product, several genetic markers were evaluated for their suitability to identify Ae. koreicus. Results Aedes koreicus specimens could be identified in mosquito collection material and submissions from Wiesbaden from 2015 onwards, suggesting establishment to have happened in the same year as Ae. japonicus establishment. Detections of Ae. koreicus from 2019 and 2020 in Wiesbaden indicate a negligible enlargement of the populated area as described for 2018. Two Ae. koreicus specimens were also submitted from the city of Munich, southern Germany, in 2019 but further specimens could not be identified during immediate local inspections. Comparison of ND4 sequences generated in this and other studies demonstrate a high degree of homology, suggesting that this DNA region is not informative enough for clarification of origins and relationships of Ae. koreicus populations. For genetic identification of Ae. koreicus, PCR primers used for classical CO1 barcoding were found to lead to mismatches and produce no or incorrect amplicons. Alternative CO1 primers or a validated ND4 marker should be used instead. Conclusions Aedes koreicus is probably introduced into Germany every now and then but rarely succeeds in becoming established. As with most European populations, the German population is characterised by a limited expansion tendency. Since Ae. koreicus is a potential vector, however, Asian bush mosquitoes found at new places should be examined quite carefully and known distribution areas of Ae. japonicus regularly checked for the presence of Ae. koreicus. Graphical Abstract

show abstract

Dynamics and Distribution of the Invasive Mosquito Aedes koreicus in a Temperate European City

Cited by 15 publications

References 33 publications

Aedes koreicus, a vector on the rise: Pan-European genetic patterns, mitochondrial and draft genome sequencing

Aedes koreicus, a vector on the rise: Pan-European genetic patterns, mitochondrial and draft genome sequencing

First finding of Aedes koreicus (Diptera: Culicidae) in the Netherlands

The invasive Korean bush mosquito Aedes koreicus (Diptera: Culicidae) in Germany as of 2020

Contact Info

Product

Resources

About