Dynamic subcellular localization of estrogen receptor alpha during the first two cleavages of mouse preimplantation embryos

Phosphorylation site S122 in estrogen receptor α has a tissue‐dependent role in female mice

“…Furthermore, the importance of this phosphorylation site has been proven by in vitro studies in several different cell types including COS‐1 cells (a monkey kidney cell line), HeLa cells (a human cervical cancer cell line), and MCF‐7 cells (a human breast cancer cell line) in which serine 118 has been changed into alanine, an amino acid that cannot be phosphorylated 32,42 . The presence of phosphorylation of serine 122 (S122) in mouse has been demonstrated using phospho‐specific antibodies, 43‐46 and the serine to alanine substitution at site 122 in the murine ERα (S122A) has been shown to affect the signaling of the receptor in vitro 47 . Interestingly, the inability of the human ERα to be phosphorylated at site 118 leads to decreased estradiol‐induced gene transcription in some, 34 but not all, 48 cell‐types.…”

Atypical GATA protein TRPS1 plays indispensable roles in mouse two-cell embryo

“…Besides, TRPS1 is highly expressed in estrogen receptor-positive (ER + ) breast cancer cells [36,37]. It was also found to recruit histone deacetylase (HDAC) complexes to enhancers and act as a key repressor of ERα [38], which was dynamically expressed during mouse PED [39][40][41] and affected the expression of MuERV-L at ZGA [42]. Furthermore, through regulating the expression of Gata3, ERα also functions in blastocyst formation [43].…”