Dynamically resizable nanometre-scale apertures for molecular sensing

Sowerby, Stephen J.; Broom, Murray F.; Petersen, George B.

doi:10.1016/j.snb.2006.08.031

Cited by 95 publications

(103 citation statements)

References 14 publications

(19 reference statements)

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“…A relatively recent technology to be developed for the characterisation of nanoparticles is based upon tunable resistive pulse sensing (TRPS) [44][45][46][47][48][49][50][51] . TRPS is based on polyurethane elastomeric membranes in which the pore geometry can be altered in real time.…”

Section: Introductionmentioning

confidence: 99%

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

2016

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Resistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterised as they traverse pores. Previous work using RPS has shown that the size, concentration and zeta potential of the analyte can be measured. Here we use tunable resistive pulse sensing (TRPS) which utilises a tunable pore to monitor the translocation times of nanoparticles with DNA modified surfaces. We start by demonstrating that the translocation times of particles can be used to infer the zeta potential of known standards and then apply the method to measure the change in zeta potential of DNA modified particles. By measuring the translocation times of DNA modified nanoparticles as a function of packing density, length, structure, and hybridisation time, we observe a clear difference in zeta potential using both mean values, and population distributions as a function of the DNA structure. We demonstrate the ability to resolve the signals for ssDNA, dsDNA, small changes in base length for nucleotides between 15-40 bases long and even the discrimination between partial and fully complementary target sequences. Such a method has potential and applications in sensors for the monitoring of nanoparticles in both medical and environmental samples.

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Section: Introductionmentioning

confidence: 99%

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

2016

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“…The sophistication of resistive pulse sensing has also increased, making use of channels made from carbon nanotubes, 17,18 glass, [19][20][21][22][23] silicon, 5,8,15,24 polymers, [25][26][27][28] and elastomers. [29][30][31][32][33][34][35][36][37][38] Resistive pulses can now be used for study and measurement of particle size, 2,3,17,18,20,21,34 concentration, 33 and charge. 17,38 There is clear potential to extend applications towards sensing of complicated dynamic interactions 24 and specific particle shapes.…”

mentioning

confidence: 99%

“…TPs are fabricated by puncturing a thin elastomeric membrane. 29,30 This membrane can be reversibly actuated on macroscopic scales in order to stretch and relax the micro-to nanoscale conical pore geometry. 31 The ionic current passing through a TP can vary by at least an order of magnitude when the pore is stretched.…”

mentioning

confidence: 99%

“…TPs can be manufactured to target any particular particle type(s) of the order of tens of nanometers or larger, and sensing of individual DNA plasmids has been reported. 29 In addition to particle size, 34 concentration, 33 and charge, 38 TPs have been used to study multimodal particle size distributions, 30,36 surface-modified particles, 36,37 and the effects of solution pH on particle transport. 33 Recently, a semi-analytic approach was developed to simulate resistive pulses in TPs, and this was used to investigate pulse asymmetry.…”

mentioning

confidence: 99%

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Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

Willmott

Platt

2012

Biomicrofluidics

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Tunable pores (TPs) have been used for resistive pulse sensing of 1 lm superparamagnetic beads, both dispersed and within a magnetic field. Upon application of this field, magnetic supraparticle structures (SPSs) were observed. Onset of aggregation was most effectively indicated by an increase in the mean event magnitude, with data collected using an automated thresholding method. Simulations enabled discrimination between resistive pulses caused by dimers and individual particles. Distinct but time-correlated peaks were often observed, suggesting that SPSs became separated in pressure-driven flow focused at the pore constriction. The distinct properties of magnetophoretic and pressure-driven transport mechanisms can explain variations in the event rate when particles move through an asymmetric pore in either direction, with or without a magnetic field applied. Use of TPs for resistive pulse sensing holds potential for efficient, versatile analysis and measurement of nano-and microparticles, while magnetic beads and particle aggregation play important roles in many prospective biosensing applications.

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“…Tunable pore membranes are made of thermoplastic polyurethane and the pores are introduced by mechanical puncture. The concept of tunable nanopore membrane was first developed by Sowerby [20]. The membrane can be stretched in a biaxial direction to alter the pore size.…”

Section: Tunable Resistive Pulse Sensing (Trps)mentioning

confidence: 99%

Tunable Resistive Pulse Sensing: Potential Applications in Nanomedicine

Sivakumaran

Platt

2016

Nanomedicine (Lond.)

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An accurate characterisation of nanomaterials used in clinical diagnosis and therapeutics is of paramount importance to realise the full potential of nanotechnology in medicine and to avoid unexpected and potentially harmful toxic effects due to these materials. A number of technical modalities are currently in use to study the physical, chemical and biological properties of nanomaterials but they all have advantages and disadvantages. In this review, we discuss the potential of a relative newcomer, Tunable Resistive Pulse Sensing (TRPS), for the characterisation of nanomaterials and its applications in nanodiagnostics.

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Dynamically resizable nanometre-scale apertures for molecular sensing

Cited by 95 publications

References 14 publications

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

Tunable Resistive Pulse Sensing: Potential Applications in Nanomedicine

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