Dynamical properties of LFPs from mice with unilateral injection of TeNT

Vannini, Eleonora; Caleo, Matteo; Chillemi, Santi; Garbo, Angelo Di

doi:10.1016/j.biosystems.2017.09.009

Cited by 7 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Paracetamol was added in drinking water for 3 days. Additional details can be found in Mainardi et al, 2012; Vallone et al, 2016; Vannini et al, 2016, 2017. No behavioural seizures are detectable in those animals, as already reported in (Mainardi et al, 2012; Vannini et al, 2016).…”

Section: Methodssupporting

confidence: 67%

See 1 more Smart Citation

Synaptic vesicles dynamics in neocortical epilepsy

Vannini

Restani

Dilillo

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Neuronal networks’ hyperexcitability often results from an unbalance between excitatory and inhibitory neurotransmission; however, underlying synaptic alterations leading to this condition remains poorly understood. Here, we assess synaptic changes in the visual cortex of epileptic tetanus neurotoxin-injected mice. Using an ultrastructural measure of synaptic activity, we quantified functional differences at excitatory and inhibitory synapses. We found homeostatic changes in hyperexcitable networks, expressed as an early onset lengthening of active zones at inhibitory synapses followed by spatial reorganization of recycled vesicles at excitatory synapses. A proteomic analysis of synaptic content revealed an upregulation of Carboxypeptidase E (CPE) following Tetanus NeuroToxin (TeNT) injection. Remarkably, inhibition of CPE rapidly decreased network discharges in vivo. These analyses reveal a complex landscape of homeostatic changes affecting the epileptic synaptic release machinery, differentially at inhibitory and excitatory terminals. Our study unveil homeostatic presynaptic mechanisms which may impact release timing rather than synaptic strength.

show abstract

Section: Methodssupporting

confidence: 67%

“…All experimental procedures were conducted in conformity with the European Communities Council Directive n• 86/609/EEC and were approved by the Italian Ministry of Health. TeNT injections were performed as previously described (Mainardi et al, 2012;Vallone et al, 2016;Vannini et al, , 2017.…”

Section: Animals and Tent Injectionsmentioning

confidence: 99%

Synaptic vesicles dynamics in neocortical epilepsy

Vannini

Restani

Dilillo

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Paracetamol was added in drinking water for 3 days. Additional details can be found in Mainardi et al (2012); Vallone et al (2016), and Vannini et al ( , 2017. No behavioral seizures are detectable in those animals, as already reported in Mainardi et al (2012) and Vannini et al (2016).…”

Section: Animals and Tent Injectionssupporting

confidence: 66%

Synaptic Vesicles Dynamics in Neocortical Epilepsy

Vannini

Restani

Dilillo

et al. 2020

Front. Cell. Neurosci.

Self Cite

View full text Add to dashboard Cite

Neuronal hyperexcitability often results from an unbalance between excitatory and inhibitory neurotransmission, but the synaptic alterations leading to enhanced seizure propensity are only partly understood. Taking advantage of a mouse model of neocortical epilepsy, we used a combination of photoconversion and electron microscopy to assess changes in synaptic vesicles pools in vivo. Our analyses reveal that epileptic networks show an early onset lengthening of active zones at inhibitory synapses, together with a delayed spatial reorganization of recycled vesicles at excitatory synapses. Proteomics of synaptic content indicate that specific proteins were increased in epileptic mice. Altogether, our data reveal a complex landscape of nanoscale changes affecting the epileptic synaptic release machinery. In particular, our findings show that an altered positioning of release-competent vesicles represent a novel signature of epileptic networks.

show abstract

“…Tetanospasmin also can generate paralysis by stopping transmission at the neuromuscular junctions 9) . Some clinical manifestations indicate the involvement of the sympathetic nervous system.…”

Section: Main Subject 1 Bacteriology and Pathogenesismentioning

confidence: 99%

Neonatal tetanus

Brook

2021

Pediatr Emerg Med J

View full text Add to dashboard Cite

Neonatal tetanus, also known as tetanus neonatorum, occurs in young infants of inadequately immunized mothers. It is a kind of generalized tetanus that is exhibited mainly by prevention of the release of the inhibitory neurotransmitters (i.e., disinhibition) and is initiated by tetanospasmin, an exotoxin created by Clostridium tetani. Contamination of the umbilical cord stump is the main cause. The typical, early manifestations are weakness and lack of ability to suck, continuing within hours to trismus, risus sardonicus, and ultimately generalized tetanic spasm, rigidity, and opisthotonus. Without treatment, neonatal tetanus has a poor outcome with a mortality rate above 90%. Mortality can result from asphyxia due to the spasm and hypersympathetic state. The managing goals are to neutralize its toxin, eradicate C. tetani, care for wound, and offer supportive care, such as mechanical ventilation, parenteral nutrition, sedation, neuromuscular blockade, and management of autonomic dysfunction.

show abstract

Dynamical properties of LFPs from mice with unilateral injection of TeNT

Cited by 7 publications

References 35 publications

Synaptic vesicles dynamics in neocortical epilepsy

Synaptic vesicles dynamics in neocortical epilepsy

Synaptic Vesicles Dynamics in Neocortical Epilepsy

Neonatal tetanus

Contact Info

Product

Resources

About