Dynamic viscoelastic properties of collagen gels with high mechanical strength

Mori, Hideki; Shimizu, Kousuke; Hara, Masayuki

doi:10.1016/j.msec.2013.03.047

Cited by 35 publications

(15 citation statements)

References 25 publications

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“…In general, the addition of chains to an amphiphilic copolymer causes changes in sol-gel transition behavior of the conjugated chains [35,36]. From the results, the grafted heparin in the HP structure increased the critical gelation concentration (CGC) of poloxamer 407.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of a Novel Thermosensitive Heparin-Poloxamer Hydrogel for Improving Vascular Anastomosis Quality and Safety in a Rabbit Model

Zhao

et al. 2013

PLoS ONE

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Despite progress in the design of advanced surgical techniques, stenosis recurs in a large percentage of vascular anastomosis. In this study, a novel heparin-poloxamer (HP) hydrogel was designed and its effects for improving the quality and safety of vascular anastomosis were studied. HP copolymer was synthesized and its structure was confirmed by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (1H-NMR). Hydrogels containing HP were prepared and their important characteristics related to the application in vascular anastomosis including gelation temperature, rheological behaviour and micromorphology were measured. Vascular anastomosis were performed on the right common carotid arteries of rabbits, and the in vivo efficiency and safety of HP hydrogel to achieve vascular anastomosis was verified and compared with Poloxamer 407 hydrogel and the conventional hand-sewn method using Doppler ultrasound, CT angiograms, scanning electron microscopy (SEM) and histological technique. Our results showed that HP copolymer displayed special gel-sol-gel phase transition behavior with increasing temperature from 5 to 60 °C. HP hydrogel prepared from 18 wt% HP solution had a porous sponge-like structure, with gelation temperature at approximately 38 °C and maximum elastic modulus at 10,000 Pa. In animal studies, imaging and histological examination of rabbit common jugular artery confirmed that HP hydrogel group had similar equivalent patency, flow and burst strength as Poloxamer 407 group. Moreover, HP hydrogel was superior to poloxamer 407 hydrogel and hand-sewn method for restoring the functions and epithelial structure of the broken vessel junctions after operation. By combining the advantages of heparin and poloxamer 407, HP hydrogel holds high promise for improving vascular anastomosis quality and safety.

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Section: Resultsmentioning

confidence: 99%

Evaluation of a Novel Thermosensitive Heparin-Poloxamer Hydrogel for Improving Vascular Anastomosis Quality and Safety in a Rabbit Model

Zhao

et al. 2013

PLoS ONE

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“…for medical use such as tissue engineering (Smith et al, 2000) and cellular matrices (Nagai et al, 2008). However, previous study showed that the mechanical strength of the cross-linked collagen gels is hardly to support the medical purpose (Mori, Shimizu, & Hara, 2013). But in this study, the optimal gel-forming conditions for SC-gel with higher mechanical strength were obtained.…”

Section: Mechanical Strength Of Gel After Cross-linkingmentioning

confidence: 72%

“…Obviously, after EDC cross‐linking, the mechanical strength of SC‐gel has been improved significantly ( p < .05), which can be cross‐linked by various methods and then mechanically reinforced, for medical use such as tissue engineering (Smith et al, ) and cellular matrices (Nagai et al, ). However, previous study showed that the mechanical strength of the cross‐linked collagen gels is hardly to support the medical purpose (Mori, Shimizu, & Hara, ). But in this study, the optimal gel‐forming conditions for SC‐gel with higher mechanical strength were obtained.…”

Section: Resultsmentioning

confidence: 99%

Characterization of three-spot seahorse (Hippocampus trimaculatus ) skin collagen and its fibrillar gel reinforced by EDC: A comparative study

et al. 2017

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In current study, three‐spot seahorse (Hippocampus trimaculatus) skin collagen (SC) was extracted for the first time, and its physicochemical properties were characterized. Nile tilapia‐skin collagen (TC) and pig‐skin collagen (PC) were used to the references. The yields of SC and TC were 40.41 ± 1.23 and 52.6 ± 0.97% (dry weight), respectively. The SDS‐PAGE and FTIR confirmed that SC, TC, and PC were preliminarily identified as type I collagen with triple helical structure. The denaturation temperatures of SC, TC, and PC were 35.12, 38.66, and 42.56°C, respectively. Gel‐forming studies indicated SC exhibited higher capability than TC and PC under the effect of NaCl. After cross‐linking by 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide (EDC), SC showed a significant increase in thermal stability and mechanical strength, and electron microscopic observation corroborated this phenomenon. Therefore, the isolated collagen from three‐spot seahorse skin can be used in food packaging and biomaterials as alternatives to mammalian collagen. Practical applications In this study, we evaluated a new source of skin collagen from three‐spot seahorse which has been large‐scale artificially cultured in recent years. The finding of this study demonstrate that SC exhibited higher gel‐forming capability under the effect of NaCl, which suggest that it may be used in photographic film, sausage casings, and food additive as alternatives to mammalian collagen. Furthermore, thermal stability and mechanical strength of SC gel have a significant increase after cross‐linking by EDC, so it can be further used for biomaterials, such as food packaging, drug delivery, tissue engineering constructs, and wound dressing systems.

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“…The riboflavin concentration and the irradiation time showed a significant influence on the degree of crosslinking between collagen molecules, [14] which led to the increase of the density, the diameter and the mechanical strength of collagen fibres. [15,16] Through the above crosslinking method, the defects of collagen film and its mechanical properties were improved, which was of great significance in food packaging.…”

Section: Introductionmentioning

confidence: 99%

Combined effects of glutaraldehyde and riboflavin/uv365 on the self-assembly of type I collagen molecules observed with atomic force microscopy

Song

Yang

et al. 2018

International Journal of Food Properties

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The aim of this study was to demonstrate the effect of different treatments on collagen self-assembly, the morphological and the qualitative analysis of collagen assembly were measured by atomic force microscopy. Collagen samples were treated with 0.02%, 0.04%, 0.10% and 0.20% glutaraldehyde individually at the first steps. Meanwhile, the other experimental groups were treated with 0.05%, 0.10%, 0.20% riboflavin, respectively, the structural change of the collagen network with 15, 30, 45, 60 min UV365 exposure and the fluorescent irradiation as the control group in turn was investigated by atomic force microscopy. The width and height of collagen assembly increased with the concentration of glutaraldehyde, which were changed from 36.45 ± 4.45nm to 64.35 ± 2.58nm and 2.26 ± 0.19nm to 4.93 ± 0.29 nm, respectively. The effect of different UV365 irradiation time on the height of collagen was greater than the effect on the width, the width and height of collagen were varied from 41.36 ± 4.30nm to 55.47 ± 4.53nm and 3.10 ± 0.39nm to 6.20 ± 0.85nm, which were changed 25.44% and 50.00%, respectively. The concentration of riboflavin was more efficiently than irradiation time, and the maximum width and height of collagen reached 106.98 ± 3.37nm and 11.17 ± 1.33 nm, respectively, the control group without a significant change at the fluorescent irradiation by the time. This optimized cross-linking method with riboflavin/UV365 would have great potential for tissue engineering in clinic and food packaging science.

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Dynamic viscoelastic properties of collagen gels with high mechanical strength

Cited by 35 publications

References 25 publications

Evaluation of a Novel Thermosensitive Heparin-Poloxamer Hydrogel for Improving Vascular Anastomosis Quality and Safety in a Rabbit Model

Evaluation of a Novel Thermosensitive Heparin-Poloxamer Hydrogel for Improving Vascular Anastomosis Quality and Safety in a Rabbit Model

Characterization of three-spot seahorse (Hippocampus trimaculatus ) skin collagen and its fibrillar gel reinforced by EDC: A comparative study

Combined effects of glutaraldehyde and riboflavin/uv365 on the self-assembly of type I collagen molecules observed with atomic force microscopy

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