Dynamic <sup>31</sup>P–MRSI using spiral spectroscopic imaging can map mitochondrial capacity in muscles of the human calf during plantar flexion exercise at 7 T

Valkovič, Ladislav; Chmelík, Marek; Meyerspeer, Martin; Gagoski, Borjan; Rodgers, Christopher T.; Krššák, Martin; Andronesi, Ovidiu C.; Trattnig, Siegfried; Bogner, Wolfgang

doi:10.1002/nbm.3662

Cited by 38 publications

(55 citation statements)

References 55 publications

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“…Moreover, additional measurements with increased force and a flexed knee were attempted, but did not generate similar depletion to that in gastrocnemius medialis or were not well tolerated by the subject. The observation that the PCr depletion in soleus was stronger with a flexed knee than with a straight leg, but without depletion reaching the maximum depletion levels of gastrocnemius, has also been reported by Valkovic et al…”

Section: Discussionsupporting

confidence: 73%

“…A relation between knee angle and the recruitment pattern of gastrocnemius and soleus is expected according to anatomy and has been explored with 1 H MRI and 31 P MRS . The purpose of this study was to investigate the effect of knee angle on metabolic changes in those muscles in further detail, in order to establish a more comprehensive model by acquiring localized 31 P MRS data in exercising calf muscles with a simple and time‐efficient method with four different knee angles.…”

Section: Discussionmentioning

confidence: 99%

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Dynamic multivoxel‐localized ³¹P MRS during plantar flexion exercise with variable knee angle

et al. 2018

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Exercise studies investigating the metabolic response of calf muscles using 31P MRS are usually performed with a single knee angle. However, during natural movement, the distribution of workload between the main contributors to force, gastrocnemius and soleus is influenced by the knee angle. Hence, it is of interest to measure the respective metabolic response of these muscles to exercise as a function of knee angle using localized spectroscopy.Time‐resolved multivoxel 31P MRS at 7 T was performed simultaneously in gastrocnemius medialis and soleus during rest, plantar flexion exercise and recovery in 12 healthy volunteers. This experiment was conducted with four different knee angles.PCr depletions correlated negatively with knee angle in gastrocnemius medialis, decreasing from 79±14 % (extended leg) to 35±23 %(∼40°), and positively in soleus, increasing from 20±21 % to 36±25 %; differences were significant. Linear correlations were found between knee angle and end‐exercise PCr depletions in gastrocnemius medialis (R 2=0.8) and soleus (R 2=0.53). PCr recovery times and end‐exercise pH changes that correlated with PCr depletion were consistent with the literature in gastrocnemius medialis and differences between knee angles were significant. These effects were less pronounced in soleus and not significant for comparable PCr depletions. Maximum oxidative capacity calculated for all knee angles was in excellent agreement with the literature and showed no significant changes between different knee angles.In conclusion, these findings confirm that plantar flexion exercise with a straight leg is a suitable paradigm, when data are acquired from gastrocnemius only (using either localized MRS or small surface coils), and that activation of soleus requires the knee to be flexed. The present study comprises a systematic investigation of the effects of the knee angle on metabolic parameters, measured with dynamic multivoxel 31P MRS during muscle exercise and recovery, and the findings should be used in future study design.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Dynamic multivoxel‐localized ³¹P MRS during plantar flexion exercise with variable knee angle

et al. 2018

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“…Recently, a constant-density spiral spectroscopic readout has been proposed for dynamic 31 P-MRSI experiments [261]. The use of temporal interleaved spirals allowed the acquisition of a 14 × 14 matrix in 10 s; thus, a simple measurement protocol using repetitive exercise can be used.…”

Section: Localization Of 31p-mr Signal For Investigations Of Skeletalmentioning

confidence: 99%

In-vivo 31P-MRS of skeletal muscle and liver: A way for non-invasive assessment of their metabolism

Valkovič

Chmelík

Krššák

2017

Analytical Biochemistry

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In addition to direct assessment of high energy phosphorus containing metabolite content within tissues, phosphorus magnetic resonance spectroscopy (31P-MRS) provides options to measure phospholipid metabolites and cellular pH, as well as the kinetics of chemical reactions of energy metabolism in vivo. Even though the great potential of 31P-MR was recognized over 30 years ago, modern MR systems, as well as new, dedicated hardware and measurement techniques provide further opportunities for research of human biochemistry. This paper presents a methodological overview of the 31P-MR techniques that can be used for basic, physiological, or clinical research of human skeletal muscle and liver in vivo. Practical issues of 31P-MRS experiments and examples of potential applications are also provided. As signal localization is essential for liver 31P-MRS and is important for dynamic muscle examinations as well, typical localization strategies for 31P-MR are also described.

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“…These processes have been investigated in several studies employing either 31 P MRS and MRI or 1 H arterial spin labeling (ASL) MRI in calf muscles during and after exercise. 1 H ASL is an approach to noninvasively assess local perfusion rates, while 31 P MRS is a well‐established method to investigate the energy metabolism in vivo.…”

Section: Introductionmentioning

confidence: 99%

Interleaved ³¹P MRS/¹H ASL for analysis of metabolic and functional heterogeneity along human lower leg muscles at 7T

Niess

Schmid

Bogner

et al. 2019

Magnetic Resonance in Med

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Purpose MR offers the unique possibility to noninvasively investigate cellular energy metabolism via 31P MRS, while blood perfusion, which provides oxygen and substrates to the tissue, is accessible by arterial spin labeling (ASL) 1H MRI. Because metabolic and hemodynamic parameters are linked, it would be desirable to study them simultaneously. A 3D‐resolved method is presented that allows such measurements with high spatiotemporal resolution and has the potential to discern differences along an exercising muscle. Methods Multi‐voxel localized 31P MRS was temporally interleaved with multi‐slice pASL 1H MRI. Phosphorus spectra were collected from two adjacent positions in gastrocnemius medialis (GM) during rest, submaximal plantar flexion exercise and recovery, while perfusion and T2*‐weighted axial images were acquired at the same time. Seventeen healthy volunteers (9 f / 8 m) were studied at 7 T. Results An increase of postexercise perfusion and T2*‐weighted signal in GM positively correlated with end‐exercise PCr depletion and pH drop. At proximal positions functional and metabolic activity was higher than distally, that is, perfusion increase and peak T2*‐weighted signal, end‐exercise PCr depletion, end‐exercise pH, and PCr recovery time constant were significantly different. An NOE‐induced SNR increase of approximately 20 % (P < .001), at rest, was found in interleaved 31P spectra, when comparing to 31P‐only acquisitions. Conclusions A technique for fast, simultaneous imaging of muscle functional heterogeneity in ASL, T2* and acquisition of time‐resolved 31P MRS data is presented. These single exercise recovery experiments can be used to investigate local variations during disease progression in patients suffering from vascular or muscular diseases.

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Dynamic ³¹P–MRSI using spiral spectroscopic imaging can map mitochondrial capacity in muscles of the human calf during plantar flexion exercise at 7 T

Cited by 38 publications

References 55 publications

Dynamic multivoxel‐localized ³¹P MRS during plantar flexion exercise with variable knee angle

Dynamic multivoxel‐localized ³¹P MRS during plantar flexion exercise with variable knee angle

In-vivo 31P-MRS of skeletal muscle and liver: A way for non-invasive assessment of their metabolism

Interleaved ³¹P MRS/¹H ASL for analysis of metabolic and functional heterogeneity along human lower leg muscles at 7T

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Dynamic 31P–MRSI using spiral spectroscopic imaging can map mitochondrial capacity in muscles of the human calf during plantar flexion exercise at 7 T

Cited by 38 publications

References 55 publications

Dynamic multivoxel‐localized 31P MRS during plantar flexion exercise with variable knee angle

Dynamic multivoxel‐localized 31P MRS during plantar flexion exercise with variable knee angle

In-vivo 31P-MRS of skeletal muscle and liver: A way for non-invasive assessment of their metabolism

Interleaved 31P MRS/1H ASL for analysis of metabolic and functional heterogeneity along human lower leg muscles at 7T

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Dynamic ³¹P–MRSI using spiral spectroscopic imaging can map mitochondrial capacity in muscles of the human calf during plantar flexion exercise at 7 T

Dynamic multivoxel‐localized ³¹P MRS during plantar flexion exercise with variable knee angle

Dynamic multivoxel‐localized ³¹P MRS during plantar flexion exercise with variable knee angle

Interleaved ³¹P MRS/¹H ASL for analysis of metabolic and functional heterogeneity along human lower leg muscles at 7T