Dynamic thiol/disulfide homeostasis in children with community‐acquired pneumonia

Abstract

Background Alteration in thiol level under oxidative stress may contribute to community‐acquired pneumonia (CAP). The goal of this study was to determine whether there are changes in thiol/disulfide homeostasis and nitric oxide (NO) in children with CAP. Methods In total, 130 participants were involved in the study. Of these, 65 had been diagnosed with CAP on admission, and the remaining 65 were healthy individuals. Serum total thiol and native thiol were measured in each participant using a novel automated spectrophotometric method. The amount of dynamic disulfide bonds and related ratios were calculated from these values. Serum NO was measured on chemiluminescence assay. Results Average native thiol, total thiol, and disulfide in the CAP group were significantly lower than in the healthy individuals (P < 0.0001, P < 0.0001, P = 0.0126, respectively). In addition, disulfide/native thiol (P = 0.0002), and disulfide/total thiol ratios (P = 0.0004) were significantly higher, whereas the native thiol/total thiol ratio (P = 0.0004) was lower in the CAP group. High serum NO was noted in the CAP group (P = 0.0003), but there was no marked correlation between thiol/disulfide and NO. Conclusion The changes in endogenous thiol levels under oxidative stress may be associated with the pathogenesis of CAP in pediatric patients.

Investigation of Dynamic Thiol Disulfide Homeostasis in Acute Respiratory Failure Patients in Intensive Care Unit

“…In two different studies conducted in adult patients hospitalized with the diagnosis of community-acquired pneumonia and in the pediatric patient group, total thiol and native thiol levels have been found to be significantly lower in patients compared to the control group (27,31). These findings are consistent with the findings we obtained in our study.…”

“…This can be interpreted as that antioxidant mechanisms could not participate in the oxidative stress process in time, as our study group consisted of patients with acute respiratory failure. In another study conducted by Temel et al, consistent with our findings, it has been found that native thiol, total thiol, and disulfide levels to be lower in children with community-acquired pneumonia than in the control group (31). However, unlike this study, they have found the ratio of native thiol / total thiol to be lower, and the disulfide / native thiol and disulfide/total thiol ratio to be higher than the control group.…”

Thiol-disulfide homeostasis in children with febrile neutropenia

“…Multiple types of research on advanced oxidation protein final products in various diseases have been conducted. These studies found that children with infectious diseases had higher disulfide/ native thiol ratios and disulfide/total thiol ratios and reduced native thiol, total thiol levels, and native thiol/total thiol ratios (13)(14)(15)(16)(17)(18)(19)(20)(21)25,26). Because of these changes in the thiol-disulfide balance, it may be a completely new marker for determining the severity of febrile neutropenia in ALL patients.…”

“…It was discovered that oxidative stress was linked to infectious diseases such as hepatitis C, pneumonia, Brucellosis, sepsis, appendicitis, urinary tract infection, and tonsillopharyngitis (13)(14)(15)(16)(17)(18)(19)(20)(21). Thiol levels have recently been regarded as an antioxidant measure in various disorders.…”

The role of serum HLD, LDL, and uric acid levels in predicting the prognosis of community-acquired pneumonia

“…Community-acquired pneumonia (CAP) is a clinical condition caused by infectious diseases and also a leading cause of mortality and morbidity. CAP is often accompanied by infection-associated inflammation and oxidative stress [5]. To date, a variety of scoring systems [(Confusion Urea Respiratory Rate Blood Pressure-65 (CURB-65) and pneumonia severity index (PSI)] and biochemical markers (c-reactive protein, procalcitonin) have been used in the assessment of hospitalization, mortality and prognosis in CAP patients.…”