Dynamic transcriptomic analysis uncovers key genes and mechanisms involved in seed priming-induced tolerance to drought in barley

Sazegari, Sima; Zinati, Zahra; Tahmasebi, Ahmad

doi:10.1016/j.genrep.2020.100941

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…Currently, many studies indicate that the barley transcription factor is involved in drought tolerance. Sazegari et al [ 22 ] reported that ERF/AP2 , C2C2-Dof , and bHLH transcription factor families are regulatory components of the transcriptional cascade involved in priming-induced tolerance. The expression of the gene for the barley MYB transcription factor, HvMYB1 , was reportedly upregulated in roots and leaves by drought, which reduced stomatal conductance, enhanced proline content, and reduced ROS levels and catalase activity [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

New insights on key genes involved in drought stress response of barley: gene networks reconstruction, hub, and promoter analysis

Javadi

Shobbar

Ebrahimi

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Barley (Hordeum vulgare L.) is one of the most important cereals worldwide. Although this crop is drought-tolerant, water deficiency negatively affects its growth and production. To detect key genes involved in drought tolerance in barley, a reconstruction of the related gene network and discovery of the hub genes would help. Here, drought-responsive genes in barley were collected through analysis of the available microarray datasets (− 5 ≥ Fold change ≥ 5, adjusted p value ≤ 0.05). Protein-protein interaction (PPI) networks were reconstructed. Results The hub genes were identified by Cytoscape software using three Cyto-hubba algorithms (Degree, Closeness, and MNC), leading to the identification of 17 and 16 non-redundant genes at vegetative and reproductive stages, respectively. These genes consist of some transcription factors such as HvVp1, HvERF4, HvFUS3, HvCBF6, DRF1.3, HvNAC6, HvCO5, and HvWRKY42, which belong to AP2, NAC, Zinc-finger, and WRKY families. In addition, the expression pattern of four hub genes was compared between the two studied cultivars, i.e., “Yousef” (drought-tolerant) and “Morocco” (susceptible). The results of real-time PCR revealed that the expression patterns corresponded well with those determined by the microarray. Also, promoter analysis revealed that some TF families, including AP2, NAC, Trihelix, MYB, and one modular (composed of two HD-ZIP TFs), had a binding site in 85% of promoters of the drought-responsive genes and of the hub genes in barley. Conclusions The identified hub genes, especially those from AP2 and NAC families, might be among key TFs that regulate drought-stress response in barley and are suggested as promising candidate genes for further functional analysis.

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Section: Introductionmentioning

confidence: 99%

New insights on key genes involved in drought stress response of barley: gene networks reconstruction, hub, and promoter analysis

Javadi

Shobbar

Ebrahimi

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…The responses of genotypes to drought stress exhibit variability. For instance, the cultivar Sebastian is acknowledged for its drought tolerance [ 50 , 51 , 52 ]. However, when compared to other cultivars, its responsiveness is limited to specific physiological parameters, displaying an average reaction to drought stress [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Endophyte Inoculation and Elevated Potassium Supply on Productivity, Growth and Physiological Parameters of Spring Barley (Hordeum vulgare L.) Genotypes over Contrasting Seasons

Bleša,

Matušinský,

Baláž

et al. 2024

Plants

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In recent years, recurrent droughts have significantly affected spring barley production, reducing the quantity and quality of grain. This study aims to identify genotype-specific traits and the drought resilience of six different Hordeum vulgare L. (spring barley) genotypes, while also examining the potential of potassium application and fungal endophyte Serendipita indica inoculation to mitigate the negative effects of dry periods during the growing season. Field experiments were conducted over a three-year period from 2020 to 2022, measuring physiological, growth, and yield parameters. To get insight into the physiological state of the plants, we measured the soluble sugars content and the ratio of stable carbon isotopes in the flag leaf tissue, which reflects conditions during its formation. The dominant factors that influenced the measured parameters were the genotypes and seasons, as well as their interaction, rather than other experimental factors. The results showed that the Spitfire and Accordine varieties were the best performing in both the 2020 and 2021 seasons, as indicated by their yield. However, in the drier 2022 season, the yield of these two varieties decreased significantly (to 55% for Spitfire and to 69% for Accordine of their yield in 2021), while for the arid-region genotypes, it remained at the same level as the previous year. This study sheds light on the potential of various genotypes to withstand periods of drought and the effectiveness of using potassium application and S. indica inoculation as mitigation approaches.

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“…Among these, photosynthesis occupies a central position. Additionally, protein-chromophore linkage influences protein function and signalling pathways [35]. Ribosomal large subunit biogenesis enhances protein synthesis mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Plant Defence Induction by Meyerozyma guilliermondii in Vitis vinifera L.

Alonso de Robador,

Ortega Pérez,

Sanchez-Ballesta

et al. 2023

Agronomy

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This article emphasizes the crucial importance of yeast Meyerozyma guilliermondii (Patent CECT13190) as a biological control agent (BCA) in eliciting defensive responses in vine plants, and is supported by comprehensive physiological, proteomic, and transcriptomic analyses. The results demonstrate that the BCA M. guilliermondii can induce enhanced defensive responses, as reflected in the regulation of key proteins. Notably, the upregulated expression of calmodulin and pathogenesis-related protein 10 (PR-10) are indicative of a complex interplay between calcium signalling, salicylic acid accumulation, and the elicitation of plant defence responses against pathogens. Furthermore, changes in microtubule dynamics and proteins related to protein synthesis and folding are observed, confirming the elicitation of defence responses. The correspondence between proteomic and transcriptomic analyses for genes codifying pathogenesis-related proteins, such as Vcgns1, VviTL1, and Vcchit1b, reinforces the empirical robustness of our findings. Collectively, our research explores the modulation of plant defences by the BCA, opening promising avenues for innovative agricultural strategies that enhance crop resilience and productivity.

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Dynamic transcriptomic analysis uncovers key genes and mechanisms involved in seed priming-induced tolerance to drought in barley

Cited by 6 publications

References 29 publications

New insights on key genes involved in drought stress response of barley: gene networks reconstruction, hub, and promoter analysis

New insights on key genes involved in drought stress response of barley: gene networks reconstruction, hub, and promoter analysis

Endophyte Inoculation and Elevated Potassium Supply on Productivity, Growth and Physiological Parameters of Spring Barley (Hordeum vulgare L.) Genotypes over Contrasting Seasons

Plant Defence Induction by Meyerozyma guilliermondii in Vitis vinifera L.

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