Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation

Li, Jianjia; Wang, Zhifa; Huang, Xiangyu; Wang, Zhaodan; Chen, Zehao; Wang, Runting; Zhao, Chen; Liu, Wei; Wu, Buling; Fang, Fuchun; Qiu, Wei

doi:10.1186/s13287-020-02123-6

Cited by 20 publications

(21 citation statements)

References 90 publications

(66 reference statements)

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“…PDLSCs exhibit osteoblast-like characteristics and are able to differentiate into osteoblasts or osteoblasts. They are also considered as the optimal seed cells for periodontal regeneration [ 49 ].…”

Section: Pdlsc Sheets Applicationsmentioning

confidence: 99%

Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine

Chen

Huang

et al. 2022

Stem Cell Res Ther

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Stem cells transplantation is the main method of tissue engineering regeneration treatment, the viability and therapeutic efficiency are limited. Scaffold materials also play an important role in tissue engineering, whereas there are still many limitations, such as rejection and toxic side effects caused by scaffold materials. Cell sheet engineering is a scaffold-free tissue technology, which avoids the side effects of traditional scaffolds and maximizes the function of stem cells. It is increasingly being used in the field of tissue regenerative medicine. Dental-derived mesenchymal stem cells (DMSCs) are multipotent cells that exist in various dental tissues and can be used in stem cell-based therapy, which is impactful in regenerative medicine. Emerging evidences show that cell sheets derived from DMSCs have better effects in the field of regenerative medicine applications. Extracellular matrix (ECM) is the main component of cell sheets, which is a dynamic repository of signalling biological molecules and has a variety of biological functions and may play an important role in the application of cell sheets. In this review, we summarized the application status, mechanisms that sheets and ECM may play and future prospect of DMSC sheets on regeneration medicine.

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Section: Pdlsc Sheets Applicationsmentioning

confidence: 99%

Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine

Chen

Huang

et al. 2022

Stem Cell Res Ther

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“…For example, several studies have reported controversial results for the effects of HG on the differentiation of PDL cells (45)(46)(47). The overexpression of LIN28A enhances the osteogenic process and calcified nodule formation by increasing lactate and ATP production and decreasing reactive oxygen species production (30); furthermore, proteomic analysis of human PDL cells has identified OXPHOS as the most significantly upregulated pathway during their osteogenic differentiation (48). Elucidation of the dynamic metabolic preference of PDL cells during differentiation will presumably explain the discrepancy among several studies on the energy metabolism of PDL cells.…”

Section: Understanding Energy Metabolism In Pdl Cells: a Possible Strategy For Periodontal Regenerationmentioning

confidence: 99%

Energy Metabolism in Osteogenic Differentiation and Reprogramming: A Possible Future Strategy for Periodontal Regeneration

2022

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Energy metabolism is crucial in stem cells as they harbor various metabolic pathways depending on their developmental stages. Moreover, understanding the control of their self-renewal or differentiation via manipulation of their metabolic state may yield novel regenerative therapies. Periodontal ligament (PDL) cells existing between the tooth and alveolar bone are crucial for maintaining homeostasis in the periodontal tissue. In addition, they play a pivotal role in periodontal regeneration, as they possess the properties of mesenchymal stem cells and are capable of differentiating into osteogenic cells. Despite these abilities, the treatment outcome of periodontal regenerative therapy remains unpredictable because the biological aspects of PDL cells and the mechanisms of their differentiation remain unclear. Recent studies have revealed that metabolism and factors affecting metabolic pathways are involved in the differentiation of PDL cells. Furthermore, understanding the metabolic profile of PDL cells could be crucial in manipulating the differentiation of PDL cells. In this review, first, we discuss the energy metabolism in osteoblasts and stem cells to understand the metabolism of PDL cells. Next, we summarize the metabolic preferences of PDL cells during their maintenance and cytodifferentiation. The perspectives discussed have potential applicability for creating a platform for reliable regenerative therapies for periodontal tissue.

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“…These differences in salivary protein profiles can be used as disease indicators and help in the diagnosis [ 124 ]. Proteomic profiling of gingival crevicular fluid for disease biomarkers and osteogenic differentiation potential in periodontal ligament stem cells has provided new avenues for diagnosis and periodontal regeneration [ 125 , 126 ]. Evaluations and comparisons of the proteome profile could highlight alterations in diseased and healthy conditions and drive the discovery of prospective biomarkers.…”

Section: Proteomicsmentioning

confidence: 99%

Future Drug Targets in Periodontal Personalised Medicine—A Narrative Review

Yadalam

Varatharajan

Fageeh

et al. 2022

JPM

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Periodontal disease is an infection-driven inflammatory disease characterized by the destruction of tooth-supporting tissues. The establishment of chronic inflammation will result in progressive destruction of bone and soft tissue changes. Severe periodontitis can lead to tooth loss. The disease has complex pathogenesis with an interplay between genetic, environmental, and host factors and pathogens. Effective management consists of plaque control and non-surgical interventions, along with adjuvant strategies to control inflammation and disrupt the pathogenic subgingival biofilms. Recent studies have examined novel approaches for managing periodontal diseases such as modulating microbial signaling mechanisms, tissue engineering, and molecular targeting of host inflammatory substances. Mounting evidence suggests the need to integrate omics-based approaches with traditional therapy to address the disease. This article discusses the various evolving and future drug targets, including proteomics, gene therapeutics, vaccines, and nanotechnology in personalized periodontal medicine for the effective management of periodontal diseases.

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Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation

Cited by 20 publications

References 90 publications

Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine

Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine

Energy Metabolism in Osteogenic Differentiation and Reprogramming: A Possible Future Strategy for Periodontal Regeneration

Future Drug Targets in Periodontal Personalised Medicine—A Narrative Review

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