Dynamic study of cell mechanical and structural responses to rapid changes of calcium level

Richelme, Fabienne; Benoliel, Anne‐Marie; Bongrand, Pierre

doi:10.1002/(sici)1097-0169(200002)45:2<93::aid-cm2>3.0.co;2-z

Cited by 22 publications

(25 citation statements)

References 75 publications

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“…In agreement with our results, it was previously observed that microfilament relocation did not affect the amount of F‐actin [42,43]. Also, the influence of Ca 2+ on the actin cytoskeleton disposition has already been shown in experiments where a rise in cytosolic Ca 2+ generated a displacement of peripheral actin to the inner cell regions without changes in F‐actin content in human monocytic THP1 cells [44]. It is possible that the level of cytosolic Ca 2+ needs to reach a threshold in the cells to trigger F‐actin reorganization.…”

Section: Discussionsupporting

confidence: 92%

The cytoskeleton, a structure that is susceptible to the toxic mechanism activated by palytoxins in human excitable cells

Louzao¹,

Ares²,

Vieytes³

et al. 2007

The FEBS Journal

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Palytoxin is a marine toxin responsible for a fatal type of poisoning in humans named clupeotoxism, with symptoms such as neurologic disturbances. It is believed that it binds to the Na+/K+‐ATPase from the extracellular side and modifies cytosolic ions; nevertheless, its effects on internal cell structures, such as the cytoskeleton, which might be affected by these initial events, have not been fully elucidated. Likewise, ostreocin‐D, an analog of palytoxin, has been only recently found, and its action on excitable cells is therefore unknown. Therefore, our aim was to investigate the modifications of ion fluxes associated with palytoxin and ostreocin‐D activities, and their effects on an essential cytoskeletal component, the actin system. We used human neuroblastoma cells and fluorescent dyes to detect changes in membrane potential, intracellular Ca2+ concentration, cell detachment, and actin filaments. Fluorescence values were obtained with spectrofluorymetry, laser‐scanning cytometry, and confocal microscopy; the last of these was also used for recording images. Palytoxin and ostreocin‐D modified membrane permeability as a first step, triggering depolarization and increasing Ca2+ influx. The substantial loss of filamentous actin, and the morphologic alterations elicited by both toxins, are possibly secondary to their action on ion channels. The decrease in polymerized actin seemed to be Ca2+‐independent; however, this ion could be related to actin cytoskeletal organization. Palytoxin and ostreocin‐D alter the ion fluxes, targeting pathways that involve the cytoskeletal dynamics of human excitable cells.

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Section: Discussionsupporting

confidence: 92%

The cytoskeleton, a structure that is susceptible to the toxic mechanism activated by palytoxins in human excitable cells

Louzao¹,

Ares²,

Vieytes³

et al. 2007

The FEBS Journal

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“…Our study supports the hypothesis that the observed changes in chondrocyte viscoelastic properties with hypoosmotic stress are attributable to a dissociation or redistribution of the F-actin cytoskeleton. The mechanisms leading to this event are not fully understood but potentially involve a transient increase in the intracellular concentration of Ca 2ϩ Richelme et al, 2000). These findings suggest that changes occurring in the physicochemical environment of the chondrocyte in situ because of deformation of the extracellular matrix may directly influence the cytoskeletal structure and viscoelastic properties of the cell, therefore altering the biomechanical interactions between the chondrocyte and its extracellular matrix (Guilak and Mow, 2000).…”

Section: Discussionmentioning

confidence: 99%

The Effects of Osmotic Stress on the Viscoelastic and Physical Properties of Articular Chondrocytes

Guilak

Erickson

Ting‐Beall

2002

Biophysical Journal

226

202

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The metabolic activity of chondrocytes in articular cartilage is influenced by alterations in the osmotic environment of the tissue, which occur secondary to mechanical compression. The mechanism by which osmotic stress modulates cell physiology is not fully understood and may involve changes in the physical properties of the membrane or the cytoskeleton. The goal of this study was to determine the effect of the osmotic environment on the mechanical and physical properties of chondrocytes. In isoosmotic medium, chondrocytes exhibited a spherical shape with numerous membrane ruffles. Normalized cell volume was found to be linearly related to the reciprocal of the extracellular osmolality (Boyle van't Hoff relationship) with an osmotically active intracellular water fraction of 61%. In deionized water, chondrocytes swelled monotonically until lysis at a mean apparent membrane area 234 +/- 49% of the initial area. Biomechanically, chondrocytes exhibited viscoelastic solid behavior. The instantaneous and equilibrium elastic moduli and the apparent viscosity of the cell were significantly decreased by hypoosmotic stress, but were unchanged by hyperosmotic stress. Changes in the viscoelastic properties were paralleled by the rapid dissociation and remodeling of cortical actin in response to hypoosmotic stress. These findings indicate that the physicochemical environment has a strong influence on the viscoelastic and physical properties of the chondrocyte, potentially through alterations in the actin cytoskeleton.

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“…The method allows for the measurement of cellular elasticity and viscosity, as well as mechanical tension in the cellular membrane. Micropipette aspiration has been used to study the mechanical properties of monocytes, red blood cells, leukocytes, and erythrocytes (Discher et al 1998, Dong et al 1988, Hochmuth 2000, Richelme et al 2000.…”

Section: Micropipette Aspirationmentioning

confidence: 99%

Rheology of the Cytoskeleton

Mofrad

2009

Annu. Rev. Fluid Mech.

115

109

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The cytoskeleton is the primary internal structure of the cell, providing its structural integrity. The rheology and mechanics of the cytoskeleton, therefore, are key to the cell's ability to accomplish its diverse functions in health and disease. Although the importance of the cytoskeleton is well established, the relationship between the microstructural details and the macroscopic rheological behavior of the cytoskeleton remains elusive. A wide range of computational and phenomenological models as well as experimental techniques have been proposed over the past two decades to describe the cytoskeleton, giving rise to several, often contradictory, theories for describing its rheology. This concise review attempts to bring together the key experimental methods and theoretical and computational models regarding cytoskeletal rheology and mechanics.

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Dynamic study of cell mechanical and structural responses to rapid changes of calcium level

Cited by 22 publications

References 75 publications

The cytoskeleton, a structure that is susceptible to the toxic mechanism activated by palytoxins in human excitable cells

The cytoskeleton, a structure that is susceptible to the toxic mechanism activated by palytoxins in human excitable cells

The Effects of Osmotic Stress on the Viscoelastic and Physical Properties of Articular Chondrocytes

Rheology of the Cytoskeleton

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