Dynamic Selenium Assimilation, Distribution, Efflux, and Maternal Transfer in Japanese Medaka Fed a Diet of Se-enriched Mayflies

Conley, Justin M.; Watson, AtLee T D; Xie, Lingtian; Buchwalter, David B.

doi:10.1021/es404933t

Cited by 26 publications

(19 citation statements)

References 37 publications

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“…In medaka, the process of vitellogenesis and associated oocyte maturation of individual follicles requires 72 hours (Kinoshita et al, 2009; Shibata et al, 2011). During this time, immediate diet supplies nutrients and trace elements to maturing oocytes, underscoring the linkage of recent dietary consumption to observable results in short time scales (Conley et al, 2014). …”

Section: Introductionmentioning

confidence: 97%

“…Although hatchability of embryos is generally not affected (Hamilton, 2004; Woock et al, 1987), deformities become evident with development, impairing critical body functions (Lemly, 1997c, 2002a). In medaka ( Oryzias latipes ), maternal transfer was followed by use of 75 Se to determine rapid movement of dietary Se to ovary and its maturing eggs (Conley et al, 2014). To date, most field and laboratory Se toxicity studies concentrate on maternal exposure.…”

Section: Introductionmentioning

confidence: 99%

“…They provide for careful control of exposure levels of toxicants in food and water while transparent chorions enable detailed observations and evaluation of development (Iwamatsu, 2004; Takeda and Shimada, 2010; Westerfield, 2000). Recent Se dietary studies have used these models to good advantage (Conley et al, 2014; Thomas and Janz, 2014). Because these species breed asynchronously, the timing of exposure is important.…”

Section: Introductionmentioning

confidence: 99%

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Parental dietary seleno-L-methionine exposure and resultant offspring developmental toxicity

et al. 2016

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Selenium (Se) leaches into water from agricultural soils and from storage sites for coal fly ash. Se toxicity causes population and community level effects in fishes and birds. We used the laboratory aquarium model fish, Japanese medaka (Oryzias latipes), an asynchronous breeder, to determine aspects of uptake in adults and resultant developmental toxicity in their offspring. The superior imaging properties of the model enabled detailed descriptions of phenotypic alterations not commonly reported in the existing Se literature. Adult males and females in treatment groups were exposed, separately and together, to a dry diet spiked with 0, 12.5, 25, or 50 µg/g (dry weight) seleno-L-methionine (SeMet) for 6 days, and their embryo progeny collected for 5 days, maintained under controlled conditions and observed daily for hatchability, mortality and/or developmental toxicity. Sites of alteration included: craniofacial, pericardium and abdomen (Pc/Ab), notochord, gall bladder, spleen, blood, and swim bladder. Next, adult tissue Se concentrations (liver, skeletal muscle, ovary and testis) were determined and compared in treatment groups of bred and unbred individuals. No significant difference was found across treatment groups at the various SeMet concentrations; and, subsequent analysis compared exposed vs. control in each of the treatment groups at 10 dpf. Increased embryo mortality was observed in all treatment groups, compared to controls, and embryos had a decreased hatching rate when both parents were exposed. Exposure resulted in significantly more total altered phenotypes than controls. When altered phenotypes following exposure of both parents were higher than maternal only exposure, a male role was suggested. The comparisons between treatment groups revealed that particular types of phenotypic change may be driven by the sex of the exposed parent. Additionally, breeding reduced Se concentrations in some adult tissues, specifically the liver of exposed females and skeletal muscle of exposed males. Detailed phenotypic analysis of progeny from SeMet exposed parents should inform investigations of later life stages in an effort to determine consequences of early life exposure.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Parental dietary seleno-L-methionine exposure and resultant offspring developmental toxicity

et al. 2016

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“…This parthenogenetic species (see Funk et al, 2006) has emerged as a useful laboratory model for ecological (Sweeney and Vannote, 1984) and physiological/toxicological studies (Sweeney et al, 1993;Conley et al, 2009Conley et al, , 2011Conley et al, , 2013Conley et al, , 2014Kim et al, 2012;Kunz et al, 2013;Soucek and Dickinson, 2015;Xie et al, 2010;Xie and Buchwalter, 2011;Johnson et al, 2015). We established chronic upper thermal limits by rearing newly hatched eggs to adulthood across several temperatures.…”

Section: Introductionmentioning

confidence: 99%

Physiological responses to short-term thermal stress in mayfly (Neocloeon triangulifer) larvae in relation to upper thermal limits

Kim

Chou

Funk

et al. 2017

Journal of Experimental Biology

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Understanding species' thermal limits and their physiological determinants is critical in light of climate change and other human activities that warm freshwater ecosystems. Here, we ask whether oxygen limitation determines the chronic upper thermal limits in larvae of the mayfly Neocloeon triangulifer, an emerging model for ecological and physiological studies. Our experiments are based on a robust understanding of the upper acute (∼40°C) and chronic thermal limits of this species (>28°C, ≤30°C) derived from full life cycle rearing experiments across temperatures. We tested two related predictions derived from the hypothesis that oxygen limitation sets the chronic upper thermal limits: (1) aerobic scope declines in mayfly larvae as they approach and exceed temperatures that are chronically lethal to larvae; and (2) genes indicative of hypoxia challenge are also responsive in larvae exposed to ecologically relevant thermal limits. Neither prediction held true. We estimated aerobic scope by subtracting measurements of standard oxygen consumption rates from measurements of maximum oxygen consumption rates, the latter of which was obtained by treating with the metabolic uncoupling agent carbonyl cyanide-4-(trifluoromethoxy) pheylhydrazone (FCCP). Aerobic scope was similar in larvae held below and above chronic thermal limits. Genes indicative of oxygen limitation (LDH, EGL-9) were only upregulated under hypoxia or during exposure to temperatures beyond the chronic (and more ecologically relevant) thermal limits of this species (LDH). Our results suggest that the chronic thermal limits of this species are likely not driven by oxygen limitation, but rather are determined by other factors, e.g. bioenergetics costs. We caution against the use of short-term thermal ramping approaches to estimate critical thermal limits (CT max ) in aquatic insects because those temperatures are typically higher than those that occur in nature.

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“…Despite their ecological importance and use in monitoring programs, mayfly physiology remains poorly studied because relative few species are amenable to laboratory rearing. Neocloeon triangulifer is a parthenogenetic species that is emerging as a useful laboratory model (Weaver et al, 2015) for understanding responses to environmental stressors such as contaminants (Conley et al, 2009; Conley et al, 2011; Xie and Buchwalter, 2011; Kim et al, 2012; Conley et al, 2013; Kunz et al, 2013; Conley et al, 2014; Wesner et al, 2014; Lopez et al, 2016), salinity stress (Johnson et al, 2014; Soucek and Dickinson, 2015; Struewing et al, 2015) and temperature (Sweeney and Vannote, 1984). …”

Section: Introductionmentioning

confidence: 99%

Metabolomics reveal physiological changes in mayfly larvae (Neocloeon triangulifer) at ecological upper thermal limits

Chou

Pathmasiri

Deese-Spruill

et al. 2017

Journal of Insect Physiology

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Aquatic insects play critical roles in freshwater ecosystems and temperature is a fundamental driver of species performance and distributions. However, the physiological mechanisms that determine the thermal performance of species remain unclear. Here we used a metabolomics approach to gain insights into physiological changes associated with a short-term, sublethal thermal challenge in the mayfly Neocloeon triangulifer (Ephemeroptera: Baetidae). Larvae were subjected to a thermal ramp (from 22 to 30 °C at a rate of 1°C/h) and metabolomics analysis (both Nuclear Magnetic Resonance (NMR) Spectroscopy and Gas Chromatography coupled Time-of-Flight Mass Spectrometry (GC-TOF-MS)) indicated that processes related to energetics (sugar metabolism) and membrane stabilization primarily differentiated heat treated larvae from controls. Limited evidence of anaerobic metabolism was observed in the heat treated larvae at 30°C, a temperature that is chronically lethal to larvae.

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Dynamic Selenium Assimilation, Distribution, Efflux, and Maternal Transfer in Japanese Medaka Fed a Diet of Se-enriched Mayflies

Cited by 26 publications

References 37 publications

Parental dietary seleno-L-methionine exposure and resultant offspring developmental toxicity

Parental dietary seleno-L-methionine exposure and resultant offspring developmental toxicity

Physiological responses to short-term thermal stress in mayfly (Neocloeon triangulifer) larvae in relation to upper thermal limits

Metabolomics reveal physiological changes in mayfly larvae (Neocloeon triangulifer) at ecological upper thermal limits

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