Dynamically Crosslinked Gold Nanoparticle – Hyaluronan Hydrogels

Skardal, Aleksander; Zhang, Jianxing; McCoard, Lindsi; Oottamasathien, Siam; Prestwich, Glenn D.

doi:10.1002/adma.201001436

Cited by 219 publications

(157 citation statements)

References 19 publications

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“…1 Partial pre-crosslinking of the hydrogel precursors is an attractive approach to facilitate a good shape fidelity of printed constructs using low polymer concentrations. [23][24][25] Rutz et al 24 described partial precrosslinking of functionalized poly(ethylene glycol) (PEG) cross-linkers with natural polymers, thereby forming a variety of 3D-printed hydrogels with low polymer contents. These bioinks were deposited with a low flow, without collapse and resulting constructs maintained high shape fidelity.…”

Section: Introductionmentioning

confidence: 99%

Biofabrication of reinforced 3D-scaffolds using two-component hydrogels

et al. 2015

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Biofabrication of reinforced 3D-scaff olds using two-component hydrogels Two strategies were developed to 3D print two-component hydrogels by exploiting polymers with thermogelling and chemoselective crosslinking properties. Pre-crosslinking by oxo-ester mediated native chemical ligation of the two components results in well-defi ned 3D printed constructs. Alternatively, mechanical reinforcement of these hydrogels through covalent cross-linking with a functionalized thermoplastic polymer can be achieved by the same chemoselective chemistry. Good cell compatibility was observed for hyaluronic acid containing scaff olds indicating their potential as new materials for biofabrication applications. precursors proceeded after extrusion to form mechanically stable hydrogels that exhibited a storage modulus of 9 kPa after 3 hours. Flow and elastic properties of the polymer solutions and hydrogels were analyzed under similar conditions to those used during the 3D-printing process. These experiments showed the ability to extrude the hydrogels, as well as their rapid recovery after applied shear forces. Hydrogels were printed in grid-like structures, hollow cones and a model representing a femoral condyle, with a porosity of 48 AE 2%. Furthermore, an N-hydroxysuccinimide functionalized thermoplastic poly-e-caprolactone (PCL) derivative was successfully synthesized and 3D-printed. We demonstrated that covalent grafting of the developed hydrogel to the thermoplastic reinforced network resulted in improved mechanical properties and yielded high construct integrity. Reinforced constructs also containing hyaluronic acid showed high cell viability of chondrocytes, underlining their potential for further use in regenerative medicine applications.

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Section: Introductionmentioning

confidence: 99%

Biofabrication of reinforced 3D-scaffolds using two-component hydrogels

et al. 2015

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“…Skardal et al introduced gold nanoparticles (AuNPs) into semi-synthetic extracellular matrix (sECM) hydrogel composites which can generate dynamic crosslinking between intra-gel and inter-gel during and after printing. In particular, 2.5% w/ v of 25 nm gold nanoparticle provided enough mechanical stability to support multilayered 3D structures by the physical reinforcement effect, and after 60 min of printing, adjacent filaments were completely joined by slow rate of inter-filament crosslinking between AuNP and sECM hydrogel [86] . This dynamically crosslinked AuNP-sECM hydrogel may provide new strategies in the ex situ particle incorporation approach for the 3D printing hydrogel composite system.…”

Section: Particle-reinforced Hydrogel Composites 3d Printingmentioning

confidence: 99%

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

Jang¹,

Jung²,

Pan³

et al. 2024

IJB

188

117

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Three-dimensional (3D) printing of hydrogels is now an attractive area of research due to its capability to fabricate intricate, complex and highly customizable scaffold structures that can support cell adhesion and promote cell infiltration for tissue engineering. However, pure hydrogels alone lack the necessary mechanical stability and are too easily degraded to be used as printing ink. To overcome this problem, significant progress has been made in the 3D printing of hydrogel composites with improved mechanical performance and biofunctionality. Herein, we provide a brief overview of existing hydrogel composite 3D printing techniques including laser based-3D printing, nozzle based-3D printing, and inkjet printer based-3D printing systems. Based on the type of additives, we will discuss four main hydrogel composite systems in this review: polymer-or hydrogel-hydrogel composites, particle-reinforced hydrogel composites, fiber-reinforced hydrogel composites, and anisotropic filler-reinforced hydrogel composites. Additionally, several emerging potential applications of hydrogel composites in the field of tissue engineering and their accompanying challenges are discussed in parallel.

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“…HA forms very soft gels but can be modified and crosslinked using a variety of methods including the UV method described in Section 4.1 [76] and thiol-modified HA using gold nanoparticles [77] to increase its stiffness. Similarly, fibrin is already used in surgery as a haemostatic agent and sealant [78,79] .…”

Section: Natural Materialsmentioning

confidence: 99%

3D bioprinting for tissue engineering: Stem cells in hydrogels

Mehrban¹,

Teoh²,

Birchall³

2024

IJB

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Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in patients. However, the demand for such tissues greatly outweighs the supply and a fast and accurate method of production is still required. 3D bioprinting offers precision control as well as the ability to incorporate biological cues and cells directly into the material as it is being fabricated. Having precise control over scaffold morphology and chemistry is a significant step towards controlling cellular behaviour, particularly where undifferentiated cells, i.e., stem cells, are used. This level of control in the early stages of tissue development is crucial in building more complex systems that morphologically and functionally mimic in vivo tissue. Here we review 3D printing hydrogel materials for tissue engineering purposes and the incorporation of cells within them. Hydrogels are ideal materials for cell culture. They are structurally similar to native extracellular matrix, have a high nutrient retention capacity, allow cells to migrate and can be formed under mild conditions. The techniques used to produce these materials, as well as their benefits and limitations, are outlined.

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Dynamically Crosslinked Gold Nanoparticle – Hyaluronan Hydrogels

Cited by 219 publications

References 19 publications

Biofabrication of reinforced 3D-scaffolds using two-component hydrogels

Biofabrication of reinforced 3D-scaffolds using two-component hydrogels

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

3D bioprinting for tissue engineering: Stem cells in hydrogels

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