Dynamic tissue perfusion measurement in the reproductive organs of the female and male dogs

Dzięcioł, Michał; Scholbach, Thomas; Stańczyk, Ewa; Ostrowska, Justyna; Kinda, Wojciech; Woźniak, Magdalena; Atamaniuk, Wojciech; Skrzypczak, Piotr; Niżański, Wojciech; Wieczorek, Paweł; Scholbach, J.; Kiełbowicz, Zdzisław

doi:10.2478/bvip-2014-0023

Cited by 10 publications

(10 citation statements)

References 41 publications

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“…In human and veterinary medicine, Doppler sonography has become a common noninvasive method to study perfusion characteristics of the female reproductive tract. Different Doppler methods, i. e. color (10), power (35) and pulse wave Doppler (14) have been tested and proven feasible for reproductive purposes when applied either transvaginally such as in women (33), transabdominally such as in bitches (2) or transrectally e. g. in cows and mares (5,35). Among large animal species, it is particularly the mare's repro ductive tract that has been intensively studied by Doppler sono graphy in order to characterize the uterine blood flow during the estrous cycle or to investigate perfusion patterns of the diseased uterus (6,11).…”

Section: Introductionmentioning

confidence: 99%

Feasibility of transabdominal Doppler sonography for studying uterine blood flow characteristics in cycling gilts

Herlt¹,

Stark²,

Sigmarsson³

et al. 2018

Tierarztl Prax Ausg G

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Summary Objective: To test for the feasibility of transabdominal Doppler sonography (color, power, pulse wave) to define uterine perfusion characteristics throughout the estrous cycle in gilts. Materials and methods: A total of 15 gilts were synchronized for estrus and scanned in their following spontaneous cycle while being restrained in a purpose-designed mobile crate. To define uterine perfusion characteristics, vessels in between and within uterine cross-sections were imaged and recorded as video sequences to be analyzed by PixelFlux® software for perfused area (Amix), blood flow velocity (vmix) and intensity (Imix) as well as resistance (RIvmix) and pulsatility index (PIvmix). Results: Color Doppler sonography proved to be the only feasible technique, as it was less affected by animal movements than power and pulse wave sonography. As determined by color Doppler sonography, all five parameters determined showed specific patterns through the estrous cycle, i. e. Amix, vmix, Imix were high in proestrus, decreased in estrus and remained low in midestrus and most parts of diestrus; RIvmix and PIvmix with inversely paralleled patterns. Conclusion and clinical relevance: This study has demonstrated that transabdominal color Doppler but not power and pulse wave Doppler sonography is feasible to be performed in crate-restrained gilts for studying uterine perfusion characteristics during the estrous cycle, and that changes of uterine perfusion over the course of the estrous cycle can be clearly followed by color Doppler sonography. Results encourage the use of color Doppler sonography for studying i. e. uterine capacity or uterus related infertility such as for cases of clinically unapparent endometritis.

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Section: Introductionmentioning

confidence: 99%

Feasibility of transabdominal Doppler sonography for studying uterine blood flow characteristics in cycling gilts

Herlt¹,

Stark²,

Sigmarsson³

et al. 2018

Tierarztl Prax Ausg G

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“…In animals, colour Doppler ultrasonography is generally used in cardiac and vascular assessments (King, 2006). In addition, colour Doppler ultrasonography has been reported to be useful for examining blood flow to genital organs in the whole reproductive period (Dzięcioł et al ., 2014; Bollwein et al ., 2016). To the best of our knowledge, there are no previously-published studies in the literature about the evaluation of supramammary lymph nodes in cows using the colour Doppler ultrasonography methods.…”

Section: Discussionmentioning

confidence: 99%

The relationship between mastitis and the B-mode, colour Doppler ultrasonography measurements of supramammary lymph nodes in cows

Rişvanlı

Doğan

Safak

et al. 2019

Journal of Dairy Research

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This research communication aims to test the hypothesis that B-Mode, colour Doppler ultrasonographic measurements and characteristics can identify mastitis in dairy cows. A total of 102 lactating cows were divided into 3 groups: cows in which all mammary quarters were CMT-negative, cows with CMT-positive mammary quarters and cows with clinical mastitis in at least one quarter. Colour Doppler ultrasonography measurements of the supramammary lymph nodes revealed that distortion-type vascular morphology, the rate of type 4 vascular densities and the incidence of mixed-type vascular distributions were highest in the clinical mastitis group, whereas the frequency of avascularity in supramammary lymph nodes was highest in the CMT-negative group. All differences were significant. In conclusion, the use of B-mode and colour Doppler ultrasonographic measurements of the supramammary lymph nodes can provide useful information about the current condition of mastitis in cows, although its diagnostic potential remains to be determined.

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“…Blood flow was evaluated by measurements of the velocity and intensity in the defined region of interest (ROI). The mean flow intensity (I) inside a ROI as well as the mean flow velocity (V) was calculated according the procedures described by Dzięcioł et al (14).…”

Section: Methodsmentioning

confidence: 99%

“…It also makes it possible to detect more subtle changes in perfusion of particular parts of the organs (13,14). Previously the lack of possibilities of objective evaluation of results obtained with the Doppler examination significantly reduced its usefulness for scientific research.…”

Section: Introductionmentioning

confidence: 99%

“…Previously the lack of possibilities of objective evaluation of results obtained with the Doppler examination significantly reduced its usefulness for scientific research. Currently, however, the new Pixel Flux (PXFX) software designed for dynamic measurements of tissue and organ perfusion allows objective evaluation of results obtained with this technique, and for the past few years it has also been successfully applied in veterinary medicine (14).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Intravaginal Administration of Sildenafil Citrate Increases Blood Flow in the Bovine Uterus

Dzięcioł

Noszczyk-Nowak

Michlik

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to evaluate the influence of sildenafil citrate administrated intravaginaly on the blood flow in the bovine uterus during dioestrus. Uterine blood flow was examined in six healthy adult cows. Sildenafil was administrated intravaginaly to each cow between the 6 th and 8 th d of the ovarian cycle, in the form of vaginal suppositories containing 100 mg of active substance at a dose of 100, 200, or 300 mg per animal. Uterine perfusion was estimated by the colour Doppler examination, and obtained results were analysed with the Pixel Flux Software (Chameleon, Germany). Moreover, cardiovascular parameters were also evaluated. Animals were examined before and five times after drug application (two times at 15 min intervals, and three times at 2 h intervals). A placebo suppository was also given to the cows. The analysis of the intensity and velocity of blood flow in the uterus proved that sildenafil administrated intravaginaly significantly incre ased blood flow in the uterus and the effect of increased perfusion was observed for 4 h and 30 min after administration. The effect of increased uterine perfusion was observed after low as well as high doses of sildenafil. Significant changes in the cardiovascular parameters were not detected. There were no changes in the uterine perfusion as well as in cardiovascular parameters after placebo administration.

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Dynamic tissue perfusion measurement in the reproductive organs of the female and male dogs

Cited by 10 publications

References 41 publications

Feasibility of transabdominal Doppler sonography for studying uterine blood flow characteristics in cycling gilts

Feasibility of transabdominal Doppler sonography for studying uterine blood flow characteristics in cycling gilts

The relationship between mastitis and the B-mode, colour Doppler ultrasonography measurements of supramammary lymph nodes in cows

Intravaginal Administration of Sildenafil Citrate Increases Blood Flow in the Bovine Uterus

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