Dynamic Transcriptional Changes of TIEG1 and TIEG2 During Mouse Tissue Development

Abstract

TGF-b-inducible early-response gene (TIEG) is a family of primary response genes induced by TGF-b, which are well recognized in regulating cellular proliferation and apoptosis. However, their expression profile has never been investigated during embryogenesis in different organs. In this study, we aimed to investigate the transcriptional level of both TIEG1 and TIEG2 during development in various mice organs, including the brain cortex, cerebellum and stem, brain striatum, muscle, heart, liver, kidney, and lung. Quantitative real-time PCR was used to profile the change of transcriptional level of the two TIEG members in the mice tissues at six developmental stages. Taken together, the expression of TIEG1 and TIEG2 was specific in different organs yet varied with different developmental time points. Their dynamic changes were moderately consistent in most organs including the brain cortex, striatum, liver, kidney, and lung. However, their mRNA expression in both the heart and muscle was significantly different at all developmental stages, which might propose a compensation of functions in the TIEG family. Nevertheless, our data indicate that both the TIEG genes are essential in regulating the normal organ development and functioning in murine model, as their expressions were ubiquitous and tissue specific at various developmental stages. Anat Rec,

TGFβ2 regulates hypothalamic Trh expression through the TGFβ inducible early gene-1 (TIEG1) during fetal development

“…The effect of TIEG1 on gene regulation is mediated by its interaction with co-repressor molecules, such as the histone deacetylase mSin3A (Zhang et al, 2001) and the histone demethylase JARID1B (Kim et al, 2010), or with the co-activator histone acetyltransferase p300/CBP-associated factor (PCAF) (Xiong et al, 2012). TIEG1 has a widespread expression pattern (Subramaniam et al, 2005); its expression is detected in various regions of the developing and adult murine brain (Yajima et al, 1997), with TIEG1 mRNA levels peaking a few days after birth in the mouse brain (Jiang et al, 2010). Multiple signaling molecules increase TIEG1 levels in the central nervous system (Consales et al, 2007; Wibrand et al, 2006).…”