Dynamic Oxygen Enhances Oocyte Maturation in Long-Term Follicle Culture

Heise, Matthew K.; Koepsel, Richard R.; McGee, Elizabeth A.; Russell, Alan J.

doi:10.1089/ten.tec.2007.0418

Cited by 28 publications

(15 citation statements)

References 31 publications

(37 reference statements)

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“…4a), have been used by various researchers to prevent follicle adhesion to the substrate surface (Rowghani et al 2004;Heise et al 2005Heise et al , 2009). Rowghani et al (2004) described the culture of follicles in a suspension within rotating wall vessels, cylindrical chambers that rotate the medium axially (Fig.…”

Section: D In Vitro Follicle Culturementioning

confidence: 99%

Three-dimensional systems for in vitro follicular culture: overview of alginate-based matrices

Brito

Lima

et al. 2014

Reprod. Fertil. Dev.

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The in vitro culture of ovarian follicles has provided critical insight into the biology of the follicle and its enclosed oocyte and the physical interaction and communication between the theca and granulosa cells and the oocyte that is necessary to produce meiotically competent oocytes. Various two-dimensional (2D) and three-dimensional (3D) culture systems have been developed to evaluate the effect of growth factors, hormones, extracellular matrix components and culture conditions on follicle development and oocyte growth and maturation. Among these culture systems, 3D systems make it possible to maintain follicle structure and support communication between the various cell compartments within the follicle. In this review article, we will discuss the three main approaches to ovarian follicle culture: 2D attachment systems, 3D floating systems and 3D encapsulated systems. We will specifically emphasise the development of and advances in alginate-based encapsulated systems for in vitro follicle culture.

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Section: D In Vitro Follicle Culturementioning

confidence: 99%

Three-dimensional systems for in vitro follicular culture: overview of alginate-based matrices

Brito

Lima

et al. 2014

Reprod. Fertil. Dev.

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“…Medium-199 (Gibco, Paisley, UK), supplemented with 5% FCS and antibiotics, was used for culturing GCs. Although moderation of oxygen availability is key to obtaining follicle viability in vitro [ 18 , 19 ], the kinetics of oxygen concentration in the inner follicle, along with follicle development from early antral follicles to pre-ovulatory large follicles, is still unclear. In general, 5% and 20% oxygen tensions are used as physiological and atmospheric conditions, respectively, for the study of embryo culture [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of aging on mitochondria and metabolism of bovine granulosa cells

Nagata

Tatematsu

Kansaku

et al. 2020

Journal of Reproduction and Development

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This study investigated the effect of aging on mitochondria in granulosa cells (GCs) collected from the antral follicles of young and aged cows (25–50 months and over 140 months in age, respectively). When GCs were cultured under 20% O 2 for 4 days, mitochondrial DNA copy number (Mt-number), determined by real-time PCR, increased throughout the culture period, and the extent of increase was greater in the GCs of young cows than in those of old cows. In a second experiment, GCs were cultured under 20% O 2 for 24 h. Protein levels of TOMM20 and TFAM in GCs were lower in aged cows than in young cows, and the amount of reactive oxygen species and the mitochondrial membrane potential were higher, whereas ATP content and proliferation activity were lower, respectively. Glucose consumption and lactate production were higher in the GCs of aged cows than in those of young cows. When GCs were cultured under 5% or 20% O 2 for 24 h, low O 2 decreased ATP content and increased glucose consumption in GCs of both age groups compared with high O 2 ; however, low O 2 decreased the Mt-number only in the GCs of young cows. In conclusion, we show that aging affects mitochondrial quantity, function, and response to differential O 2 tensions in GCs.

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“…For this reason, future carnivore studies should examine the value of a bioreactor culture system (Heise et al. ) for in vitro follicle development. This approach, which involves a suspension system consisting of orbiting test tubes and rotating‐wall vessels, enhances nutrient and oxygen transport from the microenvironment into the ovarian tissues and has improved growth and viability of rat pre‐antral follicles (Heise et al.…”

Section: In Vitro Culture Of Primordial Follicles Within Ovarian Tissuementioning

confidence: 99%

“…This approach, which involves a suspension system consisting of orbiting test tubes and rotating‐wall vessels, enhances nutrient and oxygen transport from the microenvironment into the ovarian tissues and has improved growth and viability of rat pre‐antral follicles (Heise et al. ). Compared with a conventional static system (similar to what is now used in the dog and cat), the bioreactor approach has increased oxygen and nutrient supplies to rat follicles and allowed resident oocytes to more readily achieve meiosis (Heise et al.…”

Section: In Vitro Culture Of Primordial Follicles Within Ovarian Tissuementioning

confidence: 99%

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The Domestic Dog and Cat as Models for Understanding the Regulation of Ovarian Follicle Development In Vitro

Songsasen

Comizzoli

Nagashima

et al. 2012

Reprod Domestic Animals

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The culture of ovarian follicles is an important tool for understanding of the mechanisms controlling follicle development and differentiation of its oocyte. The benefit of recovering meiotically and developmentally competent oocytes from early stage follicles (primordial, primary, preantral and early antral) also would be significant, ranging from rescue of genomes from endangered species to preserving fertility in women facing cancer treatments. This field of research is at an early stage of scientific discovery. To-date, live offspring from cultured primordial follicles that produced fertilizable oocytes has occurred only in the mouse. Progress in other more complex species has been limited because larger animals have longer durations of natural folliculogenesis, thereby requiring more culture time to generate fully grown follicles and oocytes. We believe the dog and cat are excellent models for understanding more about folliculogenesis in vitro. This review highlights what is known about this topic for these two species as well as future priorities. In brief, it is more challenging to maintain viability of primordial follicles within ovarian tissues in vitro in the dog than the cat. Nonetheless, it is possible to grow both isolated cat and dog preantral follicles in culture. Although the follicles of both species have the capacity to increase in size and produce steroids, only cat oocytes are morphologically normal. This striking difference between the dog and cat is an area of high research priority. While much more fundamental data are required, we envision advanced technology that will allow harvesting oocytes from the vast, unused follicle stores sequestered within carnivore ovaries. These gametes have utility for reproducing genetically valuable dogs and cats that are ‘companions’ or biomedical models for investigating human disorders or for salvaging the genomes of rare canid and felid species that die before contributing to genetic management programs.

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Dynamic Oxygen Enhances Oocyte Maturation in Long-Term Follicle Culture

Cited by 28 publications

References 31 publications

Three-dimensional systems for in vitro follicular culture: overview of alginate-based matrices

Three-dimensional systems for in vitro follicular culture: overview of alginate-based matrices

Effect of aging on mitochondria and metabolism of bovine granulosa cells

The Domestic Dog and Cat as Models for Understanding the Regulation of Ovarian Follicle Development In Vitro

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